Computational identification of HCV neutralizing antibodies with a common HCDR3 disulfide bond motif in the antibody repertoires of infected individuals

Bozhanova, Nina G.; Flyak, Andrew I.; Brown, Benjamin P.; Ruiz, Stormy E.; Salas, Jordan; Rho, Semi; Bombardi, Robin G.; Myers, Luke; Soto, Cinque; Bailey, Justin R.; Crowe Jr., James E.; Bjorkman, Pamela J.; Meiler, Jens

doi:10.1038/s41467-022-30865-9

Title: Computational identification of HCV neutralizing antibodies with a common HCDR3 disulfide bond motif in the antibody repertoires of infected individuals

Journal Article · Wed Jun 08 00:00:00 EDT 2022 · Nature Communications

DOI:https://doi.org/10.1038/s41467-022-30865-9· OSTI ID:1903965

^[1]; Flyak, Andrew I. ^[2]; Brown, Benjamin P. ^[1];

^[3]; Salas, Jordan ^[4]; Rho, Semi ^[2]; Bombardi, Robin G. ^[1];

^[1]; Soto, Cinque ^[1]; Bailey, Justin R. ^[4];

^[1];

^[2];

^[5]

Vanderbilt University, Nashville, TN (United States)
California Institute of Technology (CalTech), Pasadena, CA (United States)
California Institute of Technology (CalTech), Pasadena, CA (United States); Johns Hopkins University, Baltimore, MD (United States)
Johns Hopkins University, Baltimore, MD (United States)
Vanderbilt University, Nashville, TN (United States); Univ. of Leipzig (Germany). School of Medicine

Despite recent success in hepatitis C virus (HCV) treatment using antivirals, an HCV vaccine is still needed to prevent reinfections in treated patients, to avert the emergence of drug-resistant strains, and to provide protection for people with no access to the antiviral therapeutics. The early production of broadly neutralizing antibodies (bNAbs) associates with HCV clearance. Several potent bNAbs bind a conserved HCV glycoprotein E2 epitope using an unusual heavy chain complementarity determining region 3 (HCDR3) containing an intra-loop disulfide bond. Isolation of additional structurally-homologous bNAbs would facilitate the recognition of key determinants of such bNAbs and guide rational vaccine design. Here we report the identification of new antibodies containing an HCDR3 disulfide bond motif using computational screening with the Rosetta software. Using the newly-discovered and already-known members of this antibody family, we review the required HCDR3 amino acid composition and propose determinants for the bent versus straight HCDR3 loop conformation observed in these antibodies.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Stanford Synchrotron Radiation Lightsource (SSRL); Vanderbilt Univ., Nashville, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); Gordon and Betty Moore Foundation

Grant/Contract Number:: AC02-76SF00515; R01 AI127469; U01 AI150739; R01 AI141661; U19 AI159822; K99 AI153465; P41GM103393

OSTI ID:: 1903965

Journal Information:: Nature Communications, Vol. 13, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

References (68)

