Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Durall, Claudia; Lindberg, Pia; Yu, Jianping; Lindbald, Peter

doi:10.1186/s13068-020-1653-y

Title: Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

Journal Article · Tue Jan 28 00:00:00 EST 2020 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-020-1653-y· OSTI ID:1598123

Durall, Claudia ^[1]; Lindberg, Pia ^[1]; Yu, Jianping ^[2]; Lindbald, Peter ^[1]

Uppsala Univ. (Sweden)
National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Cyanobacteria can be metabolically engineered to convert CO₂ to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules. The efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with increased phosphoenolpyruvate carboxylase (PEPc) levels. The resulting engineered strain (CD-P) showed significantly increased ethylene production (10.5 ± 3.1 ug mL^-1 OD^-1 day^-1) compared to the control strain (6.4 ± 1.4 ug mL^-1 OD^-1 day^-1). Interestingly, extra copies of the native pepc or the heterologous expression of PEPc from the cyanobacterium Synechococcus PCC 7002 (Synechococcus) in the CD-P, increased ethylene production (19.2 ± 1.3 and 18.3 ± 3.3 ug mL^-1 OD^-1 day^-1, respectively) when the cells were treated with the acetyl-CoA carboxylase inhibitor, cycloxydim. A heterologous expression of phosphoenolpyruvate synthase (PPSA) from Synechococcus in the CD-P also increased ethylene production (16.77 ± 4.48 ug mL^-1 OD^-1 day^-1) showing differences in the regulation of the native and the PPSA from Synechococcus in Synechocystis. This work demonstrates that genetic rewiring of cyanobacterial central carbon metabolism can enhance carbon supply to the TCA cycle and thereby further increase ethylene production.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office (BETO)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1598123

Report Number(s):: NREL/JA-2700-74702

Journal Information:: Biotechnology for Biofuels, Vol. 13, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 26 works

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09 BIOMASS FUELS
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Additional file 1 of Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.12010683.v1 The current record is supplemented by this text	text	January 2020
Additional file 1 of Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.12010683 The current record is supplemented by this text	text	January 2020
Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.c.4834587.v1 The current record is supplemented by this collection	collection	January 2020
Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.c.4834587.v2 The current record is supplemented by this collection	collection	January 2020
Additional file 1 of Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.11759928.v1 The current record is supplemented by this text	text	January 2020
Additional file 1 of Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.11759928 The current record is supplemented by this text	text	January 2020
Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping https://doi.org/10.6084/m9.figshare.c.4834587 The current record is supplemented by this collection	collection	January 2020

Title: Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803

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