Molecular response of poplar to single and combined ozone and drought

Journal Article · Thu Nov 15 00:00:00 EST 2018 · Science of the Total Environment

DOI:https://doi.org/10.1016/j.scitotenv.2018.11.195· OSTI ID:1503995

Zhang, Jin  ^[1]; Gao, Feng ^[2]; Jia, Huixia ^[3]; Hu, Jianjun  ^[3]; Feng, Zhaozhong ^[2]

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1. Chinese Academy of Sciences (CAS), Beijing (China); Chinese Academy of Forestry, Beijing (China); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
2. Chinese Academy of Sciences (CAS), Beijing (China)
3. Chinese Academy of Forestry, Beijing (China)

High concentration in ground-level ozone (O₃) and water deficit affect forest ecosystems service. Earlier we found intercellular CO₂ concentration and isoprene emission were affected by the combination of O₃ and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we examined the stomatal conductance (g_s) and transcriptome changes in an O₃-sensitive hybrid poplar exposed to two O₃ levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the g_s more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O₃. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O₃ and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O₃ and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to the combination of O₃ and drought.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC05-00OR22725
• OSTI ID: 1503995
• Journal Information: Science of the Total Environment, Vol. 655, Issue C; ISSN 0048-9697
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Climate change
Drought
Ground-level ozone
Poplar
Transcriptome
Helitrons
Protein-protein interaction (PPI) network