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Title: In Vitro Calcite Crystal Morphology Is Modulated by Otoconial Proteins Otolin-1 and Otoconin-90

Journal Article · · PLoS ONE
 [1];  [2];  [1];  [1];  [1];  [3];  [1]
  1. Washington University, St. Louis, MO (United States)
  2. George Washington University, Washington, DC (United States)
  3. Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). Molecular Foundry
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Otoconia are formed embryonically and are instrumental in detecting linear acceleration and gravity. Degeneration and fragmentation of otoconia in elderly patients leads to imbalance resulting in higher frequency of falls that are positively correlated with the incidence of bone fractures and death. In this work we investigate the roles otoconial proteins Otolin-1 and Otoconin 90 (OC90) perform in the formation of otoconia. We demonstrate by rotary shadowing and atomic force microscopy (AFM) experiments that Otolin-1 forms homomeric protein complexes and self-assembled networks supporting the hypothesis that Otolin-1 serves as a scaffold protein of otoconia. Our calcium carbonate crystal growth data demonstrate that Otolin-1 and OC90 modulate in vitro calcite crystal morphology but neither protein is sufficient to produce the shape of otoconia. Coadministration of these proteins produces synergistic effects on crystal morphology that contribute to morphology resembling otoconia.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
Grant/Contract Number:
AC02-05CH11231; R01 DC011614; R21 DC009320
OSTI ID:
1627693
Journal Information:
PLoS ONE, Vol. 9, Issue 4; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (4)

Chiral switching in biomineral suprastructures induced by homochiral l-amino acid journal August 2018
Zebrafish Otolith Biomineralization Requires Polyketide Synthase posted_content January 2018
Effect of calcium ions on structure and stability of the C1q-like domain of otolin-1 from human and zebrafish journal November 2017
Mechanisms of otoconia and otolith development: MECHANISMS OF OTOCONIA AND OTOLITH DEVELOPMENT journal October 2014

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
calcite
crystal growth
crystals
atomic force microscopy
mica
collagens
recombinant proteins
biomineralization