Binding partner- and force-promoted changes in αE-catenin conformation probed by native cysteine labeling

Terekhova, Ksenia; Pokutta, Sabine; Kee, Yee S.; Li, Jing; Tajkhorshid, Emad; Fuller, Gerald; Dunn, Alexander R.; Weis, William I.

doi:10.1038/s41598-019-51816-3

Title: Binding partner- and force-promoted changes in αE-catenin conformation probed by native cysteine labeling

Journal Article · Fri Oct 25 00:00:00 EDT 2019 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-019-51816-3· OSTI ID:1617175

Terekhova, Ksenia ^[1]; Pokutta, Sabine ^[1]; Kee, Yee S. ^[1]; Li, Jing ^[2]; Tajkhorshid, Emad ^[3]; Fuller, Gerald ^[1]; Dunn, Alexander R. ^[1]; Weis, William I. ^[1]

Stanford Univ., CA (United States)
Univ. of Chicago, IL (United States); Univ. of Illinois at Urbana-Champaign, IL (United States)
Univ. of Illinois at Urbana-Champaign, IL (United States)

Adherens Junctions (AJs) are cell-cell adhesion complexes that sense and propagate mechanical forces by coupling cadherins to the actin cytoskeleton via β-catenin and the F-actin binding protein αE-catenin. When subjected to mechanical force, the cadherin•catenin complex can tightly link to F-actin through αE-catenin, and also recruits the F-actin-binding protein vinculin. In this study, labeling of native cysteines combined with mass spectrometry revealed conformational changes in αE-catenin upon binding to the E-cadherin•β-catenin complex, vinculin and F-actin. A method to apply physiologically meaningful forces in solution revealed force-induced conformational changes in αE-catenin when bound to F-actin. Comparisons of wild-type αE-catenin and a mutant with enhanced vinculin affinity using cysteine labeling and isothermal titration calorimetry provide evidence for allosteric coupling of the N-terminal β-catenin-binding and the middle (M) vinculin-binding domain of αE-catenin. Cysteine labeling also revealed possible crosstalk between the actin-binding domain and the rest of the protein. The data provide insight into how binding partners and mechanical stress can regulate the conformation of full-length αE-catenin, and identify the M domain as a key transmitter of conformational changes.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1617175

Journal Information:: Scientific Reports, Vol. 9, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 10 works

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