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Title: The Thermodynamic Links between Substrate, Enzyme, and Microbial Dynamics in Michaelis-Menten-Monod Kinetics

Journal Article · · International Journal of Chemical Kinetics
DOI:https://doi.org/10.1002/kin.21163· OSTI ID:1477285
 [1];  [1];  [2]
  1. Univ. of Sydney, NSW (Australia). School of Civil Engineering
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Earth Sciences Division
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Accurate prediction of the temperature response of the velocity v of a biochemical reaction has wide applications in cell biology, reaction design, and biomass yield enhancement. Here in this study, we introduce a simple but comprehensive mechanistic approach that uses thermodynamics and biochemical kinetics to describe and link the reaction rate and Michaelis–Menten constants (kT and KT) with the biomass yield and mortality rate (YT and δT) as explicit functions of T. The temperature control is exerted by catabolic enthalpy at low temperatures and catabolic entropy at high temperatures, whereas changes in cell and enzyme–substrate heat capacity shift the anabolic electron use efficiency eA and the maximum reaction velocity vmax. We show that cells have optimal growth when the catabolic (differential) free energy of activation decreases the cell free energy harvest required to duplicate their internal structures as long as electrons for anabolism are available. Lastly, with the described approach, we accurately predicted observed glucose fermentation and ammonium nitrification dynamics across a wide temperature range with a minimal number of thermodynamics parameters, and we highlight how kinetic parameters are linked to each other using first principles.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1477285
Journal Information:
International Journal of Chemical Kinetics, Vol. 50, Issue 5; ISSN 0538-8066
Publisher:
WileyCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

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