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Title: Methods of making proteins with non-standard amino acids

Patent ·
OSTI ID:1998386
;

The disclosure provides methods of making a protein having a desired non-standard amino acid incorporated at its N-terminus in a cell and methods of screening for an amino acyl tRNA synthetase variant that preferentially selects a non-standard amino acid against its standard amino acid counterpart or undesired non-standard amino acids for incorporation into a protein in a cell.

Research Organization:
Harvard Univ., Cambridge, MA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
FG02-02ER63445
Assignee:
President & Fellows of Harvard College (Cambridge, MA)
Patent Number(s):
11,649,450
Application Number:
16/627,077
OSTI ID:
1998386
Resource Relation:
Patent File Date: 06/29/2018
Country of Publication:
United States
Language:
English

References (11)

The ClpS Adaptor Mediates Staged Delivery of N-End Rule Substrates to the AAA+ ClpAP Protease journal July 2011
UBP1 Protease Mutant and its Coding Sequence, their Applications and Heterogonous Protein Expression System patent-application June 2008
Polypeptide Release Factors in Prokaryotes and Eukaryotes journal January 2002
Identification of the Protease and the Turnover Signal Responsible for Cell Cycle-Dependent Degradation of the Caulobacter FliF Motor Protein journal August 2004
Prediction of protein function from protein sequence and structure journal January 1999
Methods of Incorporating Amino Acid Analogs into Proteins patent-application December 2005
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine journal September 1999
Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo journal September 1997
Aminoacyl-transferases and the N-end rule pathway of prokaryotic/eukaryotic specificity in a human pathogen journal February 2006
Nucleotide and Amino Acid Sequences from Xenorhabdus and Uses Thereof patent-application April 2008
Engineering posttranslational proofreading to discriminate nonstandard amino acids journal January 2018

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