Microfluidic platform for synthetic biology applications

Patent · Tue Nov 20 00:00:00 EST 2018

OSTI ID:1494577

Chang, Chieh; Bharadwa, Rajiv; Singh, Anup K.; Chandrasekaran, Aarthi; Hillson, Nathan J.

This invention provides methods and compositions for assembling biological constructs (e.g., plasmids, transformed cells, etc.). In certain embodiments the methods involve encapsulating separate components of the biological construct each in a fluid droplet confined in a fluid channel; optionally mixing droplets from different fluid channels to form a sequenced order of droplets carrying different components of the biological construct in a channel or chamber; and optionally combining two or more droplets each containing different components of the biological construct to permit the components to react with each other in one or more reactions contributing to the assembly of the biological construct.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC02-05CH11231
• Assignee: The Regents of the University of California (Oakland, CA); Sandia Corporation (Livermore, CA)
• Patent Number(s): 10,131,903
• Application Number: 13/437,727
• OSTI ID: 1494577
• Resource Relation: Patent File Date: 2012 Apr 02
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES