The Importance of Protein Phosphorylation for Signaling and Metabolism in Response to Diel Light Cycling and Nutrient Availability in a Marine Diatom

Tan, Maxine H.; Smith, Sarah R.; Hixson, Kim K.; Tan, Justin; McCarthy, James K.; Kustka, Adam B.; Allen, Andrew E.

doi:10.3390/biology9070155

Title: The Importance of Protein Phosphorylation for Signaling and Metabolism in Response to Diel Light Cycling and Nutrient Availability in a Marine Diatom

Journal Article · Mon Jul 06 00:00:00 EDT 2020 · Biology

DOI:https://doi.org/10.3390/biology9070155· OSTI ID:1673586

^[1];

^[2]; Hixson, Kim K. ^[3];

^[4]; McCarthy, James K. ^[2]; Kustka, Adam B. ^[5];

^[6]

Univ. of California, San Diego, CA (United States). Scripps Inst. of Oceanography
J. Craig Venter Inst., Inc., La Jolla, CA (United States). Microbial and Environmental Genomics
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab.
Univ. of California, San Diego, CA (United States). Dept. of Bioengineering
Rutgers Univ., New Brunswick, NJ (United States). Dept. of Earth and Environmental Sciences
Univ. of California, San Diego, CA (United States). Scripps Inst. of Oceanography; J. Craig Venter Inst., Inc., La Jolla, CA (United States). Microbial and Environmental Genomics

Diatoms are major contributors to global primary production and their populations in the modern oceans are affected by availability of iron, nitrogen, phosphate, silica, and other trace metals, vitamins, and infochemicals. However, little is known about the role of phosphorylation in diatoms and its role in regulation and signaling. We report a total of 2759 phosphorylation sites on 1502 proteins detected in Phaeodactylum tricornutum. Conditionally phosphorylated peptides were detected at low iron (n = 108), during the diel cycle (n = 149), and due to nitrogen availability (n = 137). Through a multi-omic comparison of transcript, protein, phosphorylation, and protein homology, we identify numerous proteins and key cellular processes that are likely under control of phospho-regulation. We show that phosphorylation regulates: (1) carbon retrenchment and reallocation during growth under low iron, (2) carbon flux towards lipid biosynthesis after the lights turn on, (3) coordination of transcription and translation over the diel cycle and (4) in response to nitrogen depletion. We also uncover phosphorylation sites for proteins that play major roles in diatom Fe sensing and utilization, including flavodoxin and phytotransferrin (ISIP2A), as well as identify phospho-regulated stress proteins and kinases. These findings provide much needed insight into the roles of protein phosphorylation in diel cycling and nutrient sensing in diatoms.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); Gordon and Betty Moore Foundation

Grant/Contract Number:: AC05-76RL01830; SC0006719; SC0008593; MCB-1024913; MCB-1818390; OCE-0727997; OCE-1756884; GBMF3828

OSTI ID:: 1673586

Report Number(s):: PNNL-SA-153917

Journal Information:: Biology, Vol. 9, Issue 7; ISSN 2079-7737

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
phosphoproteome
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diel
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