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Title: Aedes aegypti Odorant Binding Protein 22 selectively binds fatty acids through a conformational change in its C-terminal tail

Journal Article · · Scientific Reports
 [1];  [1];  [2];  [3]
  1. Univ. of Colorado, Aurora, CO (United States). School of Medicine. Anshutz Medical Campus. Dept. of Pharmacology
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Advanced Light Source (ALS). molecular Biology Consortium. Beamline 4.2.2
  3. Univ. of Colorado, Aurora, CO (United States). School of Medicine. Anshutz Medical Campus. Dept. of Pharmacology; Univ. of Colorado, Aurora, CO (United States). School of Medicine. Anshutz Medical Campus. Program in Structural Biology and Biochemistry
+ Show Author Affiliations

Aedes aegypti is the primary vector for transmission of Dengue, Zika and chikungunya viruses. Previously it was shown that Dengue virus infection of the mosquito led to an in increased expression of the odorant binding protein 22 (AeOBP22) within the mosquito salivary gland and that siRNA mediated knockdown of AeOBP22 led to reduced mosquito feeding behaviors. Insect OBPs are implicated in the perception, storage and transport of chemosensory signaling molecules including air-borne odorants and pheromones. AeOBP22 is unusual as it is additionally expressed in multiple tissues, including the antenna, the male reproductive glands and is transferred to females during reproduction, indicating multiple roles in the mosquito life cycle. However, it is unclear what role it plays in these tissues and what ligands it interacts with. Here we present solution and X-ray crystallographic studies that indicate a potential role of AeOBP22 binding to fatty acids, and that the specifcity for longer chain fatty acids is regulated by a conformational change in the C-terminal tail that leads to creation of an enlarged binding cavity that enhances binding afnity. This study sheds light onto the native ligands for AeOBP22 and provides insight into its potential functions in diferent tissues.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1629517
Journal Information:
Scientific Reports, Vol. 10, Issue 1; ISSN 2045-2322
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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