A Novel Poplar Biomass Germplasm Resource for Functional Genomics and Breeding
Interspecific hybridization is the most effective method for producing poplar clones for commercial applications, including biomass production. Previous research has shown that commercial F1 hybrids frequently exhibit dosage variation, i.e. variation in the number of pieces or entire chromosomes, and that these variants can have novel or more extreme phenotypes compared to their parents, that can be desirable for biomass production. Poplar is a convenient system for studying and exploiting gene dosage because germplasm can very easily be propagated vegetatively, via cuttings, for research and production, and advanced genome-enabled tools can be directly employed on the actual feedstock. In the previous funding cycle, we had produced and characterized a population of interspecific hybrids carrying insertions and deletions, which together, tile the entire genome with dosage variation. In this funding cycle, we completed the development of this unique resource for the research community and started using the germplasm to investigate the role of gene dosage in poplar hybrid performance, and to identify chromosomal regions and genes that contribute to poplar bioenergy traits. Our specific goals were to: i) maintain and finish the characterization of this resource ii) investigate the phenotypic effects of dosage change iii) exploit the indel germplasm for functional genomics.
- Research Organization:
- Univ. of California, Davis, CA (United States)
- Sponsoring Organization:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- Contributing Organization:
- USDA Forest Service
- DOE Contract Number:
- SC0007183
- OSTI ID:
- 1502959
- Report Number(s):
- FinalReport-DOE-UCDavis-SC0006999
- Country of Publication:
- United States
- Language:
- English
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