Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny

Zhao, You-jie; Liu, Xin-yi; Guo, Ran; Hu, Kun-rong; Cao, Yong; Dai, Fei

doi:10.1186/s12864-019-5627-z

Title: Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny

Journal Article · Fri Mar 29 00:00:00 EDT 2019 · BMC Genomics

DOI:https://doi.org/10.1186/s12864-019-5627-z· OSTI ID:1626821

Zhao, You-jie ^[1]; Liu, Xin-yi ^[2]; Guo, Ran ^[2]; Hu, Kun-rong ^[2]; Cao, Yong ^[2]; Dai, Fei ^[2]

Southwest Forestry Univ., Yunnan (China). Key Lab. of Forestry and Ecological Big Data State Forestry Administration; Southwest Forestry Univ., Yunnan (China). College of Big data and Intelligent Engineering; Southwest Forestry Univ., Yunnan (China). Ministry of Education. Key Lab. for Forest Resources Conservation and Utilization in the Southwest Mountains of China
Southwest Forestry Univ., Yunnan (China). College of Big data and Intelligent Engineering

Background: Willows are widely distributed in the northern hemisphere and have good adaptability to different living environment. The increasing of genome and transcriptome data provides a chance for comparative analysis to study the evolution patterns with the different origin and geographical distributions in the Salix phylogeny. Results: Transcript sequences of 10 Salicaceae species were downloaded from public databases. All pairwise of orthologues were identified by comparative analysis in these species, from which we constructed a phylogenetic tree and estimated the rate of diverse. Divergence times were estimated in the 10 Salicaceae using comparative transcriptomic analysis. All of the fast-evolving positive selection sequences were identified, and some cold-, drought-, light-, universal-, and heat- resistance genes were discovered. Conclusions: The divergence time of subgenus Vetrix and Salix was about 17.6–16.0 Mya during the period of Middle Miocene Climate Transition (21–14 Mya). Subgenus Vetrix diverged to migratory and resident groups when the climate changed to the cool and dry trend by 14 Mya. Cold- and light- stress genes were involved in positive selection among the resident Vetrix, and which would help them to adapt the cooling stage. Universal- stress genes exhibited positive selection among the migratory group and subgenus Salix. These data are useful for comprehending the adaptive evolution and speciation in the Salix lineage.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1626821

Journal Information:: BMC Genomics, Vol. 20, Issue 1; ISSN 1471-2164

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

References (61)

