Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o -Methyltransferase Gene Family in Brachypodium distachyon

Wu, Xianting; Wu, Jiajie; Luo, Yangfan; Bragg, Jennifer; Anderson, Olin; Vogel, John; Gu, Yong Q.

doi:10.1155/2013/423189

Title: Phylogenetic, Molecular, and Biochemical Characterization of Caffeic Acid o -Methyltransferase Gene Family in Brachypodium distachyon

Journal Article · Tue Jan 01 00:00:00 EST 2013 · International Journal of Plant Genomics

DOI:https://doi.org/10.1155/2013/423189· OSTI ID:1629942

Wu, Xianting ^[1]; Wu, Jiajie ^[2]; Luo, Yangfan ^[3]; Bragg, Jennifer ^[3]; Anderson, Olin ^[3]; Vogel, John ^[3]; Gu, Yong Q. ^[3]

US Dept. of Agriculture (USDA), Albany, CA (United States). ARS-Central Great Plains Research Station. Western Regional Research Center; Univ. of California, Davis, CA (United States). Dept. of Plant Sciences
Shandong Agricultural Univ. (China). State Key Lab. of Crop Biology
US Dept. of Agriculture (USDA), Albany, CA (United States). ARS-Central Great Plains Research Station. Western Regional Research Center

Caffeic acido-methyltransferase (COMT) is one of the important enzymes controlling lignin monomer production in plant cell wall synthesis. Analysis of the genome sequence of the new grass modelBrachypodium distachyonidentified four COMT gene homologs, designated asBdCOMT1, BdCOMT2, BdCOMT3,andBdCOMT4. Phylogenetic analysis suggested that they belong to the COMT gene family, whereas syntenic analysis through comparisons with rice and sorghum revealed thatBdCOMT4on Chromosome 3 is the orthologous copy of the COMT genes well characterized in other grass species. The other three COMT genes are unique toBrachypodiumsince orthologous copies are not found in the collinear regions of rice and sorghum genomes. Expression studies indicated that all fourBrachypodiumCOMT genes are transcribed but with distinct patterns of tissue specificity. Full-length cDNAs were cloned in frame into the pQE-T7 expression vector for the purification of recombinantBrachypodiumCOMT proteins. Biochemical characterization of enzyme activity and substrate specificity showed that BdCOMT4 has significant effect on a broad range of substrates with the highest preference for caffeic acid. The other three COMTs had low or no effect on these substrates, suggesting that a diversified evolution occurred on these duplicate genes that not only impacted their pattern of expression, but also altered their biochemical properties.

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Research Organization:: Chicago Operations Office, Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

DOE Contract Number:: A102-07ER64452

OSTI ID:: 1629942

Journal Information:: International Journal of Plant Genomics, Vol. 2013; ISSN 1687-5370

Country of Publication:: United States

Language:: English

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