skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microbial drivers of global change at the aggregate scale: linking genomic function to carbon metabolism and warming

Technical Report ·
DOI:https://doi.org/10.2172/1524429· OSTI ID:1524429
ORCiD logo [1]
  1. Iowa State Univ., Ames, IA (United States); Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Understanding and accurately predicting the microbial cycling of carbon in soil environments has been challenged by our ability to associate microbial community dynamics into ecosystem-scale biogeochemical models. Soil fractionation techniques provide an opportunity to examine intact microbial communities in a context that is relevant to both microbial community metabolism and ecosystem processes. We developed approaches that targeted metabolically active microorganisms and functions that drive carbon cycling in soils from bioenergy cropping systems. We used metatranscriptomics, genome sequencing, cell sorting, and cell isolation methods to access the key organisms involved in soil carbon cycling (e.g. cellulose decomposition) in soil aggregate fractions.

Research Organization:
Iowa State Univ., Ames, IA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER). Biological Systems Science Division
DOE Contract Number:
SC0010775
OSTI ID:
1524429
Report Number(s):
Final-Report-DOE-IowaState-0010775
Country of Publication:
United States
Language:
English

References (10)

Strategies to improve reference databases for soil microbiomes journal December 2016
Identification of the Core Set of Carbon-Associated Genes in a Bioenergy Grassland Soil journal November 2016
The dynamic life of arbuscular mycorrhizal fungal symbionts journal January 2018
Demonstrating microbial co-occurrence pattern analyses within and between ecosystems journal July 2014
Greatest soil microbial diversity found in micro-habitats journal March 2018
Spatial Structuring of Cellulase Gene Abundance and Activity in Soil journal October 2018
Belowground response of prairie restoration and resiliency to drought journal November 2018
Spatio-temporal microbial community dynamics within soil aggregates journal May 2019
Soil aggregate isolation method affects measures of intra-aggregate extracellular enzyme activity journal February 2014
Coupled Carbon and Nitrogen Inputs Increase Microbial Biomass and Activity in Prairie Bioenergy Systems journal January 2015