Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation in Biological Systems

Weekley, Claire; Aitken, Jade; Finney, Lydia; Vogt, Stefan; Witting, Paul; Harris, Hugh

doi:10.3390/nu5051734

Title: Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation in Biological Systems

Journal Article · Wed May 01 00:00:00 EDT 2013 · Nutrients

DOI:https://doi.org/10.3390/nu5051734· OSTI ID:1628514

Weekley, Claire ^[1]; Aitken, Jade ^[2]; Finney, Lydia ^[3]; Vogt, Stefan ^[4]; Witting, Paul ^[2]; Harris, Hugh ^[1]

University of Adelaide, SA (Australia)
University of Sydney, NSW (Australia)
Argonne National Laboratory (ANL), Lemont, IL (United States)
Argonne National Lab. (ANL), Lemont, IL (United States)

Determining the speciation of selenium in vivo is crucial to understanding the biological activity of this essential element, which is a popular dietary supplement due to its anti-cancer properties. Hyphenated techniques that combine separation and detection methods are traditionally and effectively used in selenium speciation analysis, but require extensive sample preparation that may affect speciation. Synchrotron-based X-ray absorption and fluorescence techniques offer an alternative approach to selenium speciation analysis that requires minimal sample preparation. We present a brief summary of some key HPLC-ICP-MS and ESI-MS/MS studies of the speciation of selenium in cells and rat tissues. We review the results of a top-down approach to selenium speciation in human lung cancer cells that aims to link the speciation and distribution of selenium to its biological activity using a combination of X-ray absorption spectroscopy (XAS) and X-ray fluorescence microscopy (XFM). The results of this approach highlight the distinct fates of selenomethionine, methylselenocysteine and selenite in terms of their speciation and distribution within cells: organic selenium metabolites were widely distributed throughout the cells, whereas inorganic selenium metabolites were compartmentalized and associated with copper. New data from the XFM mapping of electrophoretically-separated cell lysates show the distribution of selenium in the proteins of selenomethionine-treated cells. Future applications of this top-down approach are discussed.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; Australian Government; Australian Research Council; Australian Synchrotron Postgraduate Award

Grant/Contract Number:: AC02-06CH11357; DP0985807

OSTI ID:: 1628514

Journal Information:: Nutrients, Vol. 5, Issue 5; ISSN 2072-6643

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

References (86)

