Characterization of the Nannochloropsis gaditana storage carbohydrate: A 1,3-beta glucan with limited 1,6-branching

Vogler, Brian W.; Brannum, Jacob; Chung, Jeffrey W.; Seger, Mark; Posewitz, Matthew C.

doi:10.1016/j.algal.2018.10.011

Title: Characterization of the Nannochloropsis gaditana storage carbohydrate: A 1,3-beta glucan with limited 1,6-branching

Journal Article · Sat Oct 27 00:00:00 EDT 2018 · Algal Research

DOI:https://doi.org/10.1016/j.algal.2018.10.011· OSTI ID:1843734

Vogler, Brian W. ^[1]; Brannum, Jacob ^[1]; Chung, Jeffrey W. ^[2]; Seger, Mark ^[1]; Posewitz, Matthew C. ^[1]

Colorado School of Mines, Golden, CO (United States)
Univ. of Colorado School of Medicine, Denver, CO (United States)

As a result of a secondary endosymbiosis of a red alga, stramenopiles retained β-1,3 glucans as the primary soluble storage carbohydrate, in contrast to the starch and glycogen of green plants and animals. This storage carbohydrate has been identified and characterized in representative diatoms, brown algae, and oomycetes, but has not been biochemically characterized in species of Nannochloropsis, an industrially relevant genus of algae favored for its lipid content and productivity. In this study, the soluble storage carbohydrate of Nannochloropsis is characterized by ¹H NMR, linkage analysis, and size exclusion chromatography. The putative genes encoding enzymes required for synthesis of the carbohydrate oligosaccharide from glucose 6-phosphate are identified. A glycogenin-like glycosyltransferase family 8 (GT8) protein was also identified to be conserved among all species of Nannochloropsis, despite the lack of glycogen in the genus. Homologs were identified in available genomes of brown algae, diatoms, and oomycetes, all documented to utilize β-1,3 glucans for carbon storage. Finally, a set of three likely laminarinases are highlighted from the glycosyl hydrolases by phylogenetic analyses.

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Research Organization:: Colorado School of Mines, Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: EE0007089; SC0015662

OSTI ID:: 1843734

Alternate ID(s):: OSTI ID: 1637163; OSTI ID: 1839036

Journal Information:: Algal Research, Vol. 36; ISSN 2211-9264

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 14 works

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Title: Characterization of the Nannochloropsis gaditana storage carbohydrate: A 1,3-beta glucan with limited 1,6-branching

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