Single crystal x-ray study of leucyl-tRNA-aminoacyl-ligase using an image intensifier-aided detection system
- Princeton Univ., NJ (United States)
This thesis reports on the successful application of a high speed X-ray recording system to the study of leucyltRNA- aminoacyl-ligase crystals from baker's yeast. This protein has proved difficult to study using conventional . techniques because of its limited supply and weak diffraction pattern. The recording system used a rotation camera geometry. The X-rays were converted into light by a thin phosphor; this light was amplified by a high gain, magnetically focused image intensifier, and the diffraction pattern was recorded on photographic film. Complete 3 Å data sets were collected from one native protein crystal and from one gold derivative crystal. The recording of each complete set took approximately sixteen hours. The mean discrepancy between symmetry-related reflections was rather high, 20% to 25%. However, most'unique reflections were observed many times, and the standard deviation of their mean intensities averaged 15%. These intensities - were used to compute a difference Patterson map for the gold derivative and possible heavy-atom sites were located. The apparatus described in this thesis is therefore capable of collecting large amounts of useful X-ray data from very few protein crystals.
- Research Organization:
- Princeton Univ., NJ (United States)
- Sponsoring Organization:
- US Atomic Energy Commission (AEC)
- DOE Contract Number:
- AT(11-1)-3120
- NSA Number:
- NSA-31-013510
- OSTI ID:
- 4236509
- Report Number(s):
- COO-3120-12
- Resource Relation:
- Other Information: Thesis. Orig. Receipt Date: 30-JUN-75
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
N48110 - Life Sciences-Radiation Effects on Biochemicals-In-Vitro
60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
*PROTEINS- CRYSTAL STRUCTURE
IMAGE INTENSIFIERS
IMAGES
RECORDING SYSTEMS
X-RAY DIFFRACTION
YEASTS