Selenium Metabolites in Urine: A Critical Overview of Past Work and Current Status Francesconi, Kevin A.; Pannier, Florence Clinical Chemistry, Vol. 50, Issue 12 https://doi.org/10.1373/clinchem.2004.039875	journal	December 2004
X-ray-induced photo-chemistry and X-ray absorption spectroscopy of biological samples George, Graham N.; Pickering, Ingrid J.; Pushie, M. Jake Journal of Synchrotron Radiation, Vol. 19, Issue 6 https://doi.org/10.1107/S090904951203943X	journal	October 2012
Selenium and human health Ørskov, Hans; Flyvbjerg, Allan The Lancet, Vol. 356, Issue 9233 https://doi.org/10.1016/s0140-6736(05)73926-x	journal	September 2000
Selenium and human health Yusuf, S. Wamique The Lancet, Vol. 356, Issue 9233 https://doi.org/10.1016/s0140-6736(05)73929-5	journal	September 2000
Chemical alterations to murine brain tissue induced by formalin fixation: implications for biospectroscopic imaging and mapping studies of disease pathogenesis Hackett, Mark J.; McQuillan, James A.; El-Assaad, Fatima The Analyst, Vol. 136, Issue 14 https://doi.org/10.1039/c0an00269k	journal	January 2011
Element selective characterization of stability and reactivity of selenium species in selenized yeast Uden, Peter C.; Totoe Boakye, Harriet; Kahakachchi, Chethaka Journal of Analytical Atomic Spectrometry, Vol. 19, Issue 1 https://doi.org/10.1039/b307508g	journal	January 2004
In situ identification of dimethyl diselenide in hepatocytes treated with methylseleninic acid by membrane inlet mass spectrometry Gabel-Jensen, Charlotte; Bak, Søren A.; Lauritsen, Frants R. Journal of Analytical Atomic Spectrometry, Vol. 24, Issue 7 https://doi.org/10.1039/b903994e	journal	January 2009
Selenocysteine β-lyase and methylselenol demethylase in the metabolism of Se-methylated selenocompounds into selenide Suzuki, Kazuo T.; Kurasaki, Kazuki; Suzuki, Noriyuki Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1770, Issue 7 https://doi.org/10.1016/j.bbagen.2007.03.007	journal	July 2007
Time-dependent uptake, distribution and biotransformation of chromium(VI) in individual and bulk human lung cells: application of synchrotron radiation techniques Harris, Hugh H.; Levina, Aviva; Dillon, Carolyn T. JBIC Journal of Biological Inorganic Chemistry, Vol. 10, Issue 2 https://doi.org/10.1007/s00775-004-0617-1	journal	February 2005
Comparison of the chemical properties of selenocysteine and selenocystine with their sulfur analogs Huber, R. E.; Criddle, R. S. Archives of Biochemistry and Biophysics, Vol. 122, Issue 1 https://doi.org/10.1016/0003-9861(67)90136-1	journal	October 1967
Selenoproteins that function in cancer prevention and promotion Hatfield, Dolph L.; Yoo, Min-Hyuk; Carlson, Bradley A. Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1790, Issue 11 https://doi.org/10.1016/j.bbagen.2009.03.001	journal	November 2009
Selenium Inhibits Renal Oxidation and Inflammation But Not Acute Kidney Injury in an Animal Model of Rhabdomyolysis Shanu, Anu; Groebler, Ludwig; Kim, Hyun Bo Antioxidants & Redox Signaling, Vol. 18, Issue 7 https://doi.org/10.1089/ars.2012.4591	journal	March 2013
Trimethyl selenide. A urinary metabolite of selenite Byard, James L. Archives of Biochemistry and Biophysics, Vol. 130 https://doi.org/10.1016/0003-9861(69)90070-8	journal	January 1969
The human selenoproteome: recent insights into functions and regulation Reeves, M. A.; Hoffmann, P. R. Cellular and Molecular Life Sciences, Vol. 66, Issue 15 https://doi.org/10.1007/s00018-009-0032-4	journal	April 2009
Speciation of Selenoamino Acids and Organoselenium Compounds in Selenium-enriched Yeast Using High-performance Liquid Chromatography–Inductively Coupled Plasma Mass Spectrometry M. Bird, Susan; Uden, Peter C.; F. Tyson, Julian J. Anal. At. Spectrom., Vol. 12, Issue 7 https://doi.org/10.1039/a701429e	journal	January 1997
Availability and metabolism of 77Se-methylseleninic acid compared simultaneously with those of three related selenocompounds Suzuki, Kazuo T.; Ohta, Yuki; Suzuki, Noriyuki Toxicology and Applied Pharmacology, Vol. 217, Issue 1 https://doi.org/10.1016/j.taap.2006.07.005	journal	November 2006
Methylation and demethylation of intermediates selenide and methylselenol in the metabolism of selenium Ohta, Yuki; Suzuki, Kazuo T. Toxicology and Applied Pharmacology, Vol. 226, Issue 2 https://doi.org/10.1016/j.taap.2007.09.011	journal	January 2008
