The Acyl-Proteome of Syntrophus aciditrophicus Reveals Metabolic Relationships in Benzoate Degradation

Muroski, John M.; Fu, Janine Y.; Nguyen, Hong Hanh; Wofford, Neil Q.; Mouttaki, Housna; James, Kimberly L.; McInerney, Michael J.; Gunsalus, Robert P.; Loo, Joseph A.; Ogorzalek Loo, Rachel R.

doi:10.1016/j.mcpro.2022.100215

Title: The Acyl-Proteome of Syntrophus aciditrophicus Reveals Metabolic Relationships in Benzoate Degradation

Journal Article · Fri Apr 01 00:00:00 EDT 2022 · Molecular and Cellular Proteomics

DOI:https://doi.org/10.1016/j.mcpro.2022.100215· OSTI ID:1904723

Muroski, John M. ^[1];

^[1]; Nguyen, Hong Hanh ^[2]; Wofford, Neil Q. ^[3]; Mouttaki, Housna ^[3]; James, Kimberly L. ^[3]; McInerney, Michael J. ^[3]; Gunsalus, Robert P. ^[1]; Loo, Joseph A. ^[1];

^[1]

University of California, Los Angeles, CA (United States)
TRANSMED Co Ltd, Ho Chi Minh City (Vietnam)
University of Oklahoma, Norman, OK (United States)

Syntrophus aciditrophicus is a syntrophic bacterium degrading fatty and aromatic acids into acetate, CO₂, formate, and H₂, consumed by methanogenic archaea. The syntroph’s acyllysine modifications were analyzed with a workflow avoiding antibody enrichment, enabling unbiased global acylation profiling. Seven acyl modification types were identified, six corresponding to reactive acyl-CoA species intermediates in benzoate degradation. Benzoatedegrading enzymes were also prominent among the 60 acylated proteins. The abundant acylations and active deacylases suggest that post-translational modifications directly regulate syntrophic benzoate degradation

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Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); National Science Foundation (NSF)

Grant/Contract Number:: FC02-02ER63421; 447147-EL-21341; GM007185; 1911781; DGE-1650604; GM104610; GM085402

OSTI ID:: 1904723

Journal Information:: Molecular and Cellular Proteomics, Vol. 21, Issue 4; ISSN 1535-9476

Publisher:: American Society for Biochemistry and Molecular BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
lysine acylation
syntrophs
sirtuins
syntrophus aciditrophicus

Title: The Acyl-Proteome of Syntrophus aciditrophicus Reveals Metabolic Relationships in Benzoate Degradation

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