Integrated solid-state rapid thermo-cycling system

Beer, N. Reginald; Johnson, Gary

Title: Integrated solid-state rapid thermo-cycling system

Patent · Tue Nov 07 00:00:00 EST 2023

OSTI ID:2293812

Beer, N. Reginald; Johnson, Gary

Techniques, systems, and devices are disclosed for implementing a portable lab system for PCR testing. An example method for operating an integrated thermal cycling system includes depositing samples into the integrated thermal cycling system that includes a thermal cycling device and an electronic interface. The thermal cycling device includes multiple wells to receive the samples to be thermally cycled, a thermoelectric cooling (TEC) element connected to the multiple wells, a substrate on which the TEC element is positioned, and a controller coupled to the TEC element. The multiple wells are positioned within the substrate that includes a thermally conductive ground positioned between adjacent wells. Supplying power to the integrated thermal cycling system, via the electronic interface, allows the multiple wells to exchange heat with the substrate and for each well to operate independently from other wells.

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Research Organization:: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC52-07NA27344

Assignee:: Lawrence Livermore National Security, LLC (Livermore, CA)

Patent Number(s):: 11,806,719

Application Number:: 17/816,886

OSTI ID:: 2293812

Resource Relation:: Patent File Date: 08/02/2022

Country of Publication:: United States

Language:: English

References (36)