Uptake, Distribution, and Speciation of Selenoamino Acids by Human Cancer Cells: X-ray Absorption and Fluorescence Methods Weekley, Claire M.; Aitken, Jade B.; Vogt, Stefan Biochemistry, Vol. 50, Issue 10 https://doi.org/10.1021/bi101678a	journal	March 2011
Enzymic synthesis of selenocysteine in rat liver Esaki, Nobuyoshi; Nakamura, Takeshi; Tanaka, Hidehiko Biochemistry, Vol. 20, Issue 15 https://doi.org/10.1021/bi00518a039	journal	July 1981
Acid-volatile selenium formation catalyzed by glutathione reductase Hsieh, H. Steve; Ganther, Howard E. Biochemistry, Vol. 14, Issue 8 https://doi.org/10.1021/bi00679a014	journal	April 1975
Both Selenoproteins and Low Molecular Weight Selenocompounds Reduce Colon Cancer Risk in Mice with Genetically Impaired Selenoprotein Expression Irons, Robert; Carlson, Bradley A.; Hatfield, Dolph L. The Journal of Nutrition, Vol. 136, Issue 5 https://doi.org/10.1093/jn/136.5.1311	journal	May 2006
Methylselenocysteine Treatment Leads to Diselenide Formation in Human Cancer Cells: Evidence from X-ray Absorption Spectroscopy Studies Weekley, Claire M.; Aitken, Jade B.; Musgrave, Ian F. Biochemistry, Vol. 51, Issue 3 https://doi.org/10.1021/bi201462u	journal	January 2012
Influence of Dietary Methionine on the Metabolism of Selenomethionine in Rats Butler, J. A.; Beilstein, M. A.; Whanger, P. D. The Journal of Nutrition, Vol. 119, Issue 7 https://doi.org/10.1093/jn/119.7.1001	journal	July 1989
Selenium metabolism in rats with long-term ingestion of Se-methylselenocysteine using enriched stable isotopes Tsuji, Yoshiro; Suzuki, Noriyuki; T. Suzuki, Kazuo The Journal of Toxicological Sciences, Vol. 34, Issue 2 https://doi.org/10.2131/jts.34.191	journal	January 2009
Effects of selenium supplementation for cancer prevention in patients with carcinoma of the skin. Constant, Errikos Plastic & Reconstructive Surgery, Vol. 101, Issue 5 https://doi.org/10.1097/00006534-199804050-00069	journal	January 1998
Imaging Metals in Proteins by Combining Electrophoresis with Rapid X-ray Fluorescence Mapping Finney, Lydia; Chishti, Yasmin; Khare, Tripti ACS Chemical Biology, Vol. 5, Issue 6 https://doi.org/10.1021/cb1000263	journal	April 2010
Quantitative, chemically specific imaging of selenium transformation in plants Pickering, I. J.; Prince, R. C.; Salt, D. E. Proceedings of the National Academy of Sciences, Vol. 97, Issue 20 https://doi.org/10.1073/pnas.200244597	journal	September 2000
Re: Selenium and Vitamin E: Interesting Biology and Dashed Hope Goossens, Maria E.; Buntinx, Frank; Zeegers, Maurice P. JNCI: Journal of the National Cancer Institute, Vol. 101, Issue 19 https://doi.org/10.1093/jnci/djp272	journal	October 2009
Designing the Selenium and Vitamin E Cancer Prevention Trial (SELECT) Lippman, Scott M.; Goodman, Phyllis J.; Klein, Eric A. JNCI: Journal of the National Cancer Institute, Vol. 97, Issue 2 https://doi.org/10.1093/jnci/dji009	journal	January 2005
Speciation and Bioavailability of Selenium in Yeast-Based Intervention Agents Used in Cancer Chemoprevention Studies Larsen, Erik H.; Hansen, Marianne; Paulin, Helge Journal of AOAC INTERNATIONAL, Vol. 87, Issue 1 https://doi.org/10.1093/jaoac/87.1.225	journal	January 2004
Selenium metabolism in hepatocytes incubated with selenite, selenate, selenomethionine, Se-methylselenocysteine and methylseleninc acid and analysed by LC-ICP-MS Gabel-Jensen, Charlotte; Gammelgaard, Bente Journal of Analytical Atomic Spectrometry, Vol. 25, Issue 3 https://doi.org/10.1039/b921365a	journal	January 2010
An edge with XAS George, Graham N.; Hedman, Britt; Hodgson, Keith O. Nature Structural Biology, Vol. 5, Issue 8 https://doi.org/10.1038/1336	journal	August 1998
X-ray absorption spectroscopy of biological samples. A tutorial Ortega, Richard; Carmona, Asuncion; Llorens, Isabelle Journal of Analytical Atomic Spectrometry, Vol. 27, Issue 12 https://doi.org/10.1039/c2ja30224a	journal	January 2012
Selenocystine induces reactive oxygen species–mediated apoptosis in human cancer cells Chen, Tianfeng; Wong, Yum-Shing Biomedicine & Pharmacotherapy, Vol. 63, Issue 2 https://doi.org/10.1016/j.biopha.2008.03.009	journal	February 2009
Reduction of the selenotrisulfide derivative of glutathione to a persulfide analog by gluthathione reductase Ganther, Howard E. Biochemistry, Vol. 10, Issue 22 https://doi.org/10.1021/bi00798a013	journal	October 1971