Transcriptome characterisation of Pinus tabuliformis and evolution of genes in the Pinus phylogeny Niu, Shi-Hui; Li, Zhe-Xin; Yuan, Hu-Wei BMC Genomics, Vol. 14, Issue 1 https://doi.org/10.1186/1471-2164-14-263	journal	January 2013
A Phylogenetic Analysis of Salix (Salicaceae) Based on matK and Ribosomal DNA Sequence Data Hardig, T. M.; Anttila, C. K.; Brunsfeld, S. J. Journal of Botany, Vol. 2010 https://doi.org/10.1155/2010/197696	journal	December 2010
Transcriptomic Response of Chinese Yew (Taxus chinensis) to Cold Stress Meng, Delong; Yu, Xianghua; Ma, Liyuan Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.00468	journal	April 2017
Evidence for rapid climate change in the Mesozoic–Palaeogene greenhouse world Jenkyns, Hugh C. Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences, Vol. 361, Issue 1810 https://doi.org/10.1098/rsta.2003.1240	journal	July 2003
Transcriptomic Response of Purple Willow (Salix purpurea) to Arsenic Stress Yanitch, Aymeric; Brereton, Nicholas J. B.; Gonzalez, Emmanuel Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01115	journal	June 2017
Transcriptome comparison reveals the patterns of selection in domesticated and wild ramie (Boehmeria nivea L. Gaud) Liu, Touming; Tang, Shouwei; Zhu, Siyuan Plant Molecular Biology, Vol. 86, Issue 1-2 https://doi.org/10.1007/s11103-014-0214-9	journal	June 2014
Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences Li, W.; Godzik, A. Bioinformatics, Vol. 22, Issue 13 https://doi.org/10.1093/bioinformatics/btl158	journal	May 2006
Clustal W and Clustal X version 2.0 Larkin, M. A.; Blackshields, G.; Brown, N. P. Bioinformatics, Vol. 23, Issue 21, p. 2947-2948 https://doi.org/10.1093/bioinformatics/btm404	journal	September 2007
Selection of Conserved Blocks from Multiple Alignments for Their Use in Phylogenetic Analysis Castresana, J. Molecular Biology and Evolution, Vol. 17, Issue 4 https://doi.org/10.1093/oxfordjournals.molbev.a026334	journal	April 2000
High-density linkage mapping and evolution of paralogs and orthologs in Salix and Populus Berlin, Sofia; Lagercrantz, Ulf; von Arnold, Sara BMC Genomics, Vol. 11, Issue 1 https://doi.org/10.1186/1471-2164-11-129	journal	January 2010
Cold regulation of plastid ascorbate peroxidases serves as a priming hub controlling ROS signaling in Arabidopsis thaliana van Buer, Jörn; Cvetkovic, Jelena; Baier, Margarete BMC Plant Biology, Vol. 16, Issue 1 https://doi.org/10.1186/s12870-016-0856-7	journal	July 2016
Trends, Rhythms, and Aberrations in Global Climate 65 Ma to Present Zachos, J. Science, Vol. 292, Issue 5517 https://doi.org/10.1126/science.1059412	journal	April 2001
Comparative transcriptomics reveals patterns of selection in domesticated and wild tomato Koenig, D.; Jimenez-Gomez, J. M.; Kimura, S. Proceedings of the National Academy of Sciences, Vol. 110, Issue 28 https://doi.org/10.1073/pnas.1309606110	journal	June 2013
Plastome phylogeny and lineage diversification of Salicaceae with focus on poplars and willows Zhang, Lei; Xi, Zhenxiang; Wang, Mingcheng Ecology and Evolution, Vol. 8, Issue 16 https://doi.org/10.1002/ece3.4261	journal	July 2018
De novo transcriptome assembly, development of EST-SSR markers and population genetic analyses for the desert biomass willow, Salix psammophila Jia, Huixia; Yang, Haifeng; Sun, Pei Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep39591	journal	December 2016
The Miocene Climatic Optimum: evidence from ectothermic vertebrates of Central Europe Böhme, Madelaine Palaeogeography, Palaeoclimatology, Palaeoecology, Vol. 195, Issue 3-4 https://doi.org/10.1016/S0031-0182(03)00367-5	journal	June 2003
Structural and functional insight into the universal stress protein family Tkaczuk, Karolina L.; A. Shumilin, Igor; Chruszcz, Maksymilian Evolutionary Applications, Vol. 6, Issue 3 https://doi.org/10.1111/eva.12057	journal	March 2013
Phylogenetic Relationships of American Willows (Salix L., Salicaceae) Lauron-Moreau, Aurélien; Pitre, Frédéric E.; Argus, George W. PLOS ONE, Vol. 10, Issue 4 https://doi.org/10.1371/journal.pone.0121965	journal	April 2015
