Boxwood blight: an ongoing threat to ornamental and native boxwood

LeBlanc, Nicholas; Salgado-Salazar, Catalina; Crouch, Jo Anne

doi:10.1007/s00253-018-8936-2

Title: Boxwood blight: an ongoing threat to ornamental and native boxwood

Journal Article · Mon Apr 02 00:00:00 EDT 2018 · Applied Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s00253-018-8936-2· OSTI ID:1623598

^[1]; Salgado-Salazar, Catalina ^[1]; Crouch, Jo Anne ^[2]

US Dept. of Agriculture (USDA), Beltsville, MD (United States). Agricultural Research Service; Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)
US Dept. of Agriculture (USDA), Beltsville, MD (United States). Agricultural Research Service

Boxwood blight is an emerging disease of ornamental and native boxwood plants in the family Buxaceae. First documented in the 1990s at a single location in England, the disease is now reported throughout Europe, Asia, New Zealand, and North America. To address the growing concern over boxwood blight, ongoing research focuses on multiple biological and genetic aspects of the causal pathogens and susceptible host plants. Characterization of genetic variation among the Calonectria fungi that cause boxwood blight shows that two unique sister species with different geographic distributions incite the disease. Studies of the pathogen life cycle show the formation of long-lived survival structures and that host infection is dependent on inoculum density, temperature, and humidity. Host range investigations detail high levels of susceptibility among boxwood as well as the potential for asymptomatic boxwood infection and for other plants in the family Buxaceae to serve as additional hosts. Multiple DNA-based diagnostic assays are available, ranging from probe-based quantitative PCR assays to the use of comparative genomics to develop robust diagnostic markers or provide whole genome-scale identifications. Though many questions remain, the research that continues to address boxwood blight demonstrates the importance of applying a multidisciplinary approach to understand and control emerging plant diseases.

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Research Organization:: Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE; USDA; Agricultural Research Service (ARS)

Grant/Contract Number:: AC05-06OR23100

OSTI ID:: 1623598

Journal Information:: Applied Microbiology and Biotechnology, Vol. 102, Issue 10; ISSN 0175-7598

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 28 works

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References (63)

