Heat-stable, FE-dependent alcohol dehydrogenase for aldehyde detoxification

Elkins, James G.; Clarkson, Sonya

Title: Heat-stable, FE-dependent alcohol dehydrogenase for aldehyde detoxification

Patent · Tue Apr 24 00:00:00 EDT 2018

OSTI ID:1436517

Elkins, James G.; Clarkson, Sonya

The present invention relates to microorganisms and polypeptides for detoxifying aldehydes associated with industrial fermentations. In particular, a heat-stable, NADPH- and iron-dependent alcohol dehydrogenase was cloned from Thermoanaerobacter pseudethanolicus 39E and displayed activity against a number of aldehydes including inhibitory compounds that are produced during the dilute-acid pretreatment process of lignocellulosic biomass before fermentation to biofuels. Methods to use the microorganisms and polypeptides of the invention for improved conversion of bio mass to biofuel are provided as well as use of the enzyme in metabolic engineering strategies for producing longer-chain alcohols from sugars using thermophilic, fermentative microorganisms.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-00OR22725

Assignee:: UT-BATTELLE, LLC (Oak Ridge, TN)

Patent Number(s):: 9,951,359

Application Number:: 15/060,078

OSTI ID:: 1436517

Resource Relation:: Patent File Date: 2016 Mar 03

Country of Publication:: United States

Language:: English

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