An extinct genus of Salicaceae based on twigs with attached flowers, fruits, and foliage from the Eocene Green River Formation of Utah and Colorado, USA Boucher, Lisa D.; Manchester, Steven R.; Judd, Walter S. American Journal of Botany, Vol. 90, Issue 9 https://doi.org/10.3732/ajb.90.9.1389	journal	September 2003
Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants Deng, Shurong; Sun, Jian; Zhao, Rui Plant Physiology, Vol. 169, Issue 1 https://doi.org/10.1104/pp.15.00581	journal	July 2015
Blast2GO: a universal tool for annotation, visualization and analysis in functional genomics research Conesa, A.; Gotz, S.; Garcia-Gomez, J. M. Bioinformatics, Vol. 21, Issue 18 https://doi.org/10.1093/bioinformatics/bti610	journal	August 2005
Full-length transcriptome assembly from RNA-Seq data without a reference genome Grabherr, Manfred G.; Haas, Brian J.; Yassour, Moran Nature Biotechnology, Vol. 29, Issue 7 https://doi.org/10.1038/nbt.1883	journal	May 2011
Phylogeny of Salix subgenus Salix s.l. (Salicaceae): delimitation, biogeography, and reticulate evolution Wu, Jie; Nyman, Tommi; Wang, Dong-Chao BMC Evolutionary Biology, Vol. 15, Issue 1 https://doi.org/10.1186/s12862-015-0311-7	journal	March 2015
Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family Heddad, M.; Adamska, I. Proceedings of the National Academy of Sciences, Vol. 97, Issue 7 https://doi.org/10.1073/pnas.97.7.3741	journal	March 2000
Comparative Transcriptomic Approaches Exploring Contamination Stress Tolerance in Salix sp. Reveal the Importance for a Metaorganismal de Novo Assembly Approach for Nonmodel Plants Brereton, Nicholas J. B.; Gonzalez, Emmanuel; Marleau, Julie Plant Physiology, Vol. 171, Issue 1 https://doi.org/10.1104/pp.16.00090	journal	May 2016
Widespread Paleopolyploidy in Model Plant Species Inferred from Age Distributions of Duplicate Genes Blanc, Guillaume; Wolfe, Kenneth H. The Plant Cell, Vol. 16, Issue 7 https://doi.org/10.1105/tpc.021345	journal	June 2004
OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes Li, L. Genome Research, Vol. 13, Issue 9 https://doi.org/10.1101/gr.1224503	journal	September 2003
Wind to insect pollination ratios and floral traits in five alpine Salix species Peeters, Liza; Totland, Ørjan Canadian Journal of Botany, Vol. 77, Issue 4 https://doi.org/10.1139/b99-003	journal	September 1999
The willow genome and divergent evolution from poplar after the common genome duplication Dai, Xiaogang; Hu, Quanjun; Cai, Qingle Cell Research, Vol. 24, Issue 10 https://doi.org/10.1038/cr.2014.83	journal	July 2014
High temperatures in the terrestrial mid-latitudes during the early Palaeogene Naafs, B. D. A.; Rohrssen, M.; Inglis, G. N. Nature Geoscience, Vol. 11, Issue 10 https://doi.org/10.1038/s41561-018-0199-0	journal	July 2018
The Genome of Black Cottonwood, Populus trichocarpa (Torr. & Gray) Tuskan, G. A.; DiFazio, S.; Jansson, S. Science, Vol. 313, Issue 5793, p. 1596-1604 https://doi.org/10.1126/science.1128691	journal	September 2006
Phylogenomic Analysis and Dynamic Evolution of Chloroplast Genomes in Salicaceae Huang, Yuan; Wang, Jun; Yang, Yongping Frontiers in Plant Science, Vol. 8 https://doi.org/10.3389/fpls.2017.01050	journal	June 2017
Overexpression of quinone reductase from Salix matsudana Koidz enhances salt tolerance in transgenic Arabidopsis thaliana Song, Xixi; Fang, Jie; Han, Xiaojiao Gene, Vol. 576, Issue 1 https://doi.org/10.1016/j.gene.2015.10.069	journal	January 2016
Glutathione S-transferase Mu 2-transduced mesenchymal stem cells ameliorated anti-glomerular basement membrane antibody-induced glomerulonephritis by inhibiting oxidation and inflammation Li, Yajuan; Yan, Mei; Yang, Jichen Stem Cell Research & Therapy, Vol. 5, Issue 1 https://doi.org/10.1186/scrt408	journal	January 2014
The Role of Universal Stress Proteins in Bacterial Infections O’Connor, Andrew; McClean, Siobhán Current Medicinal Chemistry, Vol. 24, Issue 36 https://doi.org/10.2174/0929867324666170124145543	journal	November 2017