Contribution of Enzymic α,γ-Elimination Reaction in Detoxification Pathway of Selenomethionine in Mouse Liver Okuno, Tomofumi; Kubota, Tomoyuki; Kuroda, Tomonori Toxicology and Applied Pharmacology, Vol. 176, Issue 1 https://doi.org/10.1006/taap.2001.9260	journal	October 2001
Selenomethionine Incorporation into Amyloid Sequences Regulates Fibrillogenesis and Toxicity Martínez, Javier; Lisa, Silvia; Sánchez, Rosa PLoS ONE, Vol. 6, Issue 11 https://doi.org/10.1371/journal.pone.0027999	journal	November 2011
Application of XANES spectroscopy in understanding the metabolism of selenium in isolated rainbow trout hepatocytes: insights into selenium toxicity Misra, Sougat; Peak, Derek; Niyogi, Som Metallomics, Vol. 2, Issue 10 https://doi.org/10.1039/c0mt00008f	journal	January 2010
Direct quantitative speciation of selenium in selenium-enriched yeast and yeast-based products by X-ray absorption spectroscopy confirmed by HPLC-ICP-MS Yu-Feng, Li; Xiaoyan, Wang; Liming, Wang Journal of Analytical Atomic Spectrometry, Vol. 25, Issue 3 https://doi.org/10.1039/b921570k	journal	January 2010
Chemical transformations of selenium in living organisms. Improved forms of selenium for cancer prevention Ganther, Howard E.; Robert Lawrence, J. Tetrahedron, Vol. 53, Issue 36 https://doi.org/10.1016/S0040-4020(97)00561-9	journal	August 1997
X-Ray Fluorescence Microscopy Reveals the Role of Selenium in Spermatogenesis Kehr, Sebastian; Malinouski, Mikalai; Finney, Lydia Journal of Molecular Biology, Vol. 389, Issue 5 https://doi.org/10.1016/j.jmb.2009.04.024	journal	June 2009
Selenium and Vitamin E: Interesting Biology and Dashed Hope Klein, E. A. JNCI Journal of the National Cancer Institute, Vol. 101, Issue 5 https://doi.org/10.1093/jnci/djp009	journal	February 2009
Complementary use of molecular and element-specific mass spectrometry for identification of selenium compounds related to human selenium metabolism Gammelgaard, Bente; Gabel-Jensen, Charlotte; Stürup, Stefan Analytical and Bioanalytical Chemistry, Vol. 390, Issue 7 https://doi.org/10.1007/s00216-007-1788-8	journal	January 2008
The Outcome of Selenium and Vitamin E Cancer Prevention Trial (SELECT) Reveals the Need for Better Understanding of Selenium Biology Hatfield, D. L.; Gladyshev, V. N. Molecular Interventions, Vol. 9, Issue 1 https://doi.org/10.1124/mi.9.1.6	journal	February 2009
Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function Jacob, Claus; Giles, Gregory I.; Giles, Niroshini M. Angewandte Chemie International Edition, Vol. 42, Issue 39, p. 4742-4758 https://doi.org/10.1002/anie.200300573	journal	October 2003
Localizing the Chemical Forms of Sulfur in Vivo Using X-ray Fluorescence Spectroscopic Imaging: Application to Onion ( Allium cepa ) Tissues Pickering, Ingrid J.; Sneeden, Eileen Yu; Prince, Roger C. Biochemistry, Vol. 48, Issue 29 https://doi.org/10.1021/bi900368x	journal	July 2009
Interactions of Cu(I) with Selenium-Containing Amino Acids Determined by NMR, XAS, and DFT Studies Wang, Hsiao C.; Riahi, Mindy; Pothen, Joshua Inorganic Chemistry, Vol. 50, Issue 21 https://doi.org/10.1021/ic201440j	journal	November 2011
Investigation of the selenium metabolism in cancer cell lines Lunøe, Kristoffer; Gabel-Jensen, Charlotte; Stürup, Stefan Metallomics, Vol. 3, Issue 2 https://doi.org/10.1039/c0mt00091d	journal	January 2011
Selenotrisulfides. Formation by the reaction of thiols with selenious acid Ganther, Howard E. Biochemistry, Vol. 7, Issue 8 https://doi.org/10.1021/bi00848a029	journal	August 1968
Selenium speciation analysis using inductively coupled plasma-mass spectrometry B’Hymer, C.; Caruso, J. A. Journal of Chromatography A, Vol. 1114, Issue 1 https://doi.org/10.1016/j.chroma.2006.02.063	journal	May 2006
Selenium and Vitamin E Supplementation for Cancer Prevention Rayman, Margaret P. JAMA, Vol. 301, Issue 18 https://doi.org/10.1001/jama.2009.625	journal	May 2009
Selenite is a substrate for calf thymus thioredoxin reductase and thioredoxin and elicits a large non-stoichiometric oxidation of NADPH in the presence of oxygen Kumar, Sushil; Bjornstedt, Mikael; Holmgren, Arne European Journal of Biochemistry, Vol. 207, Issue 2, p. 435-439 https://doi.org/10.1111/j.1432-1033.1992.tb17068.x	journal	July 1992
Separation and identification of selenotrisulfides in epithelial cell homogenates by LC–ICP–MS and LC–ESI-MS after incubation with selenite Gabel-Jensen, Charlotte; Gammelgaard, Bente; Bendahl, Lars Analytical and Bioanalytical Chemistry, Vol. 384, Issue 3 https://doi.org/10.1007/s00216-005-0178-3	journal	December 2005
