Amplification and cloning of single DNA molecules using rolling circle amplification

Hutchison, III, Clyde A.; Smith, Hamilton O.

Title: Amplification and cloning of single DNA molecules using rolling circle amplification

Patent · Tue Jul 30 00:00:00 EDT 2013

OSTI ID:1531861

Hutchison, III, Clyde A.; Smith, Hamilton O.

The present invention relates, e.g., to a method for amplifying a small number of copies (e.g. a single copy) of a single-stranded circular DNA molecule (e.g. having a size of about 5-6 kb) by an isothermal rolling circle mechanism, using random or partially random primers and a F29-type DNA polymerase. The method, which can also be used for amplifying DNAs by non-rolling types of multiple displacement amplification, comprises incubating the reaction components in a small volume, e.g. about 10 μl or less, such as about 0.6 μl or less. The degree of amplification can be about 109 fold, or higher. A method for cell-free cloning of DNA, using the rolling circle amplification method of the invention, is described.

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Research Organization:: Synthetic Genomics, Inc., San Diego, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-02ER63453

Assignee:: Synthetic Genomics, Inc. (San Diego, CA)

Patent Number(s):: 8,497,069

Application Number:: 11/919,515

OSTI ID:: 1531861

Resource Relation:: Patent File Date: 2006-05-01

Country of Publication:: United States

Language:: English

References (16)

TempliPhi, ⌽29 DNA Polymerase Based Rolling Circle Amplification of Templates for DNA Sequencing Nelson, John R.; Cai, Yuyang Christine; Giesler, Theresa L. BioTechniques, Vol. 32, Issue 6S https://doi.org/10.2144/jun0208	journal	June 2002
Method for amplification of nucleic acids in solid media Chetverin, Alexander B.; Chetverina, Helena Vladimirovna https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5616478 US Patent Document 5,616,478	patent	April 1997
Methods of amplifying and sequencing nucleic acids Leamon, John H.; Lohman, Kenton L.; Rothberg, Jonathan M. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050130173 US Patent Application 10/767779; 20050130173	patent-application	June 2005
Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics Detter, John C.; Jett, Jamie M.; Lucas, Susan M. Genomics, Vol. 80, Issue 6 https://doi.org/10.1006/geno.2002.7020	journal	December 2002
Convergent DNA synthesis: a non-enzymatic dimerization approach to circular oligodeoxynucleotides Rubin, Ethel; Rumney, Squire; Wang, Shaohui Nucleic Acids Research, Vol. 23, Issue 17 https://doi.org/10.1093/nar/23.17.3547	journal	January 1995
Rapid thermocycling for sample analysis Landers, James P.; Huhmer, Andreas; Oda, Robert P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20010029036 US Patent Application 09/759892; 20010029036	patent-application	October 2001
Method of amplifying whole genomes without subjecting the genome to denaturing conditions Dean, Frank B.; Lasken, Roger https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6617137 US Patent Document 6,617,137	patent	September 2003
Rolling Circle DNA Synthesis: Small Circular Oligonucleotides as Efficient Templates for DNA Polymerases Liu, Dongyu; Daubendiek, Sarah L.; Zillman, Martin A. Journal of the American Chemical Society, Vol. 118, Issue 7 https://doi.org/10.1021/ja952786k	journal	January 1996
Cell-free cloning using φ29 DNA polymerase Hutchison, C. A.; Smith, H. O.; Pfannkoch, C. Proceedings of the National Academy of Sciences, Vol. 102, Issue 48, p. 17332-17336 https://doi.org/10.1073/pnas.0508809102	journal	November 2005
A “Double Adaptor” Method for Improved Shotgun Library Construction Andersson, Björn; Wentland, Meredith A.; Ricafrente, Jennifer Y. Analytical Biochemistry, Vol. 236, Issue 1 https://doi.org/10.1006/abio.1996.0138	journal	April 1996
Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification Dean, F. B. Genome Research, Vol. 11, Issue 6 https://doi.org/10.1101/gr.180501	journal	June 2001
A Sequence-Independent Strategy for Detection and Cloning of Circular DNA Virus Genomes by Using Multiply Primed Rolling-Circle Amplification Rector, Annabel; Tachezy, Ruth; Van Ranst, Marc Journal of Virology, Vol. 78, Issue 10 https://doi.org/10.1128/JVI.78.10.4993-4998.2004	journal	May 2004
Rolling replication of short DNA circles. Fire, A.; Xu, S. Q. Proceedings of the National Academy of Sciences, Vol. 92, Issue 10 https://doi.org/10.1073/pnas.92.10.4641	journal	May 1995
Human parainfluenza virus-1 assay Henrickson, Kelly; Fan, Jiang https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5744299 US Patent Document 5,744,299	patent	April 1998
In situ localized amplification and contact replication of many individual DNA molecules Mitra, R. Nucleic Acids Research, Vol. 27, Issue 24 https://doi.org/10.1093/nar/27.24.e34	journal	December 1999
Single-molecule PCR using water-in-oil emulsion Nakano, Michihiko; Komatsu, Jun; Matsuura, Shun-ichi Journal of Biotechnology, Vol. 102, Issue 2, p. 117-124 https://doi.org/10.1016/S0168-1656(03)00023-3	journal	April 2003

Cited By (1)

Individually synthesized primers to be used in whole genome amplification Nazarenko, Irina; O'Neil, Dominic; Thai, Ha https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9593366 US Patent Document 9,593,366	patent	March 2017

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