The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum

Borowska-Zuchowska, Natalia; Kovarik, Ales; Robaszkiewicz, Ewa; Tuna, Metin; Tuna, Gulsemin Savas; Gordon, Sean; Vogel, John P; Hasterok, Robert

doi:10.1111/tpj.14869

Title: The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum

Journal Article · Fri Jul 03 00:00:00 EDT 2020 · The Plant Journal

DOI:https://doi.org/10.1111/tpj.14869· OSTI ID:1713233

Borowska-Zuchowska, Natalia ^[1]; Kovarik, Ales ^[2]; Robaszkiewicz, Ewa ^[1]; Tuna, Metin ^[3]; Tuna, Gulsemin Savas ^[4]; Gordon, Sean ^[5]; Vogel, John P ^[6]; Hasterok, Robert ^[1]

Univ. of Silesia in Katowice (Poland)
Academy of Sciences of the Czech Republic, Brno (Czech Republic)
Tekirdag Namik Kemal Univ., Tekirdag (Turkey)
Tekirdag Anatolian High School, Tekirdag (Turkey)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)

Nucleolar dominance (ND) consists of the reversible silencing of 35S/45S rDNA loci inherited from one of the ancestors of an allopolyploid. The molecular mechanisms by which one ancestral rDNA set is selected for silencing remain unclear. We applied a combination of molecular (Southern blot hybridization and reverse-transcription cleaved amplified polymorphic sequence analysis), genomic (analysis of variants) and cytogenetic (fluorescence in situ hybridization) approaches to study the structure, expression and epigenetic landscape of 35S rDNA in an allotetraploid grass that exhibits ND, Brachypodium hybridum (genome composition DDSS), and its putative progenitors, Brachypodium distachyon (DD) and Brachypodium stacei (SS). In progenitor genomes, B. stacei showed a higher intragenomic heterogeneity of rDNA compared with B. distachyon. In all studied accessions of B. hybridum, there was a reduction in the copy number of S homoeologues, which was accompanied by their inactive transcriptional status. The involvement of DNA methylation in CG and CHG contexts in the silencing of the S-genome rDNA loci was revealed. In the B. hybridum allotetraploid, ND is stabilized towards the D-genome units, irrespective of the polyphyletic origin of the species, and does not seem to be influenced by homoeologous 35S rDNA ratios and developmental stage.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Polish National Science Centre (NCN); Czech Science Foundation

Grant/Contract Number:: AC02-05CH11231; 2018/31/B/NZ3/01761; 2018/02/X/NZ3/03518; 20-14133J

OSTI ID:: 1713233

Journal Information:: The Plant Journal, Vol. 103, Issue 5; ISSN 0960-7412

Publisher:: Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
35S rDNA evolution
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allopolyploidy
Bracypodium hybridum
nucleolar dominance

Title: The fate of 35S rRNA genes in the allotetraploid grass Brachypodium hybridum

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