Methods of producing lipid-derived compounds and host cells thereof

Coradetti, Samuel; Gladden, John Michael; Liu, Di; Geiselman, Gina Marie

Title: Methods of producing lipid-derived compounds and host cells thereof

Patent · Tue Jan 24 00:00:00 EST 2023

OSTI ID:1987064

Coradetti, Samuel; Gladden, John Michael; Liu, Di; Geiselman, Gina Marie

The present disclosure relates to genetically engineered host cells and methods of producing a lipid-derived compound by employing such host cells. In particular embodiments, the host cell includes a first mutant gene encoding a cytoplasmic tRNA thiolation protein. Optionally, the host cell can include other mutant genes for decreasing fatty alcohol catabolism, decreasing re-importation of secreted fatty alcohol, or displaying other useful characteristics, as described herein.

View Patent

Cite

Export

Save

Research Organization:: Sandia National Lab. (SNL-CA), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

DOE Contract Number:: NA0003525

Assignee:: National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM)

Patent Number(s):: 11,560,576

Application Number:: 17/323,195

OSTI ID:: 1987064

Resource Relation:: Patent File Date: 05/18/2021

Country of Publication:: United States

Language:: English

References (16)

DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L ⁻¹ ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03718B	journal	January 2016
Exploiting nonionic surfactants to enhance fatty alcohol production in Rhodosporidium toruloides Liu, Di; Geiselman, Gina M.; Coradetti, Samuel Biotechnology and Bioengineering, Vol. 117, Issue 5 https://doi.org/10.1002/bit.27285	journal	February 2020
Fatty alcohols production by oleaginous yeast Fillet, Sandy; Gibert, Jordi; Suárez, Beatriz Journal of Industrial Microbiology & Biotechnology, Vol. 42, Issue 11 https://doi.org/10.1007/s10295-015-1674-x	journal	August 2015
Systems analysis of phosphate-limitation-induced lipid accumulation by the oleaginous yeast Rhodosporidium toruloides Wang, Yanan; Zhang, Sufang; Zhu, Zhiwei Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1134-8	journal	May 2018
Conversion of sugars to ionic liquids Gladden, John M.; Dutta, Tanmoy; Sun, Jian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10934568 US Patent Document 10,934,568	patent	March 2021
Ionic liquid-tolerant cellulase enzymes Gladden, John M.; Park, Joshua I.; Singer, Steven W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10378000 US Patent Document 10,378,000	patent	August 2019
Gene targets for improved enzyme production in fungi Baker, Scott E.; Magnuson, Jon K.; Reilly, Morgann C. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10934551 US Patent Document 10,934,551	patent	March 2021
Multi-Omics Driven Metabolic Network Reconstruction and Analysis of Lignocellulosic Carbon Utilization in Rhodosporidium toruloides Kim, Joonhoon; Coradetti, Samuel T.; Kim, Young-Mo Frontiers in Bioengineering and Biotechnology, Vol. 8 https://doi.org/10.3389/fbioe.2020.612832	journal	January 2021
Ionic liquid-tolerant cellulase enzymes Gladden, John M.; Park, Joshua I.; Singer, Steven W. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9803182 US Patent Document 9,803,182	patent	October 2017
Terpene synthases for biofuel production and methods thereof Wu, Weihua; Gladden, John M.; Wu, Benjamin Chiau-pin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10947563 US Patent Document 10,947,563	patent	March 2021
Transcription factors for cellulosic enzyme production Tian, Chaoguang; Shock, Teresa; Glass, N. Louise https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9441255 US Patent Document 9,441,255	patent	September 2016
A tRNA modification balances carbon and nitrogen metabolism by regulating phosphate homeostasis Gupta, Ritu; Walvekar, Adhish S.; Liang, Shun eLife, Vol. 8 https://doi.org/10.7554/eLife.44795	journal	July 2019
Microbial lipid production by Rhodosporidium toruloides under sulfate-limited conditions Wu, Siguo; Zhao, Xin; Shen, Hongwei Bioresource Technology, Vol. 102, Issue 2 https://doi.org/10.1016/j.biortech.2010.09.033	journal	January 2011
Wobble uridine modifications–a reason to live, a reason to die?! Schaffrath, Raffael; Leidel, Sebastian A. RNA Biology, Vol. 14, Issue 9 https://doi.org/10.1080/15476286.2017.1295204	journal	April 2017
Terpene synthases for biofuel production and methods thereof Wu, Weihua; Gladden, John M.; Wu, Benjamin Chiau-pin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10400254 US Patent Document 10,400,254	patent	September 2019
Cellulase complex, and glycosidase hydrolases thereof, and methods of using thereof Singer, Steven W.; Kolinko, Sebastian D.; Denzel, Evelyn https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10941388 US Patent Document 10,941,388	patent	March 2021

Similar Records

How did nature engineer the highest surface lipid accumulation among plants? Exceptional expression of acyl-lipid-associated genes for the assembly of extracellular triacylglycerol by Bayberry (Myrica pensylvanica) fruits

Journal Article · Thu Sep 01 00:00:00 EDT 2016 · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids · OSTI ID:1987064

Simpson, Jeffrey P.; Thrower, Nicholas; Ohlrogge, John B.

Mitochondrial fatty acid β‐oxidation is required for storage‐lipid catabolism in a marine diatom

Journal Article · Tue Jul 21 00:00:00 EDT 2020 · New Phytologist · OSTI ID:1987064

Jallet, Denis; Xing, Denghui; Hughes, Alexander; +4 more

Mutations That Alter the Bacterial Cell Envelope Increase Lipid Production

Journal Article · Wed Jul 05 00:00:00 EDT 2017 · mBio · OSTI ID:1987064

Lemmer, Kimberly C.; Zhang, Weiping; Langer, Samantha J.; +10 more

Title: Methods of producing lipid-derived compounds and host cells thereof

Citation Formats

References (16)

Similar Records

Related Subjects