Bacteria engineered for conversion of ethylene to ethanol

Atsumi, Shota; Toney, Michael D.; Rodriguez, Gabriel M.; Tashiro, Yohei; Siegel, Justin B.; Carlin, D. Alexander; Koryakina, Irina; Desai, Shuchi H.

Title: Bacteria engineered for conversion of ethylene to ethanol

Patent · Tue Jan 22 00:00:00 EST 2019

OSTI ID:1498702

Atsumi, Shota; Toney, Michael D.; Rodriguez, Gabriel M.; Tashiro, Yohei; Siegel, Justin B.; Carlin, D. Alexander; Koryakina, Irina; Desai, Shuchi H.

The present disclosure provides recombinant bacteria with elevated production of ethanol and/or n-butanol from ethylene. Methods for the production of the recombinant bacteria, as well as for use thereof for production of ethanol and/or n-butanol are also provided.

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Research Organization:: Univ. of California, Oakland, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AR0000429

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 10,184,138

Application Number:: 15/317,656

OSTI ID:: 1498702

Resource Relation:: Patent File Date: 2019 Jan 22

Country of Publication:: United States

Language:: English

References (11)

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Oleate Hydratase Catalyzes the Hydration of a Nonactivated Carbon-Carbon Bond Bevers, L. E.; Pinkse, M. W. H.; Verhaert, P. D. E. M. Journal of Bacteriology, Vol. 191, Issue 15 https://doi.org/10.1128/JB.00306-09	journal	May 2009
Heterologous expression of alkene monooxygenase from Rhodococcus rhodochrous B-276: Heterologous expression of AMO from R.��rhodochrous B-276 Smith, Thomas J.; Lloyd, John S.; Gallagher, Stephen C. European Journal of Biochemistry, Vol. 260, Issue 2 https://doi.org/10.1046/j.1432-1327.1999.00179.x	journal	March 1999
Ethanol production using engineered mutant E. coli Ingram, Lonnie O.; Clark, David P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5028539 US Patent Document 5,028,539	patent	July 1991
DNA fragment comprising a gene encoding ethylene forming enzyme of bacteria and the use thereof Fukuda, Hideo; Ogawa, Takahira; Fujii, Takao https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/5536659 US Patent Document 5,536,659	patent	July 1996
Biocatalytic conversion of ethylene to ethylene oxide using an engineered toluene monooxygenase Carlin, D. A.; Bertolani, S. J.; Siegel, J. B. Chemical Communications, Vol. 51, Issue 12, p. 2283-2285 https://doi.org/10.1039/C4CC08802F	journal	January 2015
Biological conversion of gaseous alkenes to liquid chemicals Desai, Shuchi H.; Koryakina, Irina; Case, Anna E. Metabolic Engineering, Vol. 38 https://doi.org/10.1016/j.ymben.2016.07.002	journal	November 2016
2-Haloacrylate Hydratase, a New Class of Flavoenzyme That Catalyzes the Addition of Water to the Substrate for Dehalogenation Mowafy, A. M.; Kurihara, T.; Kurata, A. Applied and Environmental Microbiology, Vol. 76, Issue 18 https://doi.org/10.1128/AEM.00334-10	journal	July 2010
Genetically Modified Cyanobacteria for the Production of Ethanol Woods, R. Paul; Smith, Craig; Kramer, Dan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100068776 US Patent Document 12/368060; 20100068776	patent-application	March 2010
Driving Forces Enable High-Titer Anaerobic 1-Butanol Synthesis in Escherichia coli Shen, Claire R.; Lan, Ethan I.; Dekishima, Yasumasa Applied and Environmental Microbiology, Vol. 77, Issue 9, p. 2905-2915 https://doi.org/10.1128/AEM.03034-10	journal	March 2011
Novel Genes for the Fermentative Production of Hydroxytyrosol Achkar, Jihane; Ferrandez, Abel; Sonke, Theodorus https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100068775 US Patent Application 12/516415; 20100068775	patent-application	March 2010

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