Detection of Epstein-Barr virus (EBV) in human lymphoma tissue by a novel microbial detection array

Journal Article · Fri Dec 05 00:00:00 EST 2014 · Biomarker Research

DOI:https://doi.org/10.1186/s40364-014-0024-x· OSTI ID:1629126

Tellez, Joseph ^[1]; Jaing, Crystal ^[2]; Wang, Jun ^[3]; Green, Ralph ^[1]; Chen, Mingyi ^[1]

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1. Univ. of California, Davis Medical Center, Davis, CA (United States). Dept. of Pathology and Lab. Medicine
2. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States). Biosciences and Biotechnology Division. Applied Genomics
3. Loma Lima Univ., Loma Linda, CA (United States). Dept. of Pathology and Lab. Medicine

Background: Infectious agents are estimated to play a causative role in approximately 20% of cancers worldwide. Viruses, notably the Epstein-Barr virus (EBV), are associated with 10-15% of B-cell lymphomas and are found at a higher frequency in immunosuppressed patients. In this study, we screened human lymphoma tissues using a novel Lawrence Livermore Microbial Detection Array (LLMDA), a comprehensive detection system that contains probes for all sequenced viruses and bacteria. This technology has been applied to identify pathogen-associated diseases. Results: We evaluated samples from 58 cases with various lymphoid tissue disorders using LLMDA. These included 30 B-cell lymphomas (9 indolent and 21 aggressive type), 2 T-cell lymphomas and 2 NK/T cell lymphomas, 4 plasmacytomas as well as 8 specimens of benign lymphoid tissue. Five of 21 high-grade B-cell lymphomas were positive for Epstein-Barr virus-encoded small RNA (EBER+), while all the indolent B-cell lymphomas were EBER-. Similarly, both NK/T cell lymphomas were EBER+, and the benign tissues were EBER-. We also screened 10 cases of post-transplant lymphoproliferative disorder (PTLD). Five of these cases (4 B-cell lymphomas and 1 NK/T cell lymphoma) were EBER+, and the remaining five cases were EBER-. Conclusions: We have confirmed the reliability of the LLMDA methods by detecting EBV in EBV-positive lymphomas while observing no false-positive results in EBV-negative lymphomas. The LLMDA technique provides a sensitive and alternative method for identifying known viral pathogen associated with tumors and may prove useful for future clinical identification of novel cancer-associated viral pathogens.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
• Grant/Contract Number: AC52-07NA27344
• OSTI ID: 1629126
• Journal Information: Biomarker Research, Vol. 2, Issue 1; ISSN 2050-7771
• Publisher: BioMed Central
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Epstein-Barr virus (EBV)
Lymphoma
Post-transplant lymphoproliferative disorder (PTLD)
Lawrence Livermore Microbial Detection Array (LLMDA)