Identification and structural analysis of a thermophilic β-1,3-glucanase from compost

Feng, Jianwei; Xu, Shenyuan; Feng, Ruirui; Kovalevsky, Andrey; Zhang, Xia; Liu, Dongyang; Wan, Qun

doi:10.1186/s40643-021-00449-4

Title: Identification and structural analysis of a thermophilic β-1,3-glucanase from compost

Journal Article · Sun Oct 17 00:00:00 EDT 2021 · Bioresources and Bioprocessing

DOI:https://doi.org/10.1186/s40643-021-00449-4· OSTI ID:1830100

Feng, Jianwei ^[1]; Xu, Shenyuan ^[2]; Feng, Ruirui ^[1];

^[3]; Zhang, Xia ^[4]; Liu, Dongyang ^[1];

^[1]

Nanjing Agricultural University (China)
Zhejiang Univ. of Technology, Hangzhou (China). Key Laboratory of Bioorganic Synthesis of Zhejiang Province
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Qingdao Vland Biotech Group Inc., Qingdao (China)

β-1,3-glucanase can specifically hydrolyze glucans to oligosaccharides and has potential applications in biotechnology. We used the metatranscriptomic technology to discover a thermophilic β-1,3-glucanase from compost. The phylogenetic study shows that it belongs to the family 16 glycoside hydrolase (GH16) and is most homologous with an enzyme from Streptomyces sioyaensis, an actinobacterium. It has the activity of 146.9 U/mg in the optimal reaction condition (75 °C and pH 5.5). Its catalytic domain was crystallized and diffracted to 1.14 Å resolution. The crystal structure shows a sandwich-like β-jelly-roll fold with two disulfide bonds. After analyzing the occurring frequencies of these cysteine residues, we designed two mutants (C160G and C180I) to study the role of these disulfide bonds. Both mutants have decreased their optimal temperature from 75 to 70 °C, which indicate that the disulfide bonds are important to maintain thermostability. Interestingly, the activity of C160G has increased ~ 17% to reach 171.4 U/mg. We speculate that the increased activity of C160G mutant is due to increased dynamics near the active site. Our studies give a good example of balancing the rigidity and flexibility for enzyme activity, which is helpful for protein engineering.

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Research Organization:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1830100

Journal Information:: Bioresources and Bioprocessing, Vol. 8, Issue 1; ISSN 2197-4365

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Additional file 1 of Identification and structural analysis of a thermophilic β-1,3-glucanase from compost Feng, Jianwei; Xu, Shenyuan; Feng, Ruirui https://doi.org/10.6084/m9.figshare.16823695 The current record is supplemented by this text	text	January 2021
Identification and structural analysis of a thermophilic β-1,3-glucanase from compost Feng, Jianwei; Xu, Shenyuan; Feng, Ruirui https://doi.org/10.6084/m9.figshare.c.5666203 The current record is supplemented by this collection	collection	January 2021
Additional file 1 of Identification and structural analysis of a thermophilic β-1,3-glucanase from compost Feng, Jianwei; Xu, Shenyuan; Feng, Ruirui https://doi.org/10.6084/m9.figshare.16823695.v1 The current record is supplemented by this text	text	January 2021

Title: Identification and structural analysis of a thermophilic β-1,3-glucanase from compost

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