First report of box blight caused by Cylindrocladium pseudonaviculatum in the Eastern Black Sea region of Turkey Akilli, S.; Katircioglu, Y. Z.; Zor, K. New Disease Reports, Vol. 25 https://doi.org/10.5197/j.2044-0588.2012.025.023	journal	April 2012
Effects of Inoculum Dose, Temperature, Cultivar, and Interrupted Leaf Wetness Period on Infection of Boxwood by Calonectria pseudonaviculata Avenot, H. F.; King, C.; Edwards, T. P. Plant Disease, Vol. 101, Issue 6 https://doi.org/10.1094/PDIS-05-16-0742-RE	journal	June 2017
Widespread selective sweeps throughout the genome of model plant pathogenic fungi and identification of effector candidates Badouin, H.; Gladieux, P.; Gouzy, J. Molecular Ecology, Vol. 26, Issue 7 https://doi.org/10.1111/mec.13976	journal	January 2017
Genome datasets for Calonectria henricotiae and C. pseudonaviculata causing boxwood blight disease and related fungal species Crouch, Jo Anne; Malapi-Wight, Martha; Rivera, Yazmín Ag Data Commons https://doi.org/10.15482/USDA.ADC/1410184	dataset	January 2017
An Improved Leaf Disc Bioassay for Detecting Calonectria pseudonaviculata in Soil and Potting Media Dart, Norm; Hong, Chuanxue; Bradley, William Tyler Plant Disease, Vol. 98, Issue 12 https://doi.org/10.1094/PDIS-03-14-0233-RE	journal	December 2014
Soil Inoculum Production, Survival, and Infectivity of the Boxwood Blight Pathogen, Calonectria pseudonaviculata Dart, Norm; Hong, Chuanxue; Craig, Caryn Allen Plant Disease, Vol. 99, Issue 12 https://doi.org/10.1094/PDIS-12-14-1245-RE	journal	December 2015
Buxus in Europe: Late Quaternary dynamics and modern vulnerability Di Domenico, Francesco; Lucchese, Fernando; Magri, Donatella Perspectives in Plant Ecology, Evolution and Systematics, Vol. 14, Issue 5 https://doi.org/10.1016/j.ppees.2012.07.001	journal	October 2012
qPCR Assays for the Detection of Cylindrocladium buxicola in Plant, Water, and Air Samples Gehesquière, B.; D'Haeyer, S.; Pham, K. T. K. Plant Disease, Vol. 97, Issue 8 https://doi.org/10.1094/PDIS-10-12-0964-RE	journal	August 2013
Characterization and taxonomic reassessment of the box blight pathogen Calonectria pseudonaviculata , introducing Calonectria henricotiae sp. nov. Gehesquière, B.; Crouch, J. A.; Marra, R. E. Plant Pathology, Vol. 65, Issue 1 https://doi.org/10.1111/ppa.12401	journal	May 2015
Drivers of emerging fungal diseases of forest trees Ghelardini, Luisa; Pepori, Alessia Lucia; Luchi, Nicola Forest Ecology and Management, Vol. 381 https://doi.org/10.1016/j.foreco.2016.09.032	journal	December 2016
Microbiome Selection Could Spur Next-Generation Plant Breeding Strategies Gopal, Murali; Gupta, Alka Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.01971	journal	December 2016
First report of box blight caused by Cylindrocladium buxicola in Georgia Gorgiladze, L.; Meparishvili, G.; Sikharulidze, Z. New Disease Reports, Vol. 23 https://doi.org/10.5197/j.2044-0588.2011.023.024	journal	April 2011
Effective Bioassays for Evaluating Boxwood Blight Susceptibility Using Detached Stem Inoculations Guo, Yonghong; Olsen, Richard T.; Kramer, Matthew HortScience, Vol. 50, Issue 2 https://doi.org/10.21273/HORTSCI.50.2.268	journal	February 2015
Use of Mycelium and Detached Leaves in Bioassays for Assessing Resistance to Boxwood Blight Guo, Yonghong; Olsen, Richard T.; Kramer, Matthew Plant Disease, Vol. 100, Issue 8 https://doi.org/10.1094/PDIS-01-16-0016-RE	journal	August 2016
Cylindrocladium buxicola , a new species affecting Buxus spp., and its phylogenetic status Henricot, Béatrice; Culham, Alastair Mycologia, Vol. 94, Issue 6 https://doi.org/10.1080/15572536.2003.11833155	journal	November 2002
A new blight disease on Buxus in the UK caused by the fungus Cylindrocladium Henricot, B.; Perez Sierra, A.; Prior, C. Plant Pathology, Vol. 49, Issue 6 https://doi.org/10.1046/j.1365-3059.2000.00508.x	journal	December 2000