Silver speciation in liver of marine mammals by synchrotron X-ray absorption fine structure and X-ray fluorescence spectroscopies Nakazawa, Emiko; Ikemoto, Tokutaka; Hokura, Akiko Journal of Environmental Monitoring, Vol. 13, Issue 6 https://doi.org/10.1039/c1em10115c	journal	January 2011
Selenium and human health Fryer, Michael J. The Lancet, Vol. 356, Issue 9233 https://doi.org/10.1016/s0140-6736(05)73928-3	journal	September 2000
Effects of Selenium Supplementation for Cancer Prevention in Patients With Carcinoma of the Skin: A Randomized Controlled Trial Clark, Larry C. JAMA, Vol. 276, Issue 24 https://doi.org/10.1001/jama.1996.03540240035027	journal	December 1996
Biological applications of X-ray fluorescence microscopy: exploring the subcellular topography and speciation of transition metals Fahrni, Christoph J. Current Opinion in Chemical Biology, Vol. 11, Issue 2 https://doi.org/10.1016/j.cbpa.2007.02.039	journal	April 2007
Both selenoproteins and low molecular weight selenocompounds reduce colon cancer risk in mice with genetically impaired selenoprotein expression Irons, Robert; Carlson, Bradley A.; Hatfield, Dolph L. The FASEB Journal, Vol. 20, Issue 4 https://doi.org/10.1096/fasebj.20.4.a428-d	journal	March 2006
Sodium selenite induces apoptosis by generation of superoxide via the mitochondrial-dependent pathway in human prostate cancer cells Xiang, Nong; Zhao, Rui; Zhong, Weixiong Cancer Chemotherapy and Pharmacology, Vol. 63, Issue 2 https://doi.org/10.1007/s00280-008-0745-3	journal	April 2008
State of the art report of selenium speciation in biological samples Połatajko, Aleksandra; Jakubowski, Norbert; Szpunar, Joanna J. Anal. At. Spectrom., Vol. 21, Issue 7 https://doi.org/10.1039/b605654g	journal	January 2006
Studies on the Biotransformations and Biodistributions of Metal-Containing Drugs Using X-Ray Absorption Spectroscopy B. Aitken, Jade; Levina, Aviva; A. Lay, Peter Current Topics in Medicinal Chemistry, Vol. 11, Issue 5 https://doi.org/10.2174/156802611794785217	journal	March 2011
Selenium and human health Rayman, Margaret P. The Lancet, Vol. 379, Issue 9822 https://doi.org/10.1016/S0140-6736(11)61452-9	journal	March 2012
Metabolism of 76Se-methylselenocysteine compared with that of 77Se-selenomethionine and 82Se-selenite Suzuki, Kazuo T.; Doi, Chiaki; Suzuki, Noriyuki Toxicology and Applied Pharmacology, Vol. 217, Issue 2 https://doi.org/10.1016/j.taap.2006.09.006	journal	December 2006
Selenocysteine lyase, a novel enzyme that specifically acts on selenocysteine. Mammalian distribution and purification and properties of pig liver enzyme. Esaki, N.; Nakamura, T.; Tanaka, H. Journal of Biological Chemistry, Vol. 257, Issue 8 https://doi.org/10.1016/S0021-9258(18)34734-3	journal	April 1982
Effect of Selenium and Vitamin E on Risk of Prostate Cancer and Other Cancers: The Selenium and Vitamin E Cancer Prevention Trial (SELECT) Lippman, Scott M.; Klein, Eric A.; Goodman, Phyllis J. JAMA, Vol. 301, Issue 1 https://doi.org/10.1001/jama.2008.864	journal	January 2009
Surveying selenium speciation from soil to cell—forms and transformations Gammelgaard, Bente; Jackson, Matthew I.; Gabel-Jensen, Charlotte Analytical and Bioanalytical Chemistry, Vol. 399, Issue 5 https://doi.org/10.1007/s00216-010-4212-8	journal	October 2010
Identifying metalloproteins through X-ray fluorescence mapping and mass spectrometry Raimunda, Daniel; Khare, Tripti; Giometti, Carol Metallomics, Vol. 4, Issue 9 https://doi.org/10.1039/c2mt20095c	journal	January 2012
Selenometabolomics: Identification of selenometabolites and specification of their biological significance by complementary use of elemental and molecular mass spectrometry Ogra, Yasumitsu; Anan, Yasumi Journal of Analytical Atomic Spectrometry, Vol. 24, Issue 11 https://doi.org/10.1039/b910235c	journal	January 2009
Comparison of Cupric and Sulfate Ion Effects on Chronic Selenosis in Rats Kezhou, Wei; Stowe, H. D.; House, A. M. Journal of Animal Science, Vol. 64, Issue 5 https://doi.org/10.2527/jas1987.6451467x	journal	May 1987
Excretion of Dimethyl Selenide by the rat McConnell, Kenneth P.; Portman, Oscar W. Journal of Biological Chemistry, Vol. 195, Issue 1 https://doi.org/10.1016/S0021-9258(19)50898-5	journal	March 1952
High-Resolution Imaging of Selenium in Kidneys: A Localized Selenium Pool Associated with Glutathione Peroxidase 3 Malinouski, Mikalai; Kehr, Sebastian; Finney, Lydia Antioxidants & Redox Signaling, Vol. 16, Issue 3 https://doi.org/10.1089/ars.2011.3997	journal	February 2012
