A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion

Mallinson, Sam J. B.; Machovina, Melodie M.; Silveira, Rodrigo L.; Garcia-Borras, Marc; Gallup, Nathan; Johnson, Christopher W.; Allen, Mark D.; Skaf, Munir S.; Crowley, Michael F.; Neidle, Ellen L.; Houk, Kendall N.; Beckham, Gregg T.; DuBois, Jennifer L.; McGeehan, John E.

doi:10.1038/s41467-018-04878-2

Title: A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion

Journal Article · Wed Jun 27 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-04878-2· OSTI ID:1458818

Mallinson, Sam J. B. ^[1];

^[2]; Silveira, Rodrigo L. ^[3];

^[4]; Gallup, Nathan ^[5];

^[6]; Allen, Mark D. ^[1];

^[7];

^[6];

^[8]; Houk, Kendall N. ^[4];

^[6]; DuBois, Jennifer L. ^[9];

^[1]

Univ. of Portsmouth, Portsmouth (United Kingdom)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Montana State Univ., Bozeman, MT (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Campinas, Sao Paulo (Brazil)
Univ. of California at Los Angeles, Los Angeles, CA (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of California at Los Angeles, Los Angeles, CA (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Univ. of Campinas, Sao Paulo (Brazil)
Univ. of Georgia, Athens, GA (United States)
Montana State Univ., Bozeman, MT (United States)

Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Here, structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1458818

Report Number(s):: NREL/JA-5100-71206

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 89 works

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Influence of organic amendments used for benz[a]anthracene remediation in a farmland soil: pollutant distribution and bacterial changes Zhu, Qinghe; Wu, Yucheng; Zeng, Jun Journal of Soils and Sediments, Vol. 20, Issue 1 https://doi.org/10.1007/s11368-019-02368-6	journal	June 2019
Bacterial conversion of depolymerized Kraft lignin Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1397-8	journal	March 2019
Specificity and mechanism of carbohydrate demethylation by cytochrome P450 monooxygenases Robb, Craig S.; Reisky, Lukas; Bornscheuer, Uwe T. Biochemical Journal, Vol. 475, Issue 23 https://doi.org/10.1042/BCJ20180762	journal	December 2018
Modular Engineering of Biomass Degradation Pathways Chaves, Julie E.; Presley, Gerald N.; Michener, Joshua K. Processes, Vol. 7, Issue 4 https://doi.org/10.3390/pr7040230	journal	April 2019
Bacterial cytochrome P450-catalyzed regio- and stereoselective steroid hydroxylation enabled by directed evolution and rational design Zhang, Xiaodong; Peng, Yaqin; Zhao, Jing Bioresources and Bioprocessing, Vol. 7, Issue 1 https://doi.org/10.1186/s40643-019-0290-4	journal	January 2020
Passive membrane transport of lignin-related compounds Vermaas, Josh V.; Dixon, Richard A.; Chen, Fang Proceedings of the National Academy of Sciences, Vol. 116, Issue 46 https://doi.org/10.1073/pnas.1904643116	journal	October 2019
Mapping the diversity of microbial lignin catabolism: experiences from the eLignin database Brink, Daniel P.; Ravi, Krithika; Lidén, Gunnar Applied Microbiology and Biotechnology, Vol. 103, Issue 10 https://doi.org/10.1007/s00253-019-09692-4	journal	April 2019

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