Phylogenetic relationships of Salix (Salicaceae) based on rbc L sequence data Azuma, Takayuki; Kajita, Tadashi; Yokoyama, Jun American Journal of Botany, Vol. 87, Issue 1 https://doi.org/10.2307/2656686	journal	January 2000
Foliage and Fruits of Early Poplars (Salicaceae: Populus ) from the Eocene of Utah, Colorado, and Wyoming Manchester, Steven R.; Judd, Walter S.; Handley, Bruce International Journal of Plant Sciences, Vol. 167, Issue 4 https://doi.org/10.1086/503918	journal	July 2006
Climate model sensitivity to changes in Miocene paleotopography Herold, N.; You, Y.; Müller, R. D. Australian Journal of Earth Sciences, Vol. 56, Issue 8 https://doi.org/10.1080/08120090903246170	journal	December 2009
Molecular phylogeny of Salix L. (Salicaceae) inferred from three chloroplast datasets and its systematic implications Chen, Jia-Hui; Sun, Hang; Wen, Jun TAXON, Vol. 59, Issue 1 https://doi.org/10.1002/tax.591004	journal	February 2010
Transcriptome Analysis of the Differentially Expressed Genes in the Male and Female Shrub Willows (Salix suchowensis) Liu, Jingjing; Yin, Tongming; Ye, Ning PLoS ONE, Vol. 8, Issue 4 https://doi.org/10.1371/journal.pone.0060181	journal	April 2013
Cross Protection by Cold-shock to Salinity and Drought Stress-induced Oxidative Stress in Mustard (Brassica campestris L.) Seedlings Hossain, Mohammad Anwar; Mostofa, Mohammad Golam; Fujita, Masayuki Molecular Plant Breeding https://doi.org/10.5376/mpb.2013.04.0007	journal	January 2013
De Novo Transcriptome and Small RNA Analysis of Two Chinese Willow Cultivars Reveals Stress Response Genes in Salix matsudana Rao, Guodong; Sui, Jinkai; Zeng, Yanfei PLoS ONE, Vol. 9, Issue 10 https://doi.org/10.1371/journal.pone.0109122	journal	October 2014
MUSCLE: multiple sequence alignment with high accuracy and high throughput Edgar, R. C. Nucleic Acids Research, Vol. 32, Issue 5, p. 1792-1797 https://doi.org/10.1093/nar/gkh340	journal	March 2004
The genome portal of the Department of Energy Joint Genome Institute: 2014 updates Nordberg, Henrik; Cantor, Michael; Dusheyko, Serge Nucleic Acids Research, Vol. 42, Issue D1 https://doi.org/10.1093/nar/gkt1069	journal	November 2013
Pre-Miocene birth of the Yangtze River Zheng, H.; Clift, P. D.; Wang, P. Proceedings of the National Academy of Sciences, Vol. 110, Issue 19 https://doi.org/10.1073/pnas.1216241110	journal	April 2013
PAML 4: Phylogenetic Analysis by Maximum Likelihood Yang, Z. Molecular Biology and Evolution, Vol. 24, Issue 8 https://doi.org/10.1093/molbev/msm088	journal	April 2007
Transcriptome sequencing of Pinus kesiya var. langbianensis and comparative analysis in the Pinus phylogeny Zhao, You-jie; Cao, Yong; Wang, Juan BMC Genomics, Vol. 19, Issue 1 https://doi.org/10.1186/s12864-018-5127-6	journal	October 2018
Southern high-latitude terrestrial climate change during the Palaeocene–Eocene derived from a marine pollen record (ODP Site 1172, East Tasman Plateau) Contreras, L.; Pross, J.; Bijl, P. K. Climate of the Past, Vol. 10, Issue 4 https://doi.org/10.5194/cp-10-1401-2014	journal	January 2014
CD-HIT Suite: a web server for clustering and comparing biological sequences Huang, Ying; Niu, Beifang; Gao, Ying Bioinformatics, Vol. 26, Issue 5 https://doi.org/10.1093/bioinformatics/btq003	journal	January 2010
MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0 Tamura, Koichiro; Stecher, Glen; Peterson, Daniel Molecular Biology and Evolution, Vol. 30, Issue 12 https://doi.org/10.1093/molbev/mst197	journal	October 2013
Role of NSD1 in H2O2-induced GSTM3 suppression Chu, Guoming; Li, Yinghui; Dong, Xiaolong Cellular Signalling, Vol. 26, Issue 12 https://doi.org/10.1016/j.cellsig.2014.08.026	journal	December 2014
Global vegetation dynamics and latitudinal temperature gradients during the Mid to Late Miocene (15.97–5.33Ma) Pound, Matthew J.; Haywood, Alan M.; Salzmann, Ulrich Earth-Science Reviews, Vol. 112, Issue 1-2 https://doi.org/10.1016/j.earscirev.2012.02.005	journal	April 2012
Universal Stress Proteins in Escherichia coli Siegele, Deborah A. Journal of Bacteriology, Vol. 187, Issue 18 https://doi.org/10.1128/JB.187.18.6253-6254.2005	journal	September 2005
