Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species

Singer, Esther; Bonnette, Jason; Woyke, Tanja; Juenger, Thomas E.

doi:10.3389/fmicb.2019.02181

Title: Conservation of Endophyte Bacterial Community Structure Across Two Panicum Grass Species

Journal Article · Fri Sep 27 00:00:00 EDT 2019 · Frontiers in Microbiology

DOI:https://doi.org/10.3389/fmicb.2019.02181· OSTI ID:1580863

Singer, Esther ^[1]; Bonnette, Jason ^[2]; Woyke, Tanja ^[1]; Juenger, Thomas E. ^[2]

USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Univ. of Texas, Austin, TX (United States)

Panicum represents a large genus of many North American prairie grass species. These include switchgrass (Panicum virgatum), a biofuel crop candidate with wide geographic range, as well as Panicum hallii, a close relative to switchgrass, which serves as a model system for the study of Panicum genetics due to its diploid genome and short growth cycles. For the advancement of switchgrass as a biofuel crop, it is essential to understand host microbiome interactions, which can be impacted by plant genetics and environmental factors inducing ecotype-specific phenotypic traits. We here compared rhizosphere and root endosphere bacterial communities of upland and lowland P. virgatum and P. hallii genotypes planted at two sites in Texas. Our analysis shows that sampling site predominantly contributed to bacterial community variance in the rhizosphere, however, impacted root endosphere bacterial communities much less. Instead we observed a relatively large core endophytic microbiome dominated by ubiquitously root-colonizing bacterial genera Streptomyces, Pseudomonas, and Bradyrhizobium. Endosphere communities displayed comparable diversity and conserved community structures across genotypes of both Panicum species. Functional insights into interactions between P. hallii and its root endophyte microbiome could hence inform testable hypotheses that are relevant for the improvement of switchgrass as a biofuel crop.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Univ. of Texas, Austin, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division

Grant/Contract Number:: AC02-05CH11231; SC0014156

OSTI ID:: 1580863

Alternate ID(s):: OSTI ID: 1594755

Journal Information:: Frontiers in Microbiology, Vol. 10, Issue SEP; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 18 works

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