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Title: Genome sequence of Ensifer sp. TW10; a Tephrosia wallichii (Biyani) microsymbiont native to the Indian Thar Desert

Journal Article · · Standards in Genomic Sciences
DOI:https://doi.org/10.4056/sigs.4598281· OSTI ID:1628681
 [1];  [1];  [1];  [1];  [2];  [2];  [2];  [3];  [4];  [5];  [5];  [5];  [6];  [5];  [5];  [5];  [6];  [5];  [5];  [2]
  1. Jai Narain Vyas Univ., Jodhpur (India). Dept. of Biology. BNF and Stress Biology Lab.
  2. Murdoch Univ., WA (Australia). Centre for Rhizobium Studies
  3. Deakin Univ., Melbourne, VIC (Australia). Faculty of Science, Engineering and Built Environment. School of Life and Environmental Sciences
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Bioscience Division
  5. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biologival Data Management and Technology Center
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Ensifer sp. TW10 is a novel N2 -fixing bacterium isolated from a root nodule of the perennial legume Tephrosia wallichii Graham (known locally as Biyani) found in the Great Indian (or Thar) desert, a large arid region in the northwestern part of the Indian subcontinent. Strain TW10 is a Gramnegative, rod shaped, aerobic, motile, non-spore forming, species of root nodule bacteria (RNB) that promiscuously nodulates legumes in Thar Desert alkaline soil. It is fast growing, acid-producing, and tolerates up to 2% NaCl and capable of growth at 40o C. In this report we describe for the first time the primary features of this Thar Desert soil saprophyte together with genome sequence information and annotation. The 6,802,256 bp genome has a GC content of 62% and is arranged into 57 scaffolds containing 6,470 protein-coding genes, 73 RNA genes and a single rRNA operon. This genome is one of 100 RNB genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.

Research Organization:
Los Alamos National Laboratory (LANL), Los Alamos, NM (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
Grant/Contract Number:
AC52-06NA25396; AC02-05CH11231; AC52-07NA27344
OSTI ID:
1628681
Journal Information:
Standards in Genomic Sciences, Vol. 9, Issue 2; ISSN 1944-3277
Publisher:
BioMed CentralCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (3)

Genomic characterization of Ensifer aridi, a proposed new species of nitrogen-fixing rhizobium recovered from Asian, African and American deserts journal January 2017
High-quality permanent draft genome sequence of Ensifer sp. PC2, isolated from a nitrogen-fixing root nodule of the legume tree (Khejri) native to the Thar Desert of India journal June 2016
Genome sequence of Ensifer medicae strain WSM1369; an effective microsymbiont of the annual legume Medicago sphaerocarpos journal December 2013

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Genetics & Heredity
Microbiology
root-nodule bacteria
nitrogen fixation
rhizobia
Alphaproteobacteria