Junctional and allele-specific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody Ying, Tianlei; Prabakaran, Ponraj; Du, Lanying Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms9223	journal	September 2015
Palivizumab epitope–displaying virus-like particles protect rodents from RSV challenge Schickli, Jeanne H.; Whitacre, David C.; Tang, Roderick S. Journal of Clinical Investigation, Vol. 125, Issue 4 https://doi.org/10.1172/JCI78450	journal	March 2015
Hepatitis C Virus E2 Envelope Glycoprotein Core Structure Kong, L.; Giang, E.; Nieusma, T. Science, Vol. 342, Issue 6162 https://doi.org/10.1126/science.1243876	journal	November 2013
Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles Hsu, M.; Zhang, J.; Flint, M. Proceedings of the National Academy of Sciences, Vol. 100, Issue 12 https://doi.org/10.1073/pnas.0832180100	journal	May 2003
Structural basis for recognition of the central conserved region of RSV G by neutralizing human antibodies Jones, Harrison G.; Ritschel, Tina; Pascual, Gabriel PLOS Pathogens, Vol. 14, Issue 3 https://doi.org/10.1371/journal.ppat.1006935	journal	March 2018
Identification of Structurally Related Antibodies in Antibody Sequence Databases Using Rosetta-Derived Position-Specific Scoring Finn, Jessica A.; Dong, Jinhui; Sevy, Alexander M. Structure, Vol. 28, Issue 10 https://doi.org/10.1016/j.str.2020.07.012	journal	October 2020
Global mapping of antibody recognition of the hepatitis C virus E2 glycoprotein: Implications for vaccine design Pierce, Brian G.; Keck, Zhen-Yong; Lau, Patrick Proceedings of the National Academy of Sciences, Vol. 113, Issue 45 https://doi.org/10.1073/pnas.1614942113	journal	October 2016
Germline V-genes sculpt the binding site of a family of antibodies neutralizing human cytomegalovirus Thomson, Christy A.; Bryson, Steve; McLean, Gary R. The EMBO Journal, Vol. 27, Issue 19 https://doi.org/10.1038/emboj.2008.179	journal	September 2008
ff19SB: Amino-Acid-Specific Protein Backbone Parameters Trained against Quantum Mechanics Energy Surfaces in Solution Tian, Chuan; Kasavajhala, Koushik; Belfon, Kellon A. A. Journal of Chemical Theory and Computation, Vol. 16, Issue 1 https://doi.org/10.1021/acs.jctc.9b00591	journal	November 2019
Genetic and structural insights into broad neutralization of hepatitis C virus by human V _H 1-69 antibodies Tzarum, Netanel; Giang, Erick; Kong, Leopold Science Advances, Vol. 5, Issue 1 https://doi.org/10.1126/sciadv.aav1882	journal	January 2019
Agonistic Human Antibodies Binding to Lecithin-Cholesterol Acyltransferase Modulate High Density Lipoprotein Metabolism Gunawardane, Ruwanthi N.; Fordstrom, Preston; Piper, Derek E. Journal of Biological Chemistry, Vol. 291, Issue 6 https://doi.org/10.1074/jbc.M115.672790	journal	December 2015
Definition of a Conserved Immunodominant Domain on Hepatitis C Virus E2 Glycoprotein by Neutralizing Human Monoclonal Antibodies Keck, Zhen-Yong; Li, Ta-Kai; Xia, Jinming Journal of Virology, Vol. 82, Issue 12 https://doi.org/10.1128/JVI.02475-07	journal	April 2008
Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation Goodwin, Eileen; Gilman, Morgan S. A.; Wrapp, Daniel Immunity, Vol. 48, Issue 2 https://doi.org/10.1016/j.immuni.2018.01.005	journal	February 2018
Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes Ryckaert, Jean-Paul; Ciccotti, Giovanni; Berendsen, Herman J. C. Journal of Computational Physics, Vol. 23, Issue 3 https://doi.org/10.1016/0021-9991(77)90098-5	journal	March 1977
Functional convergence of a germline-encoded neutralizing antibody response in rhesus macaques immunized with HCV envelope glycoproteins Chen, Fang; Tzarum, Netanel; Lin, Xiaohe Immunity, Vol. 54, Issue 4 https://doi.org/10.1016/j.immuni.2021.02.013	journal	April 2021
High frequency of shared clonotypes in human B cell receptor repertoires Soto, Cinque; Bombardi, Robin G.; Branchizio, Andre Nature https://doi.org/10.1038/s41586-019-0934-8	journal	February 2019
Accurate and predictive antibody repertoire profiling by molecular amplification fingerprinting Khan, Tarik A.; Friedensohn, Simon; Gorter de Vries, Arthur R. Science Advances, Vol. 2, Issue 3 https://doi.org/10.1126/sciadv.1501371	journal	March 2016
Flexbar 3.0 – SIMD and multicore parallelization Roehr, Johannes T.; Dieterich, Christoph; Reinert, Knut Bioinformatics, Vol. 33, Issue 18 https://doi.org/10.1093/bioinformatics/btx330	journal	May 2017
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Plasma deconvolution identifies broadly neutralizing antibodies associated with hepatitis C virus clearance Kinchen, Valerie J.; Massaccesi, Guido; Flyak, Andrew I. Journal of Clinical Investigation, Vol. 129, Issue 11 https://doi.org/10.1172/JCI130720	journal	September 2019
Proof of principle for epitope-focused vaccine design Correia, Bruno E.; Bates, John T.; Loomis, Rebecca J. Nature, Vol. 507, Issue 7491 https://doi.org/10.1038/nature12966	journal	February 2014
An alternate conformation of HCV E2 neutralizing face as an additional vaccine target Tzarum, Netanel; Giang, Erick; Kadam, Rameshwar U. Science Advances, Vol. 6, Issue 30 https://doi.org/10.1126/sciadv.abb5642	journal	July 2020
Synergistic anti-HCV broadly neutralizing human monoclonal antibodies with independent mechanisms Mankowski, Madeleine C.; Kinchen, Valerie J.; Wasilewski, Lisa N. Proceedings of the National Academy of Sciences, Vol. 115, Issue 1 https://doi.org/10.1073/pnas.1718441115	journal	December 2017
An ultralong CDRH2 in HCV neutralizing antibody demonstrates structural plasticity of antibodies against E2 glycoprotein Flyak, Andrew I.; Ruiz, Stormy E.; Salas, Jordan eLife, Vol. 9 https://doi.org/10.7554/eLife.53169	journal	March 2020
PyEMMA 2: A Software Package for Estimation, Validation, and Analysis of Markov Models Scherer, Martin K.; Trendelkamp-Schroer, Benjamin; Paul, Fabian Journal of Chemical Theory and Computation, Vol. 11, Issue 11 https://doi.org/10.1021/acs.jctc.5b00743	journal	October 2015
Immunological memory to hyperphosphorylated tau in asymptomatic individuals Pascual, Gabriel; Wadia, Jehangir S.; Zhu, Xueyong Acta Neuropathologica, Vol. 133, Issue 5 https://doi.org/10.1007/s00401-017-1705-y	journal	March 2017
Die Primärstruktur des kristallisierbaren monoklonalen Immunglobulins IgG₁Kol. II. Aminosäuresequenz der L-Kette, λ-Typ, Subgruppe I Kratzin, Hartmut D.; Palm, Walter; Stangel, Martin Biological Chemistry Hoppe-Seyler, Vol. 370, Issue 1 https://doi.org/10.1515/bchm3.1989.370.1.263	journal	January 1989
Can Broadly Neutralizing Monoclonal Antibodies Lead to a Hepatitis C Virus Vaccine? Kinchen, Valerie J.; Cox, Andrea L.; Bailey, Justin R. Trends in Microbiology, Vol. 26, Issue 10 https://doi.org/10.1016/j.tim.2018.04.002	journal	October 2018
Structures of Preferred Human IgV Genes–Based Protective Antibodies Identify How Conserved Residues Contact Diverse Antigens and Assign Source of Specificity to CDR3 Loop Variation Bryson, Steve; Thomson, Christy A.; Risnes, Louise F. The Journal of Immunology, Vol. 196, Issue 11 https://doi.org/10.4049/jimmunol.1402890	journal	April 2016
Broadly neutralizing antibodies protect against hepatitis C virus quasispecies challenge Law, Mansun; Maruyama, Toshiaki; Lewis, Jamie Nature Medicine, Vol. 14, Issue 1 https://doi.org/10.1038/nm1698	journal	December 2007
Length-independent structural similarities enrich the antibody CDR canonical class model Nowak, Jaroslaw; Baker, Terry; Georges, Guy mAbs, Vol. 8, Issue 4 https://doi.org/10.1080/19420862.2016.1158370	journal	March 2016
Long-Time-Step Molecular Dynamics through Hydrogen Mass Repartitioning Hopkins, Chad W.; Le Grand, Scott; Walker, Ross C. Journal of Chemical Theory and Computation, Vol. 11, Issue 4 https://doi.org/10.1021/ct5010406	journal	March 2015
Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: Report of the BIOMED-2 Concerted Action BMH4-CT98-3936 van Dongen, J. J. M.; Langerak, A. W.; Brüggemann, M. Leukemia, Vol. 17, Issue 12 https://doi.org/10.1038/sj.leu.2403202	journal	December 2003
A Pareto-Optimal Refinement Method for Protein Design Scaffolds Nivón, Lucas Gregorio; Moretti, Rocco; Baker, David PLoS ONE, Vol. 8, Issue 4 https://doi.org/10.1371/journal.pone.0059004	journal	April 2013
iMOSFLM : a new graphical interface for diffraction-image processing with MOSFLM Battye, T. Geoff G.; Kontogiannis, Luke; Johnson, Owen Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910048675	journal	March 2011
Error filtering, pair assembly and error correction for next-generation sequencing reads Edgar, Robert C.; Flyvbjerg, Henrik Bioinformatics, Vol. 31, Issue 21 https://doi.org/10.1093/bioinformatics/btv401	journal	July 2015
