Geobacter sp. Strain IAE Dihaloeliminates 1,1,2-Trichloroethane and 1,2-Dichloroethane

Jiang, Lisi; Yang, Yi; Jin, Huijuan; Wang, Hongyan; Swift, Cynthia M.; Xie, Yongchao; Schubert, Torsten; Löffler, Frank E.; Yan, Jun

doi:10.1021/acs.est.1c05952

Title: Geobacter sp. Strain IAE Dihaloeliminates 1,1,2-Trichloroethane and 1,2-Dichloroethane

Journal Article · Thu Mar 03 00:00:00 EST 2022 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.1c05952· OSTI ID:1860551

Jiang, Lisi ^[1];

^[2]; Jin, Huijuan ^[3]; Wang, Hongyan ^[3]; Swift, Cynthia M. ^[4]; Xie, Yongchao ^[4]; Schubert, Torsten ^[5];

^[6];

^[7]

Chinese Academy of Sciences (CAS), Beijing (China); Univ. of Chinese Academy of Sciences, Shenyang (China)
Chinese Academy of Sciences (CAS), Beijing (China)
Univ. of Chinese Academy of Sciences, Shenyang (China); Univ. of Chinese Academy of Sciences, Beijing (China)
Univ. of Tennessee, Knoxville, TN (United States)
Friedrich Schiller Univ., Jena (Germany)
Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of Chinese Academy of Sciences, Shenyang (China)

We report that chlorinated ethanes, including 1,2-dichloroethane (1,2-DCA) and 1,1,2-trichloroethane (1,1,2-TCA), are widespread groundwater contaminants. Enrichment cultures XR_DCA and XR_TCA derived from river sediment dihaloeliminated 1,2-DCA to ethene and 1,1,2-TCA to vinyl chloride (VC), respectively. The XR_TCA culture subsequently converted VC to ethene via hydrogenolysis. Microbial community profiling demonstrated the enrichment of Geobacter 16S rRNA gene sequences in both the XR_DCAand XR_TCA cultures, and Dehalococcoides mccartyi (Dhc) sequences were only detected in the ethene-producing XR_TCA culture. The presence of a novel Geobacter population, designated as Geobacter sp. strain IAE, was identified by the 16S rRNA gene-targeted polymerase chain reaction and Sanger sequencing. Time-resolved population dynamics attributed the dihaloelimination activity to strain IAE, which attained the growth yields of 0.93 ± 0.06 x 10⁷ and 1.18 ± 0.14 x 10⁷ cells per μmol Cl^-1 released with 1,2-DCA and 1,1,2-TCA as electron acceptors, respectively. In contrast, Dhc growth only occurred during VC-to-ethene hydrogenolysis. Our findings discover a Geobacter sp. strain capable of respiring multiple chlorinated ethanes and demonstrate the involvement of a broader diversity of organohalide-respiring bacteria in the detoxification of 1,2-DCA and 1,1,2-TCA.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States); Chinese Academy of Sciences (CAS), Beijing (China); Univ. of Chinese Academy of Sciences, Shenyang (China)

Sponsoring Organization:: USDOE; National Key Research and Development Program of China; Natural Science Foundation of China; Liaoning Revitalization Talents Program; Chinese Academy of Sciences; University Consortium for Field-Focused Groundwater Research

Grant/Contract Number:: AC05-00OR22725; 2018YFC1800704; 2019YFC1804400; 41977295; 41907287; XLYC1807139; ZDBS-LY-DQC038

OSTI ID:: 1860551

Journal Information:: Environmental Science and Technology, Vol. 56, Issue 6; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
geobacter
chlorinated ethanes
dihaloelimination
1
1
2-trichloroethane
1
2-dichloroethane
organohalide respiration
bioremediation

Title: Geobacter sp. Strain IAE Dihaloeliminates 1,1,2-Trichloroethane and 1,2-Dichloroethane

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