Deconstruction of Woody Biomass via Protic and Aprotic Ionic Liquid Pretreatment for Ethanol Production

Das, Lalitendu; Achinivu, Ezinne C.; Barcelos, Carolina Araujo; Sundstrom, Eric; Amer, Bashar; Baidoo, Edward K.; Simmons, Blake A.; Sun, Ning; Gladden, John M.

doi:10.1021/acssuschemeng.0c07925

Title: Deconstruction of Woody Biomass via Protic and Aprotic Ionic Liquid Pretreatment for Ethanol Production

Journal Article · Sun Mar 14 00:00:00 EST 2021 · ACS Sustainable Chemistry & Engineering

DOI:https://doi.org/10.1021/acssuschemeng.0c07925· OSTI ID:1845822

Das, Lalitendu ^[1]; Achinivu, Ezinne C. ^[1]; Barcelos, Carolina Araujo ^[2];

^[2]; Amer, Bashar ^[3]; Baidoo, Edward K. ^[3];

^[3];

^[2];

^[1]

Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Ionic liquids (ILs) have emerged as important solvents for conversion of lignocellulosic feedstocks to fuels and chemicals due to their ability to enable efficient biomass deconstruction and fractionation. Woody biomass derived from forest and agricultural residues has the potential to be used for production of biofuels and its removal from forests can help mitigate disastrous wildfires in fire-prone states like California. This study evaluated woody biomass types (pine, almond, walnut, and fir) from California as potential biofuel feedstocks. The feedstocks were pretreated with the ILs cholinium lysinate ([Ch][Lys]) and ethanolamine acetate ([EOA][OAc]), followed by enzymatic hydrolysis and fermentation of lignocellulosic sugars to produce ethanol. Under optimal conditions, [EOA][OAc] pretreatment and enzymatic hydrolysis generated glucose and xylose yields in the range of 24-82 and 14-80%, respectively, while glucose and xylose yields for the [Ch][Lys] ranged between 28-83 and 23-80%, respectively. Maximum fermentable sugar was released from almond wood, and the lowest amount was from pine and fir. Further, blends of feedstocks were also explored, and a blend with a mass ratio of 2/2/1 (almond/walnut/pine) resulted in maximum glucose and xylose (>90%) yields using [Ch][Lys]. Fermentation of this hydrolysate using a C5-utilizing strain of Saccharomyces cerevisiae resulted in a maximum ethanol concentration of 17.9 g/L for mixture biomass hydrolysate, corresponding to 60.8% fermentation efficiency. This study represents the first demonstration of the use of these ILs for pretreatment of woody biomass blends that resulted in a high overall conversion efficiency for ethanol production.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)

Sponsoring Organization:: California Energy Commission; USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC02-05CH11231; GFO-17-902

OSTI ID:: 1845822

Journal Information:: ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 12; ISSN 2168-0485

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

References (44)

