Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

Rasala, Beth A.; Chao, Syh-Shiuan; Pier, Matthew; Barrera, Daniel J.; Mayfield, Stephen P.

doi:10.1371/journal.pone.0094028

Title: Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

Journal Article · Mon Apr 07 00:00:00 EDT 2014 · PLoS ONE

DOI:https://doi.org/10.1371/journal.pone.0094028· OSTI ID:1904797

Rasala, Beth A. ^[1]; Chao, Syh-Shiuan ^[1]; Pier, Matthew ^[1]; Barrera, Daniel J. ^[1]; Mayfield, Stephen P. ^[1]

Univ. of California, San Diego, La Jolla, CA (United States)

Transgenic microalgae have the potential to impact many diverse biotechnological industries including energy, human and animal nutrition, pharmaceuticals, health and beauty, and specialty chemicals. However, major obstacles to sophisticated genetic and metabolic engineering in algae have been the lack of well-characterized transformation vectors to direct engineered gene products to specific subcellular locations, and the inability to robustly express multiple nuclear-encoded transgenes within a single cell. Here we validate a set of genetic tools that enable protein targeting to distinct subcellular locations, and present two complementary methods for multigene engineering in the eukaryotic green microalga Chlamydomonas reinhardtii. The tools described here will enable advanced metabolic and genetic engineering to promote microalgae biotechnology and product commercialization.

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Research Organization:: Univ. of California, San Diego, CA (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE); California Energy Commission

Grant/Contract Number:: EE0003373; CILMSF #500-10-039

OSTI ID:: 1904797

Journal Information:: PLoS ONE, Vol. 9, Issue 4; ISSN 1932-6203

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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Good News for Nuclear Transgene Expression in Chlamydomonas Schroda, Michael Cells, Vol. 8, Issue 12 https://doi.org/10.3390/cells8121534	journal	November 2019
Validation of a New Multicistronic Plasmid for the Efficient and Stable Expression of Transgenes in Microalgae Molina-Márquez, Ana; Vila, Marta; Rengel, Rocío International Journal of Molecular Sciences, Vol. 21, Issue 3 https://doi.org/10.3390/ijms21030718	journal	January 2020
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Title: Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae

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