Molecular architecture of fungal cell walls revealed by solid-state NMR

Kang, Xue; Kirui, Alex; Muszyński, Artur; Widanage, Malitha C.  Dickwella; Chen, Adrian; Azadi, Parastoo; Wang, Ping; Mentink-Vigier, Frederic; Wang, Tuo

doi:10.1038/s41467-018-05199-0

Title: Molecular architecture of fungal cell walls revealed by solid-state NMR

Journal Article · Mon Jul 16 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-05199-0· OSTI ID:1511439

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Louisiana State Univ., Baton Rouge, LA (United States)
Univ. of Georgia, Athens, GA (United States)
Louisiana State Univ. Health Sciences Center, New Orleans, LA (United States)
Florida State Univ., Tallahassee, FL (United States). National High Magnetic Field Lab. (MagLab)

The high mortality of invasive fungal infections, and the limited number and inefficacy of antifungals necessitate the development of new agents with novel mechanisms and targets. The fungal cell wall is a promising target as it contains polysaccharides absent in humans, however, its molecular structure remains elusive. Here we report the architecture of the cell walls in the pathogenic fungus Aspergillus fumigatus. Solid-state NMR spectroscopy, assisted by dynamic nuclear polarization and glycosyl linkage analysis, reveals that chitin and α-1,3-glucan build a hydrophobic scaffold that is surrounded by a hydrated matrix of diversely linked β-glucans and capped by a dynamic layer of glycoproteins and α-1,3-glucan. The two-domain distribution of α-1,3-glucans signifies the dual functions of this molecule: contributing to cell wall rigidity and fungal virulence. This study provides a high-resolution model of fungal cell walls and serves as the basis for assessing drug response to promote the development of wall-targeted antifungals.

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Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences, and Biosciences Division

Grant/Contract Number:: SC0015662

OSTI ID:: 1511439

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 141 works

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Longitudinal Relaxation Optimization Enhances ¹ H‐Detected HSQC in Solid‐State NMR Spectroscopy on Challenging Biological Systems Han, Rong; Yang, Yufei; Wang, Shenlin Chemistry – A European Journal, Vol. 25, Issue 16 https://doi.org/10.1002/chem.201805327	journal	February 2019
Circulating (1→3)-β-D-glucan Is Associated With Immune Activation During Human Immunodeficiency Virus Infection Mehraj, Vikram; Ramendra, Rayoun; Isnard, Stéphane Clinical Infectious Diseases, Vol. 70, Issue 2 https://doi.org/10.1093/cid/ciz212	journal	March 2019
Molecular architecture of softwood revealed by solid-state NMR Terrett, Oliver M.; Lyczakowski, Jan J.; Yu, Li Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.59388	text	January 2019
Aspergillus fumigatus and Aspergillosis in 2019 Latgé, Jean-Paul; Chamilos, Georgios Clinical Microbiology Reviews, Vol. 33, Issue 1 https://doi.org/10.1128/cmr.00140-18	journal	November 2019
The puzzling construction of the conidial outer layer of Aspergillus fumigatus Valsecchi, Isabel; Dupres, Vincent; Michel, Jean‐Philippe Cellular Microbiology, Vol. 21, Issue 5 https://doi.org/10.1111/cmi.12994	journal	January 2019
Integration of electron microscopy and solid-state NMR analysis for new views and compositional parameters of Aspergillus fumigatus biofilms Reichhardt, Courtney; Joubert, Lydia-Marie; Clemons, Karl V. Medical Mycology, Vol. 57, Issue Supplement_2 https://doi.org/10.1093/mmy/myy140	journal	February 2019
FGB1 and WSC3 are in planta‐ induced β ‐glucan‐binding fungal lectins with different functions Wawra, Stephan; Fesel, Philipp; Widmer, Heidi New Phytologist, Vol. 222, Issue 3 https://doi.org/10.1111/nph.15711	journal	February 2019
The melanization road more traveled by: Precursor substrate effects on melanin synthesis in cell-free and fungal cell systems Chatterjee, Subhasish; Prados-Rosales, Rafael; Tan, Sindy Journal of Biological Chemistry, Vol. 293, Issue 52 https://doi.org/10.1074/jbc.ra118.005791	journal	November 2018
Molecular architecture of softwood revealed by solid-state NMR. Terrett, Oliver; Lyczakowski, Jan; Yu, Li Apollo - University of Cambridge Repository https://doi.org/10.17863/cam.44837	text	January 2019

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