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Title: Functional interaction of chloroplast SRP/FtsY with the ALB3 translocase in thylakoids

Journal Article · · Journal of Cell Biology
 [1];  [2];  [3];  [1]
  1. Univ. of Arkansas, Fayetteville, AR (United States). Dept. of Biological Sciences
  2. Univ. of Arkansas, Fayetteville, AR (United States). Dept. of Biological Sciences; Univ. of Arkansas, Fayetteville, AR (United States). Dept. of Chemistry and Biochemistry
  3. Okayama Univ., Okayama (Japan). Graduate School of Natural Science and Technology
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Integration of thylakoid proteins by the chloroplast signal recognition particle (cpSRP) posttranslational transport pathway requires the cpSRP, an SRP receptor homologue (cpFtsY), and the membrane protein ALB3. Similarly, Escherichia coli uses an SRP and FtsY to cotranslationally target membrane proteins to the SecYEG translocase, which contains an ALB3 homologue, YidC. In neither system are the interactions between soluble and membrane components well understood. We show that complexes containing cpSRP, cpFtsY, and ALB3 can be precipitated using affinity tags on cpSRP or cpFtsY. Stabilization of this complex with GMP-PNP specifically blocks subsequent integration of substrate (light harvesting chl a/b-binding protein [LHCP]), indicating that the complex occupies functional ALB3 translocation sites. Surprisingly, neither substrate nor cpSRP43, a component of cpSRP, was necessary to form a complex with ALB3. Complexes also contained cpSecY, but its removal did not inhibit ALB3 function. Furthermore, antibody bound to ALB3 prevented ALB3 association with cpSRP and cpFtsY and inhibited LHCP integration suggesting that a complex containing cpSRP, cpFtsY, and ALB3 must form for proper LHCP integration.

Research Organization:
Univ. of Arkansas, Fayetteville, AR (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division; National Institutes of Health (NIH)
Grant/Contract Number:
FG02-01ER15161; 1 P20 RR15569-02
OSTI ID:
1625132
Journal Information:
Journal of Cell Biology, Vol. 162, Issue 7; ISSN 0021-9525
Publisher:
Rockefeller University PressCopyright Statement
Country of Publication:
United States
Language:
English

References (40)

