Genome-Wide Analysis of Sorghum GT47 Family Reveals Functional Divergences of MUR3-Like Genes

Xu, Hua; Ding, Anming; Chen, Sihui; Marowa, Prince; Wang, Dian; Chen, Min; Hu, Ruibo; Kong, Yingzhen; O’Neill, Malcolm; Chai, Guohua; Zhou, Gongke

doi:10.3389/fpls.2018.01773

Title: Genome-Wide Analysis of Sorghum GT47 Family Reveals Functional Divergences of MUR3-Like Genes

Journal Article · Fri Dec 14 00:00:00 EST 2018 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2018.01773· OSTI ID:1611057

Xu, Hua ^[1]; Ding, Anming ^[2]; Chen, Sihui ^[1]; Marowa, Prince ^[2]; Wang, Dian ^[1]; Chen, Min ^[3]; Hu, Ruibo ^[1]; Kong, Yingzhen ^[2]; O’Neill, Malcolm ^[4]; Chai, Guohua ^[1]; Zhou, Gongke ^[1]

Chinese Academy of Sciences (CAS), Qingdao (China). Key Lab. of Biofuels, Qingdao Inst. of Bioenergy and Bioprocess Technology
Tobacco Research Inst. of Chinese Academy of Agricultural Sciences, Qingdao (China). Key Lab. of Tobacco Gene Resources
Shandong Normal Univ., Jinan (China). Shandong Provincial Key Lab. of Plant Stress Research
Univ. of Georgia, Athens, GA (United States). Complex Carbohydrate Research Center

Sorghum (Sorghum bicolor) is an important bioenergy crop. Its biomass mainly consists of the cellulosic and non-cellulosic polysaccharides, both which can be converted to biofuels. The biosynthesis of non-cellulosic polysaccharides involves several glycosyltransferases (GT) families including GT47. However, there was no systemic study on GT47 family in sorghum to date. Here, we identified 39 sorghum GT47 family members and showed the functional divergences of MURUS3 (MUR3) homologs. Sorghum GT47 proteins were phylogenetically clustered into four distinct subfamilies. Within each subfamily, gene structure was relatively conserved between the members. Ten gene pairs were identified from the 39 GT47 genes, of which two pairs might be originated from tandem duplication. 25.6% (10/39) of sorghum GT47 genes were homologous to Arabidopsis MUR3, a xyloglucan biosynthesis gene in primary cell walls. SbGT47_2, SbGT47_7, and SbGT47_8, three most homologous genes of MUR3, exhibited different tissue expression patterns and were selected for complementation into Arabidopsis mur3-3. Physiological and cell wall analyses showed that SbGT47_2 and SbGT47_7 may be two functional xyloglucan galactosyltransferases in sorghum. Further studies found that MUR3-like genes are widely present in the seed plants but not in the chlorophytic alga Chlamydomonas reinhardtii. Our results provide novel information for evolutionary analysis and functional dissection of sorghum GT47 family members.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Key Technology Support Program of China; National Natural Science Foundation of China (NSFC); Key Research and Development Program of Shandong Province

Grant/Contract Number:: SC0008472; FG02-12ER16324; 2015BAD15B03-05; 31670606; 51509237; 31470291; 31600237; 2017CXGC0309

OSTI ID:: 1611057

Journal Information:: Frontiers in Plant Science, Vol. 9; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 8 works

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Title: Genome-Wide Analysis of Sorghum GT47 Family Reveals Functional Divergences of MUR3-Like Genes

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