DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function

Serrano, Christopher M.; Freeman, Ronit; Godbe, Jacqueline; Lewis, Jacob A.; Stupp, Samuel I.

doi:10.1021/acsabm.9b00310

Title: DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function

Journal Article · Tue Jun 18 00:00:00 EDT 2019 · ACS Applied Bio Materials

DOI:https://doi.org/10.1021/acsabm.9b00310· OSTI ID:1557289

Serrano, Christopher M. ^[1]; Freeman, Ronit ^[2]; Godbe, Jacqueline ^[1]; Lewis, Jacob A. ^[1];

^[1]

Northwestern Univ., Evanston, IL (United States)
Northwestern Univ., Evanston, IL (United States); Univ. of North Carolina, Chapel Hill, NC (United States)

The single-stranded DNA oligonucleotides known as aptamers have the capacity to bind proteins and other molecules and offer great therapeutic potential. Further work is required to optimize their function and to diminish their susceptibility to nuclease degradation. We report here on the synthesis and supramolecular self-assembly of DNA-peptide amphiphiles that form high aspect ratio nanofibers and display aptamers for the platelet-derived growth factor. The nanofibers were found to bind the growth factor with an affinity that was 5-fold greater than the free aptamer. Additionally, we also observed that the aptamer displayed by the supramolecular nanostructures was eight times more nuclease-resistant than the free aptamer. In order to highlight the therapeutic potential of these supramolecular systems, we demonstrated the improved inhibition of proliferation when the growth factor was bound to aptamers displayed by the nanofibers.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-06CH11357; 2R01DE015920–11; ALTF 233–2012; ECCS-1542205; P30 CA060553

OSTI ID:: 1557289

Journal Information:: ACS Applied Bio Materials, Vol. 2, Issue 7; ISSN 2576-6422

Publisher:: ACS PublicationsCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
biomaterials
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Title: DNA-Peptide Amphiphile Nanofibers Enhance Aptamer Function

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