Rapid Microfluidic Thermal Cycler for Nucleic Acid Amplification Beer, Neil Reginald; Vafai, Kambiz https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090226972 US Patent Application 12/270348; 20090226972	patent-application	September 2009
Method for the complete chemical synthesis and assembly of genes and genomes Evans, Glen A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20030165946 US Patent Application 10/322360; 20030165946	patent-application	September 2003
Portable Rapid Microfluidic Thermal Cycler for Extremely Fast Nucleic Acid Amplification Beer, Neil Reginald; Vafai, Kambiz; Mahjoob, Shadi https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20090226971 US Patent Application 12/270030; 20090226971	patent-application	September 2009
Thermal device, system, and method, for fluid processing device Shigeura, John; Desmond, Sean Matthew; Cox, David M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7585663 US Patent Document 7,585,663	patent	September 2009
Isothermal surface production and regulation for high heat flux applications utilizing porous inserts Khanafer, K.; Vafai, K. International Journal of Heat and Mass Transfer, Vol. 44, Issue 15, p. 2933-2947 https://doi.org/10.1016/S0017-9310(00)00336-7	journal	August 2001
A novel strategy to engineer DNA polymerases for enhanced processivity and improved performance in vitro Wang, Yan; Prosen, Dennis E.; Mei, Li Nucleic Acids Research, Vol. 32, Issue 3, p. 1197-1207 https://doi.org/10.1093/nar/gkh271	journal	February 2004
On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets Beer, N. Reginald; Wheeler, Elizabeth K.; Lee-Houghton, Lorenna Analytical Chemistry, Vol. 80, Issue 6, p. 1854-1858 https://doi.org/10.1021/ac800048k	journal	March 2008
Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems Kaltenbach, Patrick; Swedberg, Sally A.; Witt, Klaus https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5641400 US Patent Document 5,641,400	patent	June 1997
Thermal cycler for microfluidic array assays Yoder, Karl; Brenan, Colin; Linton, John https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060094108 US Patent Application 11/227425; 20060094108	patent-application	May 2006
Real-time Stadium and Sports Weather Network Copley, Rhonda L. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080270030 US Patent Application 11/740460; 20080270030	patent-application	October 2008
Sleeve reaction chamber system Northrup, M. Allen; Beeman, Barton V.; Benett, William J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7578976 US Patent Document 7,578,976	patent	August 2009
Development of a High-Speed Real-Time Polymerase Chain Reaction System Using a Circulating Water-Based Rapid Heat-Exchange Terazono, Hideyuki; Takei, Hiroyuki; Hattori, Akihiro Japanese Journal of Applied Physics, Vol. 49, Issue 6, Article No. 06GM05 https://doi.org/10.1143/JJAP.49.06GM05	journal	June 2010
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends Zhang, Chunsun; Xing, Da Nucleic Acids Research, Vol. 35, Issue 13, p. 4223-4237 https://doi.org/10.1093/nar/gkm389	journal	June 2007
Dual-sided thermal cycler Shigeura, John S.; Desmond, Sean M.; Cox, David M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100233763 US Patent Application 12/786063; 20100233763	patent-application	September 2010
Pumped fluid cooling system and method Werner, Douglas; Goodson, Kenneth; Munch, Mark https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060042785 US Patent Application 10/927800; 20060042785	patent-application	March 2006
Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes Neuzil, Pavel; Zhang, Chunyan; Pipper, Juergen Nucleic Acids Research, Vol. 34, Issue 11, p. e77-e77 https://doi.org/10.1093/nar/gkl416	journal	June 2006
Crank Arm Stop for a Cam Reel Jones, Dennis J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080022647 US Patent Application 11/772585; 20080022647	patent-application	January 2008
Performance evaluation of thermal cyclers for PCR in a rapid cycling condition Ho Kim, Young; Yang, Inchul; Bae, Young-Seuk BioTechniques, Vol. 44, Issue 4, p. 495-505 https://doi.org/10.2144/000112705	journal	April 2008
High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets Kiss, Margaret Macris; Ortoleva-Donnelly, Lori; Beer, N. Reginald Analytical Chemistry, Vol. 80, Issue 23, p. 8975-8981 https://doi.org/10.1021/ac801276c	journal	December 2008
Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture Beer, Neil; Hindson, Benjamin; Colson, Billy https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080166793 US Patent Application 11/650363; 20080166793	patent-application	July 2008
Thermal management in microfluidics using micro-Peltier junctions Maltezos, George; Johnston, Matthew; Scherer, Axel Applied Physics Letters, Vol. 87, Issue 15, Article No. 154105 https://doi.org/10.1063/1.2089174	journal	October 2005
On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets Beer, N. Reginald; Hindson, Benjamin J.; Wheeler, Elizabeth K. Analytical Chemistry, Vol. 79, Issue 22, p. 8471-8475 https://doi.org/10.1021/ac701809w	journal	November 2007
End-complementary polymerase reaction Stemmer, Willem P.; Lipshutz, Robert J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5834252 US Patent Document 5,834,252	patent	November 1998
Streaming-Based Micro/Mini Channel Electronic Cooling Techniques Zhang, Zongqin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100091459 US Patent Application 12/345699; 20100091459	patent-application	April 2010
Method of manufacturing microfluidic devices McReynolds, Richard J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5882465 US Patent Document 5,882,465	patent	March 1999
Kinetics of the DNA polymerase pyrococcus kodakaraensis Griep, M. A.; Kotera, C. A.; Nelson, R. M. Chemical Engineering Science, Vol. 61, Issue 12, p. 3885-3892 https://doi.org/10.1016/j.ces.2005.12.032	journal	June 2006
Microdroplet-based PCR enrichment for large-scale targeted sequencing Tewhey, Ryan; Warner, Jason B.; Nakano, Masakazu Nature Biotechnology, Vol. 27, Issue 11, p. 1025-1031 https://doi.org/10.1038/nbt.1583	journal	November 2009
Solid state rapid thermocycling Beer, Neil Reginald; Spadaccini, Christopher M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8720209 US Patent Document 8,720,209	patent	May 2014
Nano/Microfluidics for diagnosis of infectious diseases in developing countries Lee, Won Gu; Kim, Yun-Gon; Chung, Bong Geun Advanced Drug Delivery Reviews, Vol. 62, Issue 4-5, p. 449-457 https://doi.org/10.1016/j.addr.2009.11.016	journal	March 2010
Molecular diagnostic assay system Dority, Doug; Phan, Tien; Fromm, David https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170021356 US Patent Application 15/217920; 20170021356	patent-application	January 2017
Apparatus for heating and cooling at food serving stations Steffensen, Chris; Lyon, Tyler; Winegar, Daniel https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7665311 US Patent Document 7,665,311	patent	February 2010
Method for recombining polynucleotides Slater, Steven C.; Ng, Wing-On; Feng, Guo Hong https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20020119535 US Patent Application 09/746432; 20020119535	patent-application	August 2002
Method for carrying out a biochemical protocol in continuous flow in a microreactor Fouillet, Yves; Vauchier, Claude; Clerc, Jean-Frederic https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050282224 US Patent Application 11/197040; 20050282224	patent-application	December 2005
Novel High-Speed Real-Time PCR Method (Hyper-PCR): Results from Its Application to Adenovirus Diagnosis Fujimoto, Tsuguto; Konagaya, Masami; Enomoto, Miki Japanese Journal of Infectious Diseases, Vol. 63, Issue 1 https://doi.org/10.7883/yoken.63.31	journal	January 2010
Temperature variation apparatus Wu, Yu-Min; Tsai, Yi-Chin; Liao, Kwan-Chiao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100101237 US Patent Application 12/453306; 20100101237	patent-application	April 2010
Rapid microfluidic thermal cycler for polymerase chain reaction nucleic acid amplification Mahjoob, Shadi; Vafai, Kambiz; Beer, N. Reginald International Journal of Heat and Mass Transfer, Vol. 51, Issue 9-10, p. 2109-2122 https://doi.org/10.1016/j.ijheatmasstransfer.2007.11.014	journal	May 2008

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