Integrin nanoclusters can bridge thin matrix fibres to form cell–matrix adhesions

Changede, Rishita; Cai, Haogang; Wind, Shalom J.; Sheetz, Michael P.

doi:10.1038/s41563-019-0460-y

Title: Integrin nanoclusters can bridge thin matrix fibres to form cell–matrix adhesions

Journal Article · Mon Sep 02 00:00:00 EDT 2019 · Nature Materials

DOI:https://doi.org/10.1038/s41563-019-0460-y· OSTI ID:1756877

^[1];

^[2];

^[3]; Sheetz, Michael P. ^[4]

National Univ. of Singapore (Singapore)
Columbia Univ., New York, NY (United States); Argonne National Lab. (ANL), Lemont, IL (United States)
Columbia Univ., New York, NY (United States)
National Univ. of Singapore (Singapore); Univ. of Texas Medical Branch, Galveston, TX (United States)

Integrin-mediated cell-matrix adhesions are key to sensing the geometry and rigidity of extracellular environments and influence vital cellular processes. In vivo, the extracellular matrix is composed of fibrous arrays. To understand the fibre geometries that are required for adhesion formation, we patterned nanolines of various line widths and arrangements in single, crossing or paired arrays with the integrin-binding peptide Arg-Gly-Asp. Single thin lines (width ≤ 30 nm) did not support cell spreading or formation of focal adhesions, despite the presence of a high density of Arg-Gly-Asp, but wide lines (>40 nm) did. Using super-resolution microscopy, we observed stable, dense integrin clusters formed on parallel (within 110 nm) or crossing thin lines (mimicking a matrix mesh) similar to those on continuous substrates. These dense clusters bridged the line pairs by recruiting activated but unliganded integrins, as verified by integrin mutants unable to bind ligands that coclustered with ligand-bound integrins when present in an active extended conformation. Furthermore, in a fibrous extracellular matrix mesh, stable integrin nano-clusters bridge between thin (≤ 30 nm) matrix fibres and bring about downstream consequences of cell motility and growth.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: National University of Singapore; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1756877

Journal Information:: Nature Materials, Vol. 18, Issue 12; ISSN 1476-1122

Publisher:: Springer Nature - Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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