Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

McVey, Patrick A.; Alexander, Liza E.; Fu, Xinyu; Xie, Bo; Galayda, Katherine -Jo; Nikolau, Basil J.; Houk, Robert S.

doi:10.3389/fpls.2018.01348

Title: Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

Journal Article · Wed Sep 19 00:00:00 EDT 2018 · Frontiers in Plant Science

DOI:https://doi.org/10.3389/fpls.2018.01348· OSTI ID:1478236

McVey, Patrick A. ^[1]; Alexander, Liza E. ^[1]; Fu, Xinyu ^[1]; Xie, Bo ^[2]; Galayda, Katherine -Jo ^[3]; Nikolau, Basil J. ^[1]; Houk, Robert S. ^[1]

Ames Lab. and Iowa State Univ., Ames, IA (United States)
Ames Lab., Ames, IA (United States)
Ames Lab. and Iowa State Univ., Ames, IA (United States); Kemin Industries Inc., Des Moines, IA (United States)

The visualization of foliage color in plants provides immediate insight into some of the compounds that exist in the leaf. However, many non-colored compounds are also present; their cellular distributions are not readily identifiable optically. In this study we evaluate the applicability of mass spectrometry imaging (MSI) via electrospray laser desorption ionization (ELDI) to reveal the spatial distribution of metabolites. ELDI-MSI is a matrix free, atmospheric pressure ionization method that utilizes a UV laser coupled with supplemental ionization by electrospray. We specifically applied ELDI-MSI to determine the spatial distribution of metabolites in Coleus Henna half leaves that were grown with half-sections either fully illuminated or shaded. We monitored dynamic changes in the spatial distribution of metabolites in response to the change of illumination every 7 days for a 28 day period. A novel source-sink relationship was observed between the 2 halves of the experimental leaf. Furthermore, Coleus Henna leaves present visually recognizable sectors associated with the differential accumulation of flavonoids. Thus, we correlated the effect of differential illumination and presence or absence of flavonoids with metabolic changes revealed by the accumulation of carbohydrates, amino acids, and organic acids. Here, the results show the potential of ELDI-MSI to provide spatial information for a variety of plant metabolites with little sample preparation.

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Research Organization:: Ames Laboratory (AMES), Ames, IA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-07CH11358

OSTI ID:: 1478236

Report Number(s):: IS-J-9768

Journal Information:: Frontiers in Plant Science, Vol. 9; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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59 BASIC BIOLOGICAL SCIENCES
matrix-free imaging
mass spectrometry imaging
electrospray laser desorption ionization
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Coleus (Solenostemon scuttellarioides)
anthocyanin
flavonoid
source-to-sink

Title: Light-Dependent Changes in the Spatial Localization of Metabolites in Solenostemon scutellarioides (Coleus Henna) Visualized by Matrix-Free Atmospheric Pressure Electrospray Laser Desorption Ionization Mass Spectrometry Imaging

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