Neutralizing antibody response during acute and chronic hepatitis C virus infection Logvinoff, C.; Major, M. E.; Oldach, D. Proceedings of the National Academy of Sciences, Vol. 101, Issue 27 https://doi.org/10.1073/pnas.0403519101	journal	June 2004
Novel therapies for hepatitis C — one pill fits all? Manns, Michael P.; von Hahn, Thomas Nature Reviews Drug Discovery, Vol. 12, Issue 8 https://doi.org/10.1038/nrd4050	journal	June 2013
Broadly neutralizing antibodies with few somatic mutations and hepatitis C virus clearance Bailey, Justin R.; Flyak, Andrew I.; Cohen, Valerie J. JCI Insight, Vol. 2, Issue 9 https://doi.org/10.1172/jci.insight.92872	journal	May 2017
Broadly neutralizing antibodies from an individual that naturally cleared multiple hepatitis C virus infections uncover molecular determinants for E2 targeting and vaccine design Keck, Zhen-Yong; Pierce, Brian G.; Lau, Patrick PLOS Pathogens, Vol. 15, Issue 5 https://doi.org/10.1371/journal.ppat.1007772	journal	May 2019
Glycan Masking of Hemagglutinin for Adenovirus Vector and Recombinant Protein Immunizations Elicits Broadly Neutralizing Antibodies against H5N1 Avian Influenza Viruses Lin, Shih-Chang; Liu, Wen-Chun; Jan, Jia-Tsrong PLoS ONE, Vol. 9, Issue 3 https://doi.org/10.1371/journal.pone.0092822	journal	March 2014
Stereotyped antibody responses target posttranslationally modified gluten in celiac disease Snir, Omri; Chen, Xi; Gidoni, Moriah JCI Insight, Vol. 2, Issue 17 https://doi.org/10.1172/jci.insight.93961	journal	September 2017
MolProbity : all-atom structure validation for macromolecular crystallography Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1 https://doi.org/10.1107/S0907444909042073	journal	December 2009
Coot model-building tools for molecular graphics Emsley, Paul; Cowtan, Kevin Acta Crystallographica Section D Biological Crystallography, Vol. 60, Issue 12, p. 2126-2132 https://doi.org/10.1107/S0907444904019158	journal	November 2004
HIV vaccine delayed boosting increases Env variable region 2–specific antibody effector functions Easterhoff, David; Pollara, Justin; Luo, Kan JCI Insight, Vol. 5, Issue 2 https://doi.org/10.1172/jci.insight.131437	journal	January 2020
Recognition of a highly conserved glycoprotein B epitope by a bivalent antibody neutralizing HCMV at a post-attachment step Ye, Xiaohua; Su, Hang; Wrapp, Daniel PLOS Pathogens, Vol. 16, Issue 8 https://doi.org/10.1371/journal.ppat.1008736	journal	August 2020
A Diverse Panel of Hepatitis C Virus Glycoproteins for Use in Vaccine Research Reveals Extremes of Monoclonal Antibody Neutralization Resistance Urbanowicz, Richard A.; McClure, C. Patrick; Brown, Richard J. P. Journal of Virology, Vol. 90, Issue 7 https://doi.org/10.1128/JVI.02700-15	journal	April 2016
Neutralization mechanism of human monoclonal antibodies against Rift Valley fever virus Wang, Qihui; Ma, Tong; Wu, Yan Nature Microbiology, Vol. 4, Issue 7 https://doi.org/10.1038/s41564-019-0411-z	journal	April 2019
A Structurally Distinct Human Mycoplasma Protein that Generically Blocks Antigen-Antibody Union Grover, R. K.; Zhu, X.; Nieusma, T. Science, Vol. 343, Issue 6171 https://doi.org/10.1126/science.1246135	journal	February 2014
PHENIX: a comprehensive Python-based system for macromolecular structure solution Adams, Paul D.; Afonine, Pavel V.; Bunkóczi, Gábor Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2, p. 213-221 https://doi.org/10.1107/S0907444909052925	journal	January 2010
Importance of Neutralizing Monoclonal Antibodies Targeting Multiple Antigenic Sites on the Middle East Respiratory Syndrome Coronavirus Spike Glycoprotein To Avoid Neutralization Escape Wang, Lingshu; Shi, Wei; Chappell, James D. Journal of Virology, Vol. 92, Issue 10 https://doi.org/10.1128/JVI.02002-17	journal	March 2018
Introduction: A smouldering public-health crisis Gravitz, Lauren Nature, Vol. 474, Issue 7350 https://doi.org/10.1038/474S2a	journal	June 2011
Selection Pressure From Neutralizing Antibodies Drives Sequence Evolution During Acute Infection With Hepatitis C Virus Dowd, Kimberly A.; Netski, Dale M.; Wang, Xiao–Hong Gastroenterology, Vol. 136, Issue 7 https://doi.org/10.1053/j.gastro.2009.02.080	journal	June 2009