Characterization, biological interactions and in-vivo detection of selenotrisulfide derivatives of glutathion, cysteine and homocysteine by HPLC-ICP-MS Braga, Paula; Montes-Bayón, María; Alvarez, Jesús J. Anal. At. Spectrom., Vol. 19, Issue 9 https://doi.org/10.1039/B402478H	journal	January 2004
The Chemical Nature of Mercury in Human Brain Following Poisoning or Environmental Exposure Korbas, Malgorzata; O’Donoghue, John L.; Watson, Gene E. ACS Chemical Neuroscience, Vol. 1, Issue 12 https://doi.org/10.1021/cn1000765	journal	October 2010
Selenium supplementation, baseline plasma selenium status and incidence of prostate cancer: an analysis of the complete treatment period of the Nutritional Prevention of Cancer Trial: SELENIUM SUPPLEMENTATION, PLASMA SELENIUM STATUS and PROSTATE CANCER Duffield-Lillico, A. J.; Dalkin, B. L.; Reid, M. E. BJU International, Vol. 91, Issue 7 https://doi.org/10.1046/j.1464-410X.2003.04167.x	journal	April 2003
Metabolism of Selenite in Human Lung Cancer Cells: X-Ray Absorption and Fluorescence Studies Weekley, Claire M.; Aitken, Jade B.; Vogt, Stefan Journal of the American Chemical Society, Vol. 133, Issue 45 https://doi.org/10.1021/ja206203c	journal	November 2011
Sulfur and Selenium: The Role of Oxidation State in Protein Structure and Function Jacob, Claus; Giles, Gregory I.; Giles, Niroshini M. ChemInform, Vol. 35, Issue 1 https://doi.org/10.1002/chin.200401269	journal	January 2004
Investigation of the selenium species distribution in a human B-cell lymphoma line by HPLC- and GC-ICP-MS in combination with HPLC-ESIMS/MS and GC-TOFMS after incubation with methylseleninic acid Goenaga Infante, Heidi; Joel, Simon P.; Warburton, Emma J. Anal. At. Spectrom., Vol. 22, Issue 8 https://doi.org/10.1039/b708620b	journal	January 2007
X-ray absorption spectroscopy of selenium-containing amino acids Pickering, I. J.; George, Graham N.; Van Fleet-Stalder, Verena JBIC Journal of Biological Inorganic Chemistry, Vol. 4, Issue 6 https://doi.org/10.1007/s007750050352	journal	December 1999
Methylseleninic acid, a potent growth inhibitor of synchronized mouse mammary epithelial tumor cells in vitro Sinha, Raghu; Unni, Emmanual; Ganther, Howard E. Biochemical Pharmacology, Vol. 61, Issue 3 https://doi.org/10.1016/s0006-2952(00)00545-1	journal	February 2001
Selenium supplementation for critical illness Kong, Zhengdong; Xia, Zhaofan The Lancet, Vol. 380, Issue 9840 https://doi.org/10.1016/s0140-6736(12)61287-2	journal	August 2012
Superoxide radical–initiated apoptotic signalling pathway in selenite-treated HepG2 cells: mitochondria serve as the main target Shen, Han-Ming; Yang, Cheng-Feng; Ding, Wen-Xing Free Radical Biology and Medicine, Vol. 30, Issue 1 https://doi.org/10.1016/s0891-5849(00)00421-4	journal	January 2001
Identification of a novel selenium metabolite, Se-methyl-N-acetylselenohexosamine, in rat urine by high-performance liquid chromatography–inductively coupled plasma mass spectrometry and –electrospray ionization tandem mass spectrometry Ogra, Yasumitsu; Ishiwata, Kazuya; Takayama, Hiromitsu Journal of Chromatography B, Vol. 767, Issue 2 https://doi.org/10.1016/s1570-0232(01)00581-5	journal	February 2002
Food-chain selenium and human health: spotlight on speciation Rayman, Margaret P.; Infante, Heidi Goenaga; Sargent, Mike British Journal of Nutrition, Vol. 100, Issue 2 https://doi.org/10.1017/s0007114508922522	journal	August 2008
Selenium compounds are substrates for glutaredoxins: a novel pathway for selenium metabolism and a potential mechanism for selenium-mediated cytotoxicity Wallenberg, Marita; Olm, Eric; Hebert, Christina Biochemical Journal, Vol. 429, Issue 1 https://doi.org/10.1042/bj20100368	journal	June 2010
Selenium in cancer prevention: a review of the evidence and mechanism of action Rayman, Margaret P. Proceedings of the Nutrition Society, Vol. 64, Issue 4 https://doi.org/10.1079/pns2005467	journal	November 2005
Dimethyldiselenide and Methylseleninic Acid Generate Superoxide in an In Vitro Chemiluminescence Assay in the Presence of Glutathione: Implications for the Anticarcinogenic Activity of L-Selenomethionine and L-Se-Methylselenocysteine Spallholz, Julian E.; Shriver, Brent J.; Reid, Ted W. Nutrition and Cancer, Vol. 40, Issue 1 https://doi.org/10.1207/s15327914nc401_8	journal	May 2001

