Scaling up carboxylic acid production from cheese whey and brewery wastewater via methane-arrested anaerobic digestion

Wu, Haoran; Scheve, Thai; Dalke, Rachel; Holtzapple, Mark; Urgun-Demirtas, Meltem

doi:10.1016/j.cej.2022.140080

Title: Scaling up carboxylic acid production from cheese whey and brewery wastewater via methane-arrested anaerobic digestion

Journal Article · Sat Oct 29 00:00:00 EDT 2022 · Chemical Engineering Journal

DOI:https://doi.org/10.1016/j.cej.2022.140080· OSTI ID:2007753

Wu, Haoran ^[1]; Scheve, Thai ^[2]; Dalke, Rachel ^[2]; Holtzapple, Mark ^[3]; Urgun-Demirtas, Meltem ^[2]

Argonne National Laboratory (ANL), Argonne, IL (United States); Texas A & M Univ., College Station, TX (United States)
Argonne National Laboratory (ANL), Argonne, IL (United States)
Texas A & M Univ., College Station, TX (United States)

In a circular economy, organic waste streams are valuable resources for sustainably producing chemicals and fuels. This study investigates a new methane-arrested anaerobic digestion (MAAD) process that converts high-strength cheese whey and brewery wastewater into carboxylic acids. The process was developed and optimized under various bench-scale semi-continuous (fed-batch) operating conditions (e.g., retention time, organic loading rate, pH, and feed/harvest frequency). The MAAD responses to various control-failure scenarios were also systematically investigated. The highest total acid productivity was 26g/(L_liq·d) with a substrate conversion of 0.79g COD_digested/g COD_fed at a hydraulic retention time (HRT) of approximately 2 d in a 14-L digester. The most stable conditions for digester operation (HRT 3 d at pH 6.0 and 40°C) were selected for process scale-up to 100gal (~380 L). Semi-continuous, pilot-scale MAAD successfully produced a total acid concentration of 40.6+/-1.1 g/L with 8.1% acetic acid, 45.1% butyric acid, and 44.5% lactic acid. The links between wastewater characteristics, operation mode, digester scale, and microbial community structure were statistically analyzed. The results show genera Sporolactobacillus and Clostridium positively correlate with butyric acid production (Pearson correlation coefficient>0.5). Moreover, four kinetic models were developed and fit to batch MAAD experimental datasets (R²>95%) and were successfully applied to predict the total acid production in both bench-and pilot-scale semi-continuous MAADs. In conclusion, this study shows MAAD has the potential for industrial-scale applications and is a robust platform to valorize low- or negative-value waste streams into high-value bioproducts.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 2007753

Alternate ID(s):: OSTI ID: 1962167

Journal Information:: Chemical Engineering Journal, Vol. 459; ISSN 1385-8947

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
arrested methanogenesis
carboxylate platform
high-strength wastewater
kinetic modeling
microbial community
volatile fatty acids
bioprocess scale-up
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Title: Scaling up carboxylic acid production from cheese whey and brewery wastewater via methane-arrested anaerobic digestion

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