4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase

Barros, Jaime; Escamilla-Trevino, Luis; Song, Luhua; Rao, Xiaolan; Serrani-Yarce, Juan Carlos; Palacios, Maite Docampo; Engle, Nancy; Choudhury, Feroza K.; Tschaplinski, Timothy J.; Venables, Barney J.; Mittler, Ron; Dixon, Richard A.

doi:10.1038/s41467-019-10082-7

Title: 4-Coumarate 3-hydroxylase in the lignin biosynthesis pathway is a cytosolic ascorbate peroxidase

Journal Article · Tue Apr 30 00:00:00 EDT 2019 · Nature Communications

DOI:https://doi.org/10.1038/s41467-019-10082-7· OSTI ID:1545188

^[1]; Escamilla-Trevino, Luis ^[1]; Song, Luhua ^[2]; Rao, Xiaolan ^[1]; Serrani-Yarce, Juan Carlos ^[2];

^[2];

^[3]; Choudhury, Feroza K. ^[2];

^[3]; Venables, Barney J. ^[2]; Mittler, Ron ^[2];

^[1]

Univ. of North Texas, Denton, TX (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of North Texas, Denton, TX (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Lignin biosynthesis is evolutionarily conserved among higher plants and features a critical 3-hydroxylation reaction involving phenolic esters. Yet, increasing evidence questions the involvement of a single pathway to lignin formation in vascular plants. In this work we describe an enzyme catalyzing the direct 3-hydroxylation of 4-coumarate to caffeate in lignin biosynthesis as a bifunctional peroxidase that oxidizes both ascorbate and 4-coumarate at comparable rates. A combination of biochemical and genetic evidence in the model plants Brachypodium distachyon and Arabidopsis thaliana supports a role for this coumarate 3-hydroxylase (C3H) in the early steps of lignin biosynthesis. The subsequent efficient O-methylation of caffeate to ferulate in grasses is substantiated by in vivo biochemical assays. Our results identify C3H as the only non-membrane bound hydroxylase in the lignin pathway and revise the currently accepted models of lignin biosynthesis, suggesting new gene targets to improve forage and bioenergy crops.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1545188

Journal Information:: Nature Communications, Vol. 10, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 118 works

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Additional file 2 of Genome-wide characterization of 2-oxoglutarate and Fe(II)-dependent dioxygenase family genes in tomato during growth cycle and their roles in metabolism Wei, Shuo; Zhang, Wen; Fu, Rao https://doi.org/10.6084/m9.figshare.14059431.v1 The current record is supplemented by this dataset	dataset	January 2021
Additional file 3 of Genome-wide characterization of 2-oxoglutarate and Fe(II)-dependent dioxygenase family genes in tomato during growth cycle and their roles in metabolism Wei, Shuo; Zhang, Wen; Fu, Rao https://doi.org/10.6084/m9.figshare.14059434.v1 The current record is supplemented by this dataset	dataset	January 2021
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