YidC, an assembly site for polytopic Escherichia coli membrane proteins located in immediate proximity to the SecYE translocon and lipids journal August 2001
Erratum to: “Toc, Tic, and chloroplast protein import” [Biochim. Biophys. Acta 1541 (2001) 64–79] journal June 2002
Loss of Albino3 Leads to the Specific Depletion of the Light-Harvesting System journal September 2002
An Imported Thylakoid Protein Accumulates in the Stroma When Insertion into Thylakoids is Inhibited journal August 1989
Protein Import and Routing Systems of Chloroplasts journal April 1999
Distinct “Assisted” and “Spontaneous” Mechanisms for the Insertion of Polytopic Chlorophyll-binding Proteins into the Thylakoid Membrane journal February 1999
Evidence for a Stromal GTP Requirement for the Integration of a Chlorophyll a/b-Binding Polypeptide into Thylakoid Membranes journal May 1994
YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase journal February 2000
SecDFyajC forms a heterotetrameric complex with YidC: SecDFyajC-YidC complex formation journal May 2002
Chloroplast FtsY, Chloroplast Signal Recognition Particle, and GTP Are Required to Reconstitute the Soluble Phase of Light-harvesting Chlorophyll Protein Transport into Thylakoid Membranes journal September 1999
Signal Sequence Recognition and Protein Targeting to the Endoplasmic Reticulum Membrane journal November 1994
The NG domain of the prokaryotic signal recognition particle receptor, FtsY, is fully functional when fused to an unrelated integral membrane polypeptide journal June 1997
A chloroplast homologue of the signal recognition particle subunit SRP54 is involved in the posttranslational integration of a protein into thylakoid membranes. journal April 1995
Thylakoid Δ pH-dependent precursor proteins bind to a cpTatC–Hcf106 complex before Tha4-dependent transport journal August 2001
A novel signal recognition particle targets light-harvesting proteins to the thylakoid membranes journal August 1998
Empty Site Forms of the SRP54 and SRα GTPases Mediate Targeting of Ribosome–Nascent Chain Complexes to the Endoplasmic Reticulum journal May 1997
The thylakoid membrane protein ALB3 associates with the cpSecY-translocase in Arabidopsis thaliana journal December 2002
Protein Transport Across the Eukaryotic Endoplasmic Reticulum and Bacterial Inner Membranes journal June 1996
Chloroplast Oxa1p Homolog Albino3 Is Required for Post-translational Integration of the Light Harvesting Chlorophyll-binding Protein into Thylakoid Membranes journal January 2000
Protein-specific energy requirements for protein transport across or into thylakoid membranes. Two lumenal proteins are transported in the absence of ATP. journal February 1992
YidC/Oxa1p/Alb3: evolutionarily conserved mediators of membrane protein assembly journal July 2001
Interaction of E. coli Ffh/4.5S ribonucleoprotein and FtsY mimics that of mammalian signal recognition particle and its receptor journal February 1994
YidC mediates membrane protein insertion in bacteria journal August 2000
SRbeta coordinates signal sequence release from SRP with ribosome binding to the translocon journal May 2001
Making membranes in bacteria journal August 2000
Requirement of GTP hydrolysis for dissociation of the signal recognition particle from its receptor journal May 1991
Synthesis, membrane insertion and assembly of the chloroplast-encoded D1 protein into photosystem II journal January 2002
Transient interaction of cpSRP54 with elongating nascent chains of the chloroplast-encoded D1 protein; ‘cpSRP54 caught in the act’ journal July 2002
Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids journal March 1999
Two distinct mechanisms for the translocation of proteins across the thylakoid membrane, one requiring the presence of a stromal protein factor and nucleotide triphosphates. journal February 1994
Switching the Model: A Concerted Mechanism for GTPases in Protein Targeting journal May 1997
Toc, Tic, and chloroplast protein import journal December 2001
Post-translational protein translocation into thylakoids by the Sec and ΔpH-dependent pathways journal December 2001
Function of a chloroplast SRP in thylakoid protein export journal December 2001
Functional Characterization of Recombinant Chloroplast Signal Recognition Particle journal May 2001
Distinct Albino3-dependent and -independent Pathways for Thylakoid Membrane Protein Insertion journal November 2001
The Oxa1 Protein Forms a Homooligomeric Complex and Is an Essential Part of the Mitochondrial Export Translocase in Neurospora crassa journal April 2002
ATP Stimulates Signal Recognition Particle (SRP)/FtsY-supported Protein Integration in Chloroplasts journal August 2002
Component Specificity for the Thylakoidal Sec and Delta Ph–Dependent Protein Transport Pathways journal July 1999
YidC, a Newly Defined Evolutionarily Conserved Protein, Mediates Membrane Protein Assembly in Bacteria journal January 2002

Cited By (16)

YidC/Alb3/Oxa1 Family of Insertases journal June 2015
Deletion of the chloroplast LTD protein impedes LHCI import and PSI–LHCI assembly in Chlamydomonas reinhardtii journal December 2017
Chloroplast PetD protein: evidence for SRP/Alb3-dependent insertion into the thylakoid membrane journal November 2017
Organization of chlorophyll biosynthesis and insertion of chlorophyll into the chlorophyll-binding proteins in chloroplasts journal May 2015
Functional Update of the Auxiliary Proteins PsbW, PsbY, HCF136, PsbN, TerC and ALB3 in Maintenance and Assembly of PSII journal April 2016
Membrane binding of the bacterial signal recognition particle receptor involves two distinct binding sites journal August 2006
Ribosome nascent chain complexes of the chloroplast-encoded cytochrome b6 thylakoid membrane protein interact with cpSRP54 but not with cpSecY journal January 2015
Molecular mechanism of SRP-dependent light-harvesting protein transport to the thylakoid membrane in plants journal June 2018
Inserting membrane proteins: The YidC/Oxa1/Alb3 machinery in bacteria, mitochondria, and chloroplasts journal March 2011
Structural basis for cpSRP43 chromodomain selectivity and dynamics in Alb3 insertase interaction journal November 2015
Complementation of a mutation in CpSRP43 causing partial truncation of light-harvesting chlorophyll antenna in Chlorella vulgaris journal December 2017
F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis journal April 2004
YidC family members are involved in the membrane insertion, lateral integration, folding, and assembly of membrane proteins journal September 2004
Promiscuous targeting of polytopic membrane proteins to SecYEG or YidC by theEscherichia colisignal recognition particle journal February 2012
The Chloroplast SRP Systems of Chaetosphaeridium globosum and Physcomitrella patens as Intermediates in the Evolution of SRP-Dependent Protein Transport in Higher Plants journal November 2016
Evolution of YidC/Oxa1/Alb3 insertases: three independent gene duplications followed by functional specialization in bacteria, mitochondria and chloroplasts journal February 2011

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