Studies on the Control of Cylindrocladium buxicola Using Fungicides and Host Resistance Henricot, B.; Gorton, C.; Denton, G. Plant Disease, Vol. 92, Issue 9 https://doi.org/10.1094/PDIS-92-9-1273	journal	September 2008
(2085) Proposal to conserve the name Cylindrocladium buxicola against C. pseudonaviculatum ( Ascomycota ) Henricot, Béatrice; David, John; Ivors, Kelly TAXON, Vol. 61, Issue 5 https://doi.org/10.1002/tax.615021	journal	October 2012
First Report of Boxwood Blight Caused by Calonectria pseudonaviculata in Florida Iriarte, Fanny; Paret, Mathews; Knox, Gary Plant Health Progress, Vol. 17, Issue 4 https://doi.org/10.1094/PHP-BR-16-0027	journal	January 2016
First Report of Boxwood Blight Caused by Cylindrocladium pseudonaviculatum in the United States Ivors, K. L.; Lacey, L. W.; Milks, D. C. Plant Disease, Vol. 96, Issue 7 https://doi.org/10.1094/PDIS-03-12-0247-PDN	journal	July 2012
Biocontrol of boxwood blight by Trichoderma koningiopsis Mb2 Kong, Ping; Hong, Chuanxue Crop Protection, Vol. 98 https://doi.org/10.1016/j.cropro.2017.03.015	journal	August 2017
First Report of Blight of Sarcococca hookeriana var. humilis by Calonectria pseudonaviculata in Virginia Kong, P.; Likins, T. M.; Hong, C. X. Plant Disease, Vol. 101, Issue 1 https://doi.org/10.1094/PDIS-05-16-0654-PDN	journal	January 2017
First Report of Pachysandra terminalis Leaf Spot by Calonectria pseudonaviculata in Virginia Kong, P.; Likins, T. M.; Hong, C. X. Plant Disease, Vol. 101, Issue 3 https://doi.org/10.1094/PDIS-10-16-1513-PDN	journal	March 2017
Calonectria pseudonaviculata can Cause Leaf Spot and Stem Blight of Pachysandra procumbens LaMondia, J. A.; Li, D. W. Plant Health Progress, Vol. 14, Issue 1 https://doi.org/10.1094/PHP-2013-0226-01-BR	journal	January 2013
First Report of Cylindrocladium pseudonaviculatum Causing Leaf Spot of Pachysandra terminalis LaMondia, J. A.; Li, D. W.; Marra, R. E. Plant Disease, Vol. 96, Issue 7 https://doi.org/10.1094/PDIS-03-12-0235-PDN	journal	July 2012
Species concepts in Calonectria (Cylindrocladium) Lombard, L.; Crous, P. W.; Wingfield, B. D. Studies in Mycology, Vol. 66 https://doi.org/10.3114/sim.2010.66.01	journal	January 2010
LAMP Detection Assays for Boxwood Blight Pathogens: A Comparative Genomics Approach Malapi-Wight, Martha; Demers, Jill E.; Veltri, Daniel Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep26140	journal	May 2016
Sarcococca Blight: Use of Whole-Genome Sequencing for Fungal Plant Disease Diagnosis Malapi-Wight, Martha; Salgado-Salazar, Catalina; Demers, Jill E. Plant Disease, Vol. 100, Issue 6 https://doi.org/10.1094/PDIS-10-15-1159-RE	journal	June 2016
Effect of initial darkness duration on the pathogenicity of Calonectria pseudonaviculata on boxwood Marine, S. C.; Baudoin, A.; Hong, C. X. Plant Pathology, Vol. 67, Issue 3 https://doi.org/10.1111/ppa.12754	journal	September 2017
Report of the Nomenclature Committee for Fungi — 20 May, Tom W. IMA Fungus, Vol. 8, Issue 1 https://doi.org/10.5598/imafungus.2017.08.01.12	journal	June 2017
Influence of resistance breeding in common bean on rhizosphere microbiome composition and function Mendes, Lucas William; Raaijmakers, Jos M.; de Hollander, Mattias The ISME Journal, Vol. 12, Issue 1 https://doi.org/10.1038/ismej.2017.158	journal	October 2017
Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation Mori, Yasuyoshi; Nagamine, Kentaro; Tomita, Norihiro Biochemical and Biophysical Research Communications, Vol. 289, Issue 1 https://doi.org/10.1006/bbrc.2001.5921	journal	November 2001
Plant integrity: An important factor in plant-pathogen interactions Orłowska, Elżbieta; Llorente, Briardo; Cvitanich, Cristina Plant Signaling & Behavior, Vol. 8, Issue 1 https://doi.org/10.4161/psb.22513	journal	January 2013
