The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle

Erbilgin, Onur; Sutter, Markus; Kerfeld, Cheryl A.

doi:10.1371/journal.pbio.1002399

Title: The Structural Basis of Coenzyme A Recycling in a Bacterial Organelle

Journal Article · Wed Mar 09 00:00:00 EST 2016 · PLoS Biology (Online)

DOI:https://doi.org/10.1371/journal.pbio.1002399· OSTI ID:1627173

Erbilgin, Onur ^[1]; Sutter, Markus ^[2]; Kerfeld, Cheryl A. ^[3]

Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology. MSU-DOE Plant Research Lab.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division
Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; Michigan State Univ., East Lansing, MI (United States). Dept. of Biochemistry and Molecular Biology. MSU-DOE Plant Research Lab.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Molecular Biophysics and Integrated Bioimaging Division

Bacterial Microcompartments (BMCs) are proteinaceous organelles that encapsulate critical segments of autotrophic and heterotrophic metabolic pathways; they are functionally diverse and are found across 23 different phyla. The majority of catabolic BMCs (metabolosomes) compartmentalize a common core of enzymes to metabolize compounds via a toxic and/or volatile aldehyde intermediate. The core enzyme phosphotransacylase (PTAC) recycles Coenzyme A and generates an acyl phosphate that can serve as an energy source. The PTAC predominantly associated with metabolosomes (PduL) has no sequence homology to the PTAC ubiquitous among fermentative bacteria (Pta). Here, we report two high-resolution PduL crystal structures with bound substrates. The PduL fold is unrelated to that of Pta; it contains a dimetal active site involved in a catalytic mechanism distinct from that of the housekeeping PTAC. Accordingly, PduL and Pta exemplify functional, but not structural, convergent evolution. The PduL structure, in the context of the catalytic core, completes our understanding of the structural basis of cofactor recycling in the metabolosome lumen.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1627173

Journal Information:: PLoS Biology (Online), Vol. 14, Issue 3; ISSN 1545-7885

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

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