Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Geddes, Ryan D.; Wang, Xuan; Yomano, Lorraine P.; Miller, Elliot N.; Zheng, Huabao; Shanmugam, Keelnatham T.; Ingram, Lonnie O.

doi:10.1128/aem.01913-14

Title: Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

Journal Article · Tue Sep 09 00:00:00 EDT 2014 · Applied and Environmental Microbiology

DOI:https://doi.org/10.1128/aem.01913-14· OSTI ID:1771950

Geddes, Ryan D. ^[1]; Wang, Xuan ^[2]; Yomano, Lorraine P. ^[1]; Miller, Elliot N. ^[1]; Zheng, Huabao ^[1]; Shanmugam, Keelnatham T. ^[1]; Ingram, Lonnie O. ^[1]

Univ. of Florida, Gainesville, FL (United States)
Univ. of Florida, Gainesville, FL (United States); Arizona State Univ., Tempe, AZ (United States)

Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). Furthermore, this represents a new approach to increase furfural tolerance and may be useful for other organisms. Microarray comparisons of two furfural-resistant mutants (EMFR9 and EMFR35) provided initial evidence for the importance of polyamine transporters. Each mutant contained a single polyamine transporter gene that was upregulated over 100-fold (microarrays) compared to that in the parent LY180, as well as a mutation that silenced the expression of yqhD. Based on these genetic changes, furfural tolerance was substantially reconstructed in the parent, LY180.

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Research Organization:: Univ. of Florida, Gainesville, FL (United States)

Sponsoring Organization:: USDOE Office of International Affairs (IA)

Grant/Contract Number:: PI0000031

OSTI ID:: 1771950

Journal Information:: Applied and Environmental Microbiology, Vol. 80, Issue 19; ISSN 0099-2240

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
lignocellulose
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furfural
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polyamine
polyamine transporter

Title: Polyamine Transporters and Polyamines Increase Furfural Tolerance during Xylose Fermentation with Ethanologenic Escherichia coli Strain LY180

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