Cited By (15)

Biological Selenium Species and Selenium Speciation in Biological Samples Bierla, Katarzyna; Szpunar, Joanna; Lobinski, Ryszard Selenium https://doi.org/10.1007/978-3-319-41283-2_35	book	September 2016
Extracellular Albumin Covalently Sequesters Selenocompounds and Determines Cytotoxicity Zheng, Wenyi; Boada, Roberto; He, Rui International Journal of Molecular Sciences, Vol. 20, Issue 19 https://doi.org/10.3390/ijms20194734	journal	September 2019
Which form is that? The importance of selenium speciation and metabolism in the prevention and treatment of disease Weekley, Claire M.; Harris, Hugh H. Chemical Society Reviews, Vol. 42, Issue 23 https://doi.org/10.1039/c3cs60272a	journal	January 2013
2014 Atomic Spectrometry Update – a review of advances in X-ray fluorescence spectrometry West, Margaret; Ellis, Andrew T.; Potts, Philip J. Journal of Analytical Atomic Spectrometry, Vol. 29, Issue 9 https://doi.org/10.1039/c4ja90038c	journal	January 2014
Selenium for preventing cancer Dennert, Gabriele; Zwahlen, Marcel; Brinkman, Maree https://doi.org/10.1002/14651858.cd005195.pub2	book	May 2011
Selenium for preventing cancer Vinceti, Marco; Filippini, Tommaso; Del Giovane, Cinzia Cochrane Database of Systematic Reviews https://doi.org/10.1002/14651858.cd005195.pub4	journal	January 2018
Selenium for preventing cancer. Vinceti, Marco; Filippini, Tommaso; Del Giovane, Cinzia WileyInterscience https://doi.org/10.7892/boris.110512	text	January 2018
ROS-modulated therapeutic approaches in cancer treatment Raza, Muhammad Hassan; Siraj, Sami; Arshad, Abida Journal of Cancer Research and Clinical Oncology, Vol. 143, Issue 9 https://doi.org/10.1007/s00432-017-2464-9	journal	June 2017
Element distribution and iron speciation in mature wheat grains ( Triticum aestivum L.) using synchrotron X-ray fluorescence microscopy mapping and X-ray absorption near-edge structure (XANES) imaging: Element distribution and iron speciation in wheat De Brier, Niels; Gomand, Sara V.; Donner, Erica Plant, Cell & Environment, Vol. 39, Issue 8 https://doi.org/10.1111/pce.12749	journal	June 2016
Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro Popović, Jelena; Klajn, Andrijana; Paunesku, Tatjana Cellular and Molecular Neurobiology, Vol. 39, Issue 5 https://doi.org/10.1007/s10571-019-00667-7	journal	March 2019
Intracellular distribution and stability of a luminescent rhenium( i ) tricarbonyl tetrazolato complex using epifluorescence microscopy in conjunction with X-ray fluorescence imaging Wedding, J. L.; Harris, H. H.; Bader, C. A. Metallomics, Vol. 9, Issue 4 https://doi.org/10.1039/c6mt00243a	journal	January 2017
Distribution and chemical form of selenium in Neptunia amplexicaulis from Central Queensland, Australia Harvey, Maggie-Anne; Erskine, Peter D.; Harris, Hugh H. Metallomics, Vol. 12, Issue 4 https://doi.org/10.1039/c9mt00244h	journal	January 2020
Friend or Foe? The Current Epidemiologic Evidence on Selenium and Human Cancer Risk Vinceti, Marco; Crespi, Catherine M.; Malagoli, Carlotta Journal of Environmental Science and Health, Part C, Vol. 31, Issue 4 https://doi.org/10.1080/10590501.2013.844757	journal	January 2013
Methylseleninic acid induces NAD(P)H:quinone oxidoreductase-1 expression through activation of NF-E2-related factor 2 in Chang liver cells Park, Jong-Min; Kim, Do-Hee; Na, Hye-Kyung Oncotarget, Vol. 9, Issue 3 https://doi.org/10.18632/oncotarget.10289	journal	June 2016
Metal and complementary molecular bioimaging in Alzheimer's disease Braidy, Nady; Poljak, Anne; Marjo, Christopher Frontiers in Aging Neuroscience, Vol. 6 https://doi.org/10.3389/fnagi.2014.00138	journal	July 2014

