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Title: Microbial conversion of methane

Patent ·
OSTI ID:1892524

This invention relates to a process for producing lipids and amino acids from a gaseous substrate comprising methane and oxygen. The process uses a culture of a methanotrophic microorganism in a liquid nutrient medium. The methanotrophic microorganism can be a Methylomicrobium bacterium and more specifically Methylomicrobium buryatense 5GB1. The lipid products can be in the cellular membrane of the methanotroph and can be extracted in a separate extraction zone.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States); LanzaTech New Zealand Ltd., Skokie, IL (United States); Univ. of Washington, Seattle, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC36-08GO28308; AR0000350
Assignee:
LanzaTech New Zealand Limited (Skokie, IL); University of Washington through its Center for Commercialization (Seattle, WA); Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Number(s):
11,220,700
Application Number:
14/519,075
OSTI ID:
1892524
Resource Relation:
Patent File Date: 10/20/2014
Country of Publication:
United States
Language:
English

References (23)

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Genetic Systems for Moderately Halo(alkali)philic Bacteria of the Genus Methylomicrobium book January 2011
Hydrogen isotope fractionation in lipids of the methane-oxidizing bacterium Methylococcus capsulatus journal November 2002
Taxonomic Characterization of New Alkaliphilic and Alkalitolerant Methanotrophs from Soda Lakes of the Southeastern Transbaikal Region and description of Methylomicrobium buryatense sp.nov. journal January 2001
Biological production of acetic acid from waste gases with Clostridium ljungdahlii patent September 1998
The Effect of Copper Ions on Membrane Content and Methane Monooxygenase Activity in Methanol-grown Cells of Methylococcus capsulatus (Bath) journal January 1985
Biological treatment method for water patent March 1995
Method patent-application July 2003
Microorganism growth substrate comprising a biomass derived from methanotrophic bacteria patent September 2010
Method for the Positive Selection of Chromosomal Mutations in C1 Metabolizing Bacteria Via Homologous Recombination patent-application March 2006
Simulation of growth of methane-utilising bacteria in batch culture journal April 2007
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Biorefinery system, methods and compositions thereof patent-application January 2014
Biological production of products from waste gases patent January 2002
Product patent-application October 2005
Microbial process for the preparation of acetic acid as well as solvent for its extraction from the fermentation broth patent April 2002
Oleaginous yeast Cryptococcus curvatus culture with dark fermentation hydrogen production effluent as feedstock for microbial lipid production journal August 2011
Methane-oxidizing Activity and Membrane Morphology in a Methanolgrown Obligate Methanotroph, Methylosinus trichosporium OB3b journal July 1981

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