Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components

Patent · Tue Jul 30 00:00:00 EDT 2019

OSTI ID:1568650

Chan, James W.; Huser, Thomas R.; Lane, Stephen M.; Rutledge, John C.; Taylor, Douglas S.; Zwerdling, Theodore; Li, Ronald

Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: PHY-012099; W-7405-ENG-48
• Assignee: Lawrence Livermore National Security, LLC (Livermore, CA)
• Patent Number(s): 10,365,220
• Application Number: 11/916,273
• OSTI ID: 1568650
• Resource Relation: Patent File Date: 05/31/2006
• Country of Publication: United States
• Language: English