Similar Records

Investigation into the intracellular fates, speciation and mode of action of selenium-containing neuroprotective agents using XAS and XFM

Journal Article · Sat Apr 07 00:00:00 EDT 2018 · Biochimica et Biophysica Acta - General Subjects · OSTI ID:1628514

Wedding, Jason L.; Lai, Barry; Vogt, Stefan; +1 more

Exploring the structural basis for selenium/mercury antagonism in Allium fistulosum

Journal Article · Mon Dec 10 00:00:00 EST 2012 · Metallomics · OSTI ID:1628514

Afton, Scott E; Caruso, Joseph A

Selenium Accumulation, Distribution, and Speciation in Spineless Prickly Pear Cactus: A Drought- and Salt-Tolerant, Selenium-Enriched Nutraceutical Fruit Crop for Biofortified Foods

Journal Article · Fri Jul 01 00:00:00 EDT 2011 · Plant Physiology · OSTI ID:1628514

Banuelos, Gary S; Fakra, Sirine C; Walse, Spencer S; +5 more

Related Subjects

60 APPLIED LIFE SCIENCES
nutrition
dietetics
selenium
cancer
XAS
XFM
selenoproteins
SDS-PAGE

Title: Selenium Metabolism in Cancer Cells: The Combined Application of XAS and XFM Techniques to the Problem of Selenium Speciation in Biological Systems

Citation Formats

References (86)

Cited By (15)

Similar Records

Related Subjects