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Title: Funnel for localizing biological cell placement and arrangement

Patent ·
OSTI ID:1425913

The present disclosure relates to a funnel apparatus for channeling cells onto a plurality of distinct, closely spaced regions of a seeding surface. The funnel apparatus has a body portion having an upper surface and a lower surface. The body portion forms a plurality of flow paths, at least one of which is shaped to have a decreasing cross-sectional area from the upper surface to the lower surface. The flow paths are formed at the lower surface to enable cells deposited into the flow paths at the upper surface of the funnel apparatus to be channeled into a plurality of distinct, closely spaced regions on the seeding surface positioned adjacent the lower surface.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC52-07NA27344
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
9,909,093
Application Number:
14/824,857
OSTI ID:
1425913
Resource Relation:
Patent File Date: 2015 Aug 12
Country of Publication:
United States
Language:
English

References (11)

Peelable and resealable devices for arraying materials patent-application April 2003
Cell interaction culture system and uses thereof patent-application October 2004
Microfluidic particle-analysis systems patent-application November 2004
Tracking Biological and Other Samples Using RFID Tags patent-application August 2011
Device for Sampling and Transporting Nano-Objects Contained in Aerosols for the Analysis Thereof patent-application September 2014
Nanofiber Mats, Method of Manufacturing the Nanofiber Mats, and Applications to Cell Culture and Nanofibrous Membrane for Guided Bone Regeneration patent-application September 2016
Cell-Seeding and -Culturing Device patent-application January 2017
Large-Scale Single-Cell Trapping And Imaging Using Microwell Arrays journal September 2005
3-D microwell culture of human embryonic stem cells journal December 2006
Patterning Mammalian Cells Using Elastomeric Membranes journal October 2000
Patterning proteins and cells using soft lithography journal December 1999

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