Structural modification of pine and poplar wood by alkali pretreatment to improve ethanol production Bay, Mohammad Saber; Karimi, Keikhosro; Nasr Esfahany, Mohsen Industrial Crops and Products, Vol. 152 https://doi.org/10.1016/j.indcrop.2020.112506	journal	September 2020
Biomass deconstruction to sugars Blanch, Harvey W.; Simmons, Blake A.; Klein-Marcuschamer, Daniel Biotechnology Journal, Vol. 6, Issue 9 https://doi.org/10.1002/biot.201000180	journal	August 2011
Aprotic vs Protic Ionic Liquids for Lignocellulosic Biomass Pretreatment: Anion Effects, Enzymatic Hydrolysis, Solid-State NMR, Distillation, and Recycle Hossain, Md. Mokarrom; Rawal, Aditya; Aldous, Leigh ACS Sustainable Chemistry & Engineering https://doi.org/10.1021/acssuschemeng.8b05987	journal	June 2019
Lignin as a base material for materials applications: Chemistry, application and economics Stewart, Derek Industrial Crops and Products, Vol. 27, Issue 2 https://doi.org/10.1016/j.indcrop.2007.07.008	journal	March 2008
Dissolution of Cellose with Ionic Liquids Swatloski, Richard P.; Spear, Scott K.; Holbrey, John D. Journal of the American Chemical Society, Vol. 124, Issue 18, p. 4974-4975 https://doi.org/10.1021/ja025790m	journal	May 2002
Characterization of bioactive compounds in the biomass of black locust, poplar and willow Tyśkiewicz, Katarzyna; Konkol, Marcin; Kowalski, Rafał Trees, Vol. 33, Issue 5 https://doi.org/10.1007/s00468-019-01837-2	journal	April 2019
Transforming biomass conversion with ionic liquids: process intensification and the development of a high-gravity, one-pot process for the production of cellulosic ethanol Xu, Feng; Sun, Jian; Konda, N. V. S. N. Murthy Energy & Environmental Science, Vol. 9, Issue 3 https://doi.org/10.1039/C5EE02940F	journal	January 2016
Effects of biomass particle size on steam explosion pretreatment performance for improving the enzyme digestibility of corn stover Liu, Zhi-Hua; Qin, Lei; Pang, Feng Industrial Crops and Products, Vol. 44, p. 176-184 https://doi.org/10.1016/j.indcrop.2012.11.009	journal	January 2013
Effects of Particle Size on Biomass Pretreatment for Biofuel Production Yang, Yang; Sun, Mingman; Deines, Timothy ASME 2019 14th International Manufacturing Science and Engineering Conference, Volume 2: Processes; Materials https://doi.org/10.1115/MSEC2019-2916	conference	November 2019
About Making Lignin Great Again—Some Lessons From the Past Glasser, Wolfgang G. Frontiers in Chemistry, Vol. 7 https://doi.org/10.3389/fchem.2019.00565	journal	August 2019
Recent advances in the pretreatment of lignocellulosic biomass Tu, Wei-Chien; Hallett, Jason P. Current Opinion in Green and Sustainable Chemistry, Vol. 20 https://doi.org/10.1016/j.cogsc.2019.07.004	journal	December 2019
Understanding the role of water during ionic liquid pretreatment of lignocellulose: co-solvent or anti-solvent? Shi, Jian; Balamurugan, Kanagasabai; Parthasarathi, Ramakrishnan Green Chem., Vol. 16, Issue 8 https://doi.org/10.1039/C4GC00373J	journal	January 2014
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose Socha, A. M.; Parthasarathi, R.; Shi, J. Proceedings of the National Academy of Sciences, Vol. 111, Issue 35 https://doi.org/10.1073/pnas.1405685111	journal	August 2014
Approaches for More Efficient Biological Conversion of Lignocellulosic Feedstocks to Biofuels and Bioproducts Baral, Nawa Raj; Sundstrom, Eric R.; Das, Lalitendu ACS Sustainable Chemistry & Engineering, Vol. 7, Issue 10 https://doi.org/10.1021/acssuschemeng.9b01229	journal	April 2019
Progress on the pre-treatment of lignocellulosic biomass employing ionic liquids Halder, Pobitra; Kundu, Sazal; Patel, Savankumar Renewable and Sustainable Energy Reviews, Vol. 105 https://doi.org/10.1016/j.rser.2019.01.052	journal	May 2019
Ionic liquids: Promising green solvents for lignocellulosic biomass utilization Yoo, Chang Geun; Pu, Yunqiao; Ragauskas, Arthur J. Current Opinion in Green and Sustainable Chemistry, Vol. 5 https://doi.org/10.1016/j.cogsc.2017.03.003	journal	June 2017
Understanding the synergistic effect and the main factors influencing the enzymatic hydrolyzability of corn stover at low enzyme loading by hydrothermal and/or ultrafine grinding pretreatment Zhang, Haiyan; Li, Junbao; Huang, Guangqun Bioresource Technology, Vol. 264 https://doi.org/10.1016/j.biortech.2018.05.090	journal	September 2018
Woody biomass energy potential in 2050 Lauri, Pekka; Havlík, Petr; Kindermann, Georg Energy Policy, Vol. 66 https://doi.org/10.1016/j.enpol.2013.11.033	journal	March 2014
Understanding Lignin Fractionation and Characterization from Engineered Switchgrass Treated by an Aqueous Ionic Liquid Liu, Enshi; Li, Mi; Das, Lalitendu ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 5 https://doi.org/10.1021/acssuschemeng.8b00384	journal	March 2018
Scale-up and evaluation of high solid ionic liquid pretreatment and enzymatic hydrolysis of switchgrass Li, Chenlin; Tanjore, Deepti; He, Wei Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-154	journal	January 2013
Deconstruction of Lignocellulosic Biomass to Fuels and Chemicals Chundawat, Shishir P. S.; Beckham, Gregg T.; Himmel, Michael E. Annual Review of Chemical and Biomolecular Engineering, Vol. 2, Issue 1, p. 121-145 https://doi.org/10.1146/annurev-chembioeng-061010-114205	journal	July 2011
The critical role of lignin in lignocellulosic biomass conversion and recent pretreatment strategies: A comprehensive review Yoo, Chang Geun; Meng, Xianzhi; Pu, Yunqiao Bioresource Technology, Vol. 301 https://doi.org/10.1016/j.biortech.2020.122784	journal	April 2020
Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies Pallapolu, Venkata Ramesh; Lee, Y. Y.; Garlock, Rebecca J. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.03.085	journal	December 2011
Impact of mixed feedstocks and feedstock densification on ionic liquid pretreatment efficiency Shi, Jian; Thompson, Vicki S.; Yancey, Neal A. Biofuels, Vol. 4, Issue 1 https://doi.org/10.4155/bfs.12.82	journal	January 2013
Comparison of sugar content for ionic liquid pretreated Douglas-fir woodchips and forestry residues Socha, Aaron M.; Plummer, Samuel P.; Stavila, Vitalie Biotechnology for Biofuels, Vol. 6, Issue 1 https://doi.org/10.1186/1754-6834-6-61	journal	January 2013
Protic, Aprotic, and Choline-Derived Ionic Liquids: Toward Enhancing the Accessibility of Hardwood and Softwood Rigual, Victoria; Ovejero-Pérez, Antonio; Rivas, Sandra ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 3 https://doi.org/10.1021/acssuschemeng.9b04443	journal	August 2019
Pretreatment of biomass using ionic liquids: Research updates Zhang, Quanguo; Hu, Jianjun; Lee, Duu-Jong Renewable Energy, Vol. 111 https://doi.org/10.1016/j.renene.2017.03.093	journal	October 2017
Comparative Evaluation of Industrial Hemp Cultivars: Agronomical Practices, Feedstock Characterization, and Potential for Biofuels and Bioproducts Das, Lalitendu; Li, Wenqi; Dodge, Luke A. ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 16 https://doi.org/10.1021/acssuschemeng.9b06145	journal	March 2020
Woody biomass pretreatment for cellulosic ethanol production: Technology and energy consumption evaluation☆ Zhu, J. Y.; Pan, X. J. Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2009.11.007	journal	July 2010
Correction: Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli Eudes, Aymerick; Juminaga, Darmawi; Baidoo, Edward E. K. Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/1475-2859-13-8	journal	January 2014
Whole plant cell wall characterization using solution-state 2D NMR Mansfield, Shawn D.; Kim, Hoon; Lu, Fachuang Nature Protocols, Vol. 7, Issue 9 https://doi.org/10.1038/nprot.2012.064	journal	August 2012
Effect of Lignin Chemistry on the Enzymatic Hydrolysis of Woody Biomass Yu, Zhiying; Gwak, Ki-Seob; Treasure, Trevor ChemSusChem, Vol. 7, Issue 7 https://doi.org/10.1002/cssc.201400042	journal	June 2014
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli Eudes, Aymerick; Juminaga, Darmawi; Baidoo, Edward E. K. Microbial Cell Factories, Vol. 12, Issue 1 https://doi.org/10.1186/1475-2859-12-62	journal	January 2013
Performance of three delignifying pretreatments on hardwoods: hydrolysis yields, comprehensive mass balances, and lignin properties Bhalla, Aditya; Cai, Charles M.; Xu, Feng Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1546-0	journal	September 2019
Volatile terpene extraction of spruce, fir and maritime pine wood: supercritical CO₂ extraction compared to classical solvent extractions and steam distillation Bertaud, Frédérique; Crampon, Christelle; Badens, Elisabeth Holzforschung, Vol. 71, Issue 7-8 https://doi.org/10.1515/hf-2016-0197	journal	May 2017
Valorization of residual walnut biomass from forest management and wood processing for the production of bioactive compounds Fernández-Agulló, Adela; Freire, M. Sonia; Ramírez-López, Cristina Biomass Conversion and Biorefinery, Vol. 11, Issue 2 https://doi.org/10.1007/s13399-019-00598-9	journal	January 2020
Characterization of Lignin Streams during Bionic Liquid-Based Pretreatment from Grass, Hardwood, and Softwood Dutta, Tanmoy; Papa, Gabriella; Wang, Eileen ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 3 https://doi.org/10.1021/acssuschemeng.7b02991	journal	January 2018
One-pot integrated biofuel production using low-cost biocompatible protic ionic liquids Sun, Jian; Konda, N. V. S. N. Murthy; Parthasarathi, Ramakrishnan Green Chemistry, Vol. 19, Issue 13 https://doi.org/10.1039/C7GC01179B	journal	January 2017
Can We Reduce the Cellulase Enzyme Loading Required To Achieve Efficient Lignocellulose Deconstruction by Only Using the Initially Absorbed Enzymes? Hu, Jinguang; Mok, Yiu Ki; Saddler, John N. ACS Sustainable Chemistry & Engineering, Vol. 6, Issue 5 https://doi.org/10.1021/acssuschemeng.8b00004	journal	April 2018
Bioethanol production from individual and mixed agricultural biomass residues Nguyen, Quynh Anh; Yang, Jianming; Bae, Hyeun-Jong Industrial Crops and Products, Vol. 95 https://doi.org/10.1016/j.indcrop.2016.11.040	journal	January 2017
Current advancement on the isolation, characterization and application of lignin Liao, Jing Jing; Latif, Nur Hanis Abd; Trache, Djalal International Journal of Biological Macromolecules, Vol. 162 https://doi.org/10.1016/j.ijbiomac.2020.06.168	journal	November 2020
Coordinated development of leading biomass pretreatment technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Bioresource Technology, Vol. 96, Issue 18, p. 1959-1966 https://doi.org/10.1016/j.biortech.2005.01.010	journal	December 2005
CHEMISTRY: Ionic Liquids--Solvents of the Future? Rogers, R. D.; Seddon, Kenneth R. Science, Vol. 302, Issue 5646, p. 792-793 https://doi.org/10.1126/science.1090313	journal	October 2003
The use of high-solids loadings in biomass pretreatment-a review Modenbach, Alicia A.; Nokes, Sue E. Biotechnology and Bioengineering, Vol. 109, Issue 6 https://doi.org/10.1002/bit.24464	journal	February 2012

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