Imaging of Fibroblast Activation Protein Alpha Expression in a Preclinical Mouse Model of Glioma Using Positron Emission Tomography

Pandya, Darpan N.; Sinha, Akesh; Yuan, Hong; Mutkus, Lysette; Stumpf, Kristina; Marini, Frank C.; Wadas, Thaddeus J.

doi:10.3390/molecules25163672

Title: Imaging of Fibroblast Activation Protein Alpha Expression in a Preclinical Mouse Model of Glioma Using Positron Emission Tomography

Journal Article · Wed Aug 12 00:00:00 EDT 2020 · Molecules

DOI:https://doi.org/10.3390/molecules25163672· OSTI ID:1801852

Pandya, Darpan N. ^[1]; Sinha, Akesh ^[1]; Yuan, Hong ^[2];

^[3];

^[3]; Marini, Frank C. ^[3]; Wadas, Thaddeus J. ^[1]

Univ. of Iowa, Iowa City (United States). Dept. of Radiology
Univ. of North Carolina, Chapel Hill, NC (United States). Dept. of Radiology
Wake Forest Univ., Winston-Salem, NC (United States). Dept. of Regenerative Medicine

Glioblastoma multiforme (GBM) is the most aggressive glioma of the primary central nervous system. Due to the lack of effective treatment options, the prognosis for patients remains bleak. Fibroblast activation protein alpha (FAP), a 170 kDa type II transmembrane serine protease was observed to be expressed on glioma cells and within the glioma tumor microenvironment. To understand the utility of targeting FAP in this tumor type, the immuno-PET radiopharmaceutical [⁸⁹Zr]Zr-Df-Bz-F19 mAb was prepared and Lindmo analysis was used for its in vitro evaluation using the U87MG cell line, which expresses FAP endogenously. Lindmo analysis revealed an association constant (K_a) of 10^-8 M^-1 and an immunoreactivity of 52%. Biodistribution studies in U87MG tumor-bearing mice revealed increasing radiotracer retention in tumors over time, leading to average tumor-to-muscle ratios of 3.1, 7.3, 7.2, and 8.3 at 2, 24, 48 and 72 h, respectively. Small animal PET corroborated the biodistribution studies; tumor-to-muscle ratios at 2, 24, 48, and 72 h were 2.0, 5.0, 6.1 and 7.8, respectively. Autoradiography demonstrated accumulated activity throughout the interior of FAP⁺ tumors, while sequential tumor sections stained positively for FAP expression. Conversely, FAP^- tissues retained minimal radioactivity and were negative for FAP expression by immunohistochemistry. These results demonstrate FAP as a promising biomarker that may be exploited to diagnose and potentially treat GBM and other neuroepithelial cancers.

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Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0008657

OSTI ID:: 1801852

Journal Information:: Molecules, Vol. 25, Issue 16; ISSN 1420-3049

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Biochemistry & Molecular Biology
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Zirconium-89
fibroblast activation protein alpha
PET
cancer
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Title: Imaging of Fibroblast Activation Protein Alpha Expression in a Preclinical Mouse Model of Glioma Using Positron Emission Tomography

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