Affinity maturation in a human humoral response to influenza hemagglutinin McCarthy, Kevin R.; Raymond, Donald D.; Do, Khoi T. Proceedings of the National Academy of Sciences, Vol. 116, Issue 52 https://doi.org/10.1073/pnas.1915620116	journal	December 2019
Structure and mechanism of monoclonal antibody binding to the junctional epitope of Plasmodium falciparum circumsporozoite protein Oyen, David; Torres, Jonathan L.; Aoto, Phillip C. PLOS Pathogens, Vol. 16, Issue 3 https://doi.org/10.1371/journal.ppat.1008373	journal	March 2020
Hepatitis C virus Broadly Neutralizing Monoclonal Antibodies Isolated 25 Years after Spontaneous Clearance Merat, Sabrina J.; Molenkamp, Richard; Wagner, Koen PLOS ONE, Vol. 11, Issue 10 https://doi.org/10.1371/journal.pone.0165047	journal	October 2016
Building Water Models: A Different Approach Izadi, Saeed; Anandakrishnan, Ramu; Onufriev, Alexey V. The Journal of Physical Chemistry Letters, Vol. 5, Issue 21 https://doi.org/10.1021/jz501780a	journal	October 2014
Rosetta3: An Object-Oriented Software Suite for the Simulation and Design of Macromolecules Leaver-Fay, Andrew; Tyka, Michael; Lewis, Steven M. Computer Methods, Part C https://doi.org/10.1016/B978-0-12-381270-4.00019-6	book	January 2011
Rapid induction of virus-neutralizing antibodies and viral clearance in a single-source outbreak of hepatitis C Pestka, J. M.; Zeisel, M. B.; Blaser, E. Proceedings of the National Academy of Sciences, Vol. 104, Issue 14 https://doi.org/10.1073/pnas.0607026104	journal	March 2007
Determination of Alkali and Halide Monovalent Ion Parameters for Use in Explicitly Solvated Biomolecular Simulations Joung, In Suk; Cheatham, Thomas E. The Journal of Physical Chemistry B, Vol. 112, Issue 30 https://doi.org/10.1021/jp8001614	journal	July 2008
HCV Broadly Neutralizing Antibodies Use a CDRH3 Disulfide Motif to Recognize an E2 Glycoprotein Site that Can Be Targeted for Vaccine Design Flyak, Andrew I.; Ruiz, Stormy; Colbert, Michelle D. Cell Host & Microbe, Vol. 24, Issue 5 https://doi.org/10.1016/j.chom.2018.10.009	journal	November 2018
Awareness of infection, knowledge of hepatitis C, and medical follow-up among individuals testing positive for hepatitis C: National Health and Nutrition Examination Survey 2001-2008 Denniston, Maxine M.; Klevens, R. Monina; McQuillan, Geraldine M. Hepatology, Vol. 55, Issue 6 https://doi.org/10.1002/hep.25556	journal	April 2012
PyIR: a scalable wrapper for processing billions of immunoglobulin and T cell receptor sequences using IgBLAST Soto, Cinque; Finn, Jessica A.; Willis, Jordan R. BMC Bioinformatics, Vol. 21, Issue 1 https://doi.org/10.1186/s12859-020-03649-5	journal	July 2020
Broadly Neutralizing Antibodies Targeting New Sites of Vulnerability in Hepatitis C Virus E1E2 Colbert, Michelle D.; Flyak, Andrew I.; Ogega, Clinton O. Journal of Virology, Vol. 93, Issue 14 https://doi.org/10.1128/JVI.02070-18	journal	May 2019
IgBLAST: an immunoglobulin variable domain sequence analysis tool Ye, Jian; Ma, Ning; Madden, Thomas L. Nucleic Acids Research, Vol. 41, Issue W1 https://doi.org/10.1093/nar/gkt382	journal	May 2013
Long antibody HCDR3s from HIV-naïve donors presented on a PG9 neutralizing antibody background mediate HIV neutralization Willis, Jordan R.; Finn, Jessica A.; Briney, Bryan Proceedings of the National Academy of Sciences, Vol. 113, Issue 16 https://doi.org/10.1073/pnas.1518405113	journal	April 2016
Discovery of Marburg virus neutralizing antibodies from virus-naïve human antibody repertoires using large-scale structural predictions Bozhanova, Nina G.; Sangha, Amandeep K.; Sevy, Alexander M. Proceedings of the National Academy of Sciences, Vol. 117, Issue 49 https://doi.org/10.1073/pnas.1922654117	journal	November 2020
Clearance of hepatitis C infection is associated with the early appearance of broad neutralizing antibody responses: Hepatology Osburn, William O.; Snider, Anna E.; Wells, Brittany L. Hepatology, Vol. 59, Issue 6 https://doi.org/10.1002/hep.27013	journal	April 2014

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Title: Computational identification of HCV neutralizing antibodies with a common HCDR3 disulfide bond motif in the antibody repertoires of infected individuals

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