Assembly of Methyl Coenzyme M Reductase in the Methanogenic Archaeon Methanococcus maripaludis

Lyu, Zhe; Chou, Chau-Wen; Shi, Hao; Wang, Liangliang; Ghebreab, Robel; Phillips, Dennis; Yan, Yajun; Duin, Evert C.; Whitman, William B.

doi:10.1128/JB.00746-17

Title: Assembly of Methyl Coenzyme M Reductase in the Methanogenic Archaeon Methanococcus maripaludis

Journal Article · Tue Jan 16 00:00:00 EST 2018 · Journal of Bacteriology

DOI:https://doi.org/10.1128/JB.00746-17· OSTI ID:1595760

^[1]; Chou, Chau-Wen ^[1]; Shi, Hao ^[1]; Wang, Liangliang ^[1]; Ghebreab, Robel ^[2]; Phillips, Dennis ^[1]; Yan, Yajun ^[1]; Duin, Evert C. ^[2]; Whitman, William B. ^[1]

Univ. of Georgia, Athens, GA (United States)
Auburn Univ., AL (United States)

Methyl coenzyme M reductase (MCR) is a complex enzyme that catalyzes the final step in biological methanogenesis. To better understand its assembly, the recombinant MCR from the thermophile Methanothermococcus okinawensis (rMCRok) was expressed in the mesophile Methanococcus maripaludis. The rMCRok was posttranslationally modified correctly and contained McrD and the unique nickel tetrapyrrole coenzyme F₄₃₀. Subunits of the native M. maripaludis (MCRmar) were largely absent, suggesting that the recombinant enzyme was formed by an assembly of cotranscribed subunits. Strong support for this hypothesis was obtained by expressing a chimeric operon comprising the His-tagged mcrA from M. maripaludis and the mcrBDCG from M. okinawensis in M. maripaludis. The His-tagged purified rMCR then contained the M. maripaludis McrA and the M. okinawensis McrBDG. The present study prompted us to form a working model for MCR assembly, which can be further tested by the heterologous expression system established here.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); Natural Science Foundation of Jiangsu Province

Grant/Contract Number:: SC0018028; BK20171266

OSTI ID:: 1595760

Journal Information:: Journal of Bacteriology, Vol. 200, Issue 7; ISSN 0021-9193

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 22 works

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03 NATURAL GAS
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59 BASIC BIOLOGICAL SCIENCES
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coenzyme F430
methyl coenzyme M reductase
posttranslational modification
Methanococcus
Methanothermococcus

Title: Assembly of Methyl Coenzyme M Reductase in the Methanogenic Archaeon Methanococcus maripaludis

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