Boxwood Blight: A New Scourge, a New Paradigm for Collaborative Research Palmer, Cristi L. Outlooks on Pest Management, Vol. 25, Issue 3 https://doi.org/10.1564/v25_jun_10	journal	June 2014
First Report of Cylindrocladium buxicola on Buxus sempervirens in Spain Varela, C. Pintos; Penalta, B. González; Vázquez, J. P. Mansilla Plant Disease, Vol. 93, Issue 6 https://doi.org/10.1094/PDIS-93-6-0670B	journal	June 2009
First report of Cylindrocladium buxicola on box in the Czech Republic Šafránková, I.; Kmoch, M.; Holková, L. New Disease Reports, Vol. 25 https://doi.org/10.5197/j.2044-0588.2012.025.005	journal	February 2012
First Report of Blight Disease on Buxus Caused by Cylindrocladium buxicola in France Saurat, C.; Fourrier, C.; Ioos, R. Plant Disease, Vol. 96, Issue 7 https://doi.org/10.1094/PDIS-03-12-0242-PDN	journal	July 2012
The Effect of Different Temperatures and Moisture Levels on Survival of Calonectria pseudonaviculata in Boxwood Leaves and Twigs and as Microsclerotia Produced in Culture Shishkoff, Nina; Camp, Mary J. Plant Disease, Vol. 100, Issue 10 https://doi.org/10.1094/PDIS-09-15-1098-RE	journal	October 2016
Evaluating Boxwood Susceptibility to Calonectria pseudonaviculata Using Cuttings from the National Boxwood Collection Shishkoff, Nina; Daughtrey, Margery; Aker, Scott Plant Health Progress, Vol. 16, Issue 1 https://doi.org/10.1094/PHP-RS-14-0033	journal	January 2015
Fast and specific detection of the invasive forest pathogen Heterobasidion irregulare through a Loop-mediated isothermal AMPlification (LAMP) assay Sillo, F.; Giordano, L.; Gonthier, P. Forest Pathology, Vol. 48, Issue 2 https://doi.org/10.1111/efp.12396	journal	October 2017
Development of Polymorphic Genic-SSR Markers by cDNA Library Sequencing in Boxwood, Buxus spp. (Buxaceae) Thammina, Chandra S.; Olsen, Richard T.; Malapi-Wight, Martha Applications in Plant Sciences, Vol. 2, Issue 12 https://doi.org/10.3732/apps.1400095	journal	December 2014
Genetic relationships of boxwood (Buxus L.) accessions based on genic simple sequence repeat markers Thammina, Chandra S.; Olsen, Richard T.; Kramer, Matthew Genetic Resources and Crop Evolution, Vol. 64, Issue 6 https://doi.org/10.1007/s10722-016-0436-6	journal	September 2016
Current knowledge on plant/canopy architectural traits that reduce the expression and development of epidemics Tivoli, Bernard; Calonnec, Agnès; Richard, Benjamin European Journal of Plant Pathology, Vol. 135, Issue 3 https://doi.org/10.1007/s10658-012-0066-6	journal	September 2012
Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products Tomita, Norihiro; Mori, Yasuyoshi; Kanda, Hidetoshi Nature Protocols, Vol. 3, Issue 5 https://doi.org/10.1038/nprot.2008.57	journal	April 2008
Genetic relationships in European and Asiatic Buxus species based on AFLP markers, genome sizes and chromosome numbers Van Laere, Katrijn; Hermans, Didier; Leus, Leen Plant Systematics and Evolution, Vol. 293, Issue 1-4 https://doi.org/10.1007/s00606-011-0422-6	journal	March 2011
Effectors as Tools in Disease Resistance Breeding Against Biotrophic, Hemibiotrophic, and Necrotrophic Plant Pathogens Vleeshouwers, Vivianne G. A. A.; Oliver, Richard P. Molecular Plant-Microbe Interactions®, Vol. 27, Issue 3 https://doi.org/10.1094/MPMI-10-13-0313-IA	journal	March 2014
First Report of Boxwood Blight Caused by Calonectria pseudonaviculata in Kentucky Ward Gauthier, N. A.; Amsden, B.; Beale, J. Plant Disease, Vol. 100, Issue 5 https://doi.org/10.1094/PDIS-10-15-1162-PDN	journal	May 2016
Histological Evidence that Microsclerotia Play a Significant Role in Disease Cycle of the Boxwood Blight Pathogen in Southeastern United States and Implications for Disease Mitigation Weeda, Sarah M.; Dart, Norman L. Plant Health Progress, Vol. 13, Issue 1 https://doi.org/10.1094/PHP-2012-0403-01-BR	journal	January 2012