Southern high-latitude terrestrial climate change during the Paleocene–Eocene derived from a marine pollen record (ODP Site 1172, East Tasman Plateau) Contreras, L.; Pross, J.; Bijl, P. K. Climate of the Past Discussions, Vol. 10, Issue 1 https://doi.org/10.5194/cpd-10-291-2014	journal	January 2014
Antiquity and fundamental processes of the antler cycle in Cervidae (Mammalia) Rössner, Gertrud E.; Costeur, Loïc; Scheyer, Torsten M. The Science of Nature, Vol. 108, Issue 1 https://doi.org/10.1007/s00114-020-01713-x	journal	December 2020
Light stress-regulated two-helix proteins in Arabidopsis thaliana related to the chlorophyll a/b-binding gene family Heddad, M. Proceedings of the National Academy of Sciences, Vol. 97, Issue 7 https://doi.org/10.1073/pnas.050391397	journal	March 2000
Genome Sequencing of Kocuria varians Strain 80, Isolated from Starter Culture for Dry Sausage Konorov, Evgenii A.; Kurbakov, Konstantin A.; Minaev, Mikhail Y. Microbiology Resource Announcements, Vol. 10, Issue 8 https://doi.org/10.1128/mra.00086-21	journal	February 2021
Complete Genome Sequence of Clostridium botulinum CJ0611A1, a Type A(B) Isolate Associated with an International Outbreak of Botulism from Commercial Carrot Juice Harris, Richard; Dussault, Forest; Flint, Annika Microbiology Resource Announcements, Vol. 10, Issue 1 https://doi.org/10.1128/mra.01111-20	journal	January 2021
Wind to insect pollination ratios and floral traits in five alpine Salix species Peeters, Liza; Totland, Ørjan Canadian Journal of Botany, Vol. 77, Issue 4 https://doi.org/10.1139/cjb-77-4-556	journal	January 1999
Emiliania huxleyi endures N-limitation with an efficient metabolic budgeting and effective ATP synthesis Rokitta, Sebastian D.; Von Dassow, Peter; Rost, Björn BMC Genomics, Vol. 15, Issue 1 https://doi.org/10.1186/1471-2164-15-1051	journal	January 2014
Consistency between molecular phylogeny and morphological classification of the Salix matsudana Koidz. complex (Salicaceae) Du, S. H.; Wang, Z. S.; Li, Y. X. Genetics and Molecular Research, Vol. 14, Issue 3 https://doi.org/10.4238/2015.july.31.15	journal	January 2015

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59 BASIC BIOLOGICAL SCIENCES
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Additional file 1: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927604 The current record is supplemented by this dataset	dataset	January 2019
Additional file 1: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927604.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 2: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927607 The current record is supplemented by this image	image	January 2019
Additional file 2: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927607.v1 The current record is supplemented by this image	image	January 2019
Additional file 3: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927616 The current record is supplemented by this dataset	dataset	January 2019
Additional file 3: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927616.v1 The current record is supplemented by this dataset	dataset	January 2019
Additional file 4: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927622 The current record is supplemented by this image	image	January 2019
Additional file 4: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927622.v1 The current record is supplemented by this image	image	January 2019
Additional file 5: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927628 The current record is supplemented by this dataset	dataset	January 2019
Additional file 5: of Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.7927628.v1 The current record is supplemented by this dataset	dataset	January 2019
Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.c.4453649 The current record is supplemented by this collection	collection	January 2019
Comparative genomics and transcriptomics analysis reveals evolution patterns of selection in the Salix phylogeny Zhao, You-Jie; Liu, Xin-Yi; Guo, Ran https://doi.org/10.6084/m9.figshare.c.4453649.v1 The current record is supplemented by this collection	collection	January 2019