Microsclerotial Enumeration, Size, and Survival of Calonectria pseudonaviculata Yang, Xiao; Hong, Chuanxue Plant Disease, Vol. 102, Issue 5 https://doi.org/10.1094/PDIS-08-17-1249-RE	journal	May 2018
Biological control of boxwood blight by Pseudomonas protegens recovered from recycling irrigation systems Yang, Xiao; Hong, Chuanxue Biological Control, Vol. 124 https://doi.org/10.1016/j.biocontrol.2018.01.014	journal	September 2018
Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection Ye, Xiaozhen; Liu, Hongyi; Jin, Yajie PLOS ONE, Vol. 12, Issue 1 https://doi.org/10.1371/journal.pone.0169598	journal	January 2017
Report of the Nomenclature Committee for Fungi: 20 May, TomW. Taxon, Vol. 66, Issue 2 https://doi.org/10.12705/662.15	journal	May 2017
Effectors as Tools in Disease Resistance Breeding Against Biotrophic, Hemibiotrophic, and Necrotrophic Plant Pathogens Vleeshouwers, Vivianne G. A. A.; Oliver, Richard P. Molecular Plant-Microbe Interactions, Vol. 2015, Issue 1 https://doi.org/10.1094/MPMI-10-13-0313-TA.testissue	journal	January 2015
Effectors as Tools in Disease Resistance Breeding Against Biotrophic, Hemibiotrophic, and Necrotrophic Plant Pathogens Vleeshouwers, Vivianne G. A. A.; Oliver, Richard P. Molecular Plant-Microbe Interactions https://doi.org/10.1094/mpmi-10-13-0313-ta.test	journal	October 2014
Effectors as Tools in Disease Resistance Breeding Against Biotrophic, Hemibiotrophic, and Necrotrophic Plant Pathogens Vleeshouwers, Vivianne G. A. A.; Oliver, Richard P. Molecular Plant-Microbe Interactions, Vol. 2015, Issue 1 https://doi.org/10.1094/mpmi-10-13-0313-cr.testissue	journal	January 2015
Population Genetics of Fungal and Oomycete Effectors Involved in Gene-for-Gene Interactions Stukenbrock, Eva H.; McDonald, Bruce A. Molecular Plant-Microbe Interactions®, Vol. 22, Issue 4 https://doi.org/10.1094/mpmi-22-4-0371	journal	April 2009
Leaf Blight of Buxus sempervirens in Northern Forests of Iran Caused by Calonectria pseudonaviculata Mirabolfathy, M.; Ahangaran, Y.; Lombard, L. Plant Disease, Vol. 97, Issue 8 https://doi.org/10.1094/pdis-03-13-0237-pdn	journal	August 2013
First Report of Box Blight Caused by Cylindrocladium pseudonaviculatum ( C. buxicola ) in British Columbia, Canada Elmhirst, J. F.; Auxier, B. E.; Wegener, L. A. Plant Disease, Vol. 97, Issue 4 https://doi.org/10.1094/pdis-10-12-0927-pdn	journal	April 2013
First Report of Boxwood Blight Caused by Calonectria pseudonaviculata in Delaware, Maryland, New Jersey, and New York Malapi-Wight, M.; Hébert, J. B.; Buckley, R. Plant Disease, Vol. 98, Issue 5 https://doi.org/10.1094/pdis-10-13-1102-pdn	journal	May 2014
First Report of Blight on Buxus spp. Caused by Cylindrocladium buxicola in Belgium Crepel, C.; Inghelbrecht, S. Plant Disease, Vol. 87, Issue 12 https://doi.org/10.1094/pdis.2003.87.12.1539a	journal	December 2003
Survival of Microsclerotia of Calonectria pseudonaviculata and C. henricotiae Exposed to Sanitizers Shishkoff, Nina Plant Health Progress, Vol. 17, Issue 1 https://doi.org/10.1094/php-rs-15-0038	journal	January 2016
Cylindrocladium buxicola causes common box blight in Croatia Cech, T.; Diminic, D.; Heungens, K. Plant Pathology, Vol. 59, Issue 6 https://doi.org/10.1111/j.1365-3059.2010.02361.x	journal	November 2010
Susceptibility of Boxwood Accessions from the National Boxwood Collection to Boxwood Blight and Potential for Differences between Calonectria pseudonaviculata and C. henricotiae LaMondia, James A.; Shishkoff, Nina HortScience, Vol. 52, Issue 6 https://doi.org/10.21273/hortsci11756-17	journal	June 2017

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