skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus

Journal Article · · Frontiers in Plant Science
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Georgia, Athens, GA (United States)
+ Show Author Affiliations

Cortical microtubules (MTs) are evolutionarily conserved cytoskeletal components with specialized roles in plants, including regulation of cell wall biogenesis. MT functions and dynamics are dictated by the composition of their monomeric subunits, α- (TUA) and β-tubulins (TUB), which in animals and protists are subject to both transcriptional regulation and post-translational modifications (PTM). While spatiotemporal regulation of tubulin gene expression has been reported in plants, whether and to what extent tubulin PTMs occur in these species remain poorly understood. We chose the woody perennial Populus for investigation of tubulin PTMs in this study, with a particular focus on developing xylem where high tubulin transcript levels support MT-dependent secondary cell wall deposition. Mass spectrometry and immunodetection concurred that detyrosination, non-tyrosination and glutamylation were essentially absent in tubulins isolated from wood-forming tissues of P. deltoides and P. tremula ×alba. Label-free quantification of tubulin isotypes and RNA-Seq estimation of tubulin transcript abundance were largely consistent with transcriptional regulation. However, two TUB isotypes were detected at noticeably lower levels than expected based on RNA-Seq transcript abundance in both Populus species. These findings led us to conclude that MT composition during wood formation depends exclusively on transcriptional and, to a lesser extent, translational regulation of tubulin isotypes.

Research Organization:
Univ. of Georgia, Athens, GA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
SC0008470
OSTI ID:
1423767
Journal Information:
Frontiers in Plant Science, Vol. 7; ISSN 1664-462X
Publisher:
Frontiers Research FoundationCopyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 3 works
Citation information provided by
Web of Science

References (39)

De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis journal July 2013
Drought response transcriptomes are altered in poplar with reduced tonoplast sucrose transporter expression journal September 2016
Post-Translational Modifications to Toxoplasma gondii α- and β-Tubulins Include Novel C-Terminal Methylation journal January 2010
Functional network analysis of genes differentially expressed during xylogenesis in soc1ful woody Arabidopsis plants journal June 2016
The polyglutamylated lateral chain of alpha-tubulin plays a key role in flagellar motility journal June 1996
Post-translational modifications of ?-tubulin in Zea mays L. are highly tissue specific journal January 2004
The novel functions of kinesin motor proteins in plants journal December 2011
Cytoskeletal Motors in Arabidopsis. Sixty-One Kinesins and Seventeen Myosins: Figure 1. journal December 2004
Tubulin perturbation leads to unexpected cell wall modifications and affects stomatal behaviour in Populus journal August 2015
Methods in Tubulin Proteomics book January 2010
Higher plant tubulin identified by self-assembly into microtubules in vitro journal June 1982
Plant cell division is specifically affected by nitrotyrosine journal December 2009
The evolution and diversification of plant microtubule-associated proteins journal May 2013
Acetylation of α-Tubulin on Lys 40 Is a Widespread Post-Translational Modification in Angiosperms journal July 2013
aTRAM - automated target restricted assembly method: a fast method for assembling loci across divergent taxa from next-generation sequencing data journal March 2015
Tension wood as a model for functional genomics of wood formation: Research review journal August 2004
Arabidopsis thaliana histone deacetylase 14 (HDA14) is an α-tubulin deacetylase that associates with PP2A and enriches in the microtubule fraction with the putative histone acetyltransferase ELP3: Arabidopsis PP2A, HDA14 and ELP3 bind microtubules journal May 2012
Post-translational modifications regulate microtubule function journal December 2003
Differential Expansion and Expression of α - and β -Tubulin Gene Families in Populus journal September 2007
MEC-17 is an α-tubulin acetyltransferase journal September 2010
Wood Formation in Trees journal December 2001
Immunological homologues of theArabidopsis thaliana ?1 tubulin are polyglutamylated inNicotiana tabacum journal September 1998
Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels journal January 1996
Cadmium affects microtubule organization and post-translational modifications of tubulin in seedlings of soybean (Glycine max L.) journal November 2015
A label-free mass spectrometry method for relative quantitation of β-tubulin isotype expression in human tumor tissue journal October 2012
Tubulin proteomics: Towards breaking the code journal January 2009
Changes in the accumulation of α- and β-tubulin during bud development in Vitis vinifera L. journal November 2009
Accumulation and post-translational modifications of plant tubulins journal September 2013
Microtubules and the Control of Wood Formation book January 2008
Tubulin Post-Translational Modifications. Enzymes and Their Mechanisms of Action journal March 1997
Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells journal March 1997
Post-translational modifications of tubulin journal May 2014
Fast gapped-read alignment with Bowtie 2 journal March 2012
Characterizing the molecular regulation of inhibitory immune checkpoints with multimodal single-cell screens journal March 2021
Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions journal November 2011
Affinity Purification and Characterization of Functional Tubulin from Cell Suspension Cultures of Arabidopsis and Tobacco journal January 2016
Gibberellic acid stabilises microtubules in maize suspension cells to cold and stimulates acetylation of α-tubulin 1 journal January 1999
Accumulation of DNA damage alters microRNA gene transcription in Arabidopsis thaliana journal December 2022
Microtubule organization in the green kingdom: chaos or self-order? journal April 2002

Cited By (2)

Compensatory Guaiacyl Lignin Biosynthesis at the Expense of Syringyl Lignin in 4CL1 -Knockout Poplar journal March 2020
Evolutionary characterization and transcript profiling of β-tubulin genes in flax (Linum usitatissimum L.) during plant development journal December 2017

Figures / Tables (6)

FIGURE 1(p. 3)figure FIGURE 1
TABLE 1(p. 4)table TABLE 1
FIGURE 2(p. 5)figure FIGURE 2
FIGURE 3(p. 6)figure FIGURE 3
FIGURE 4(p. 7)figure FIGURE 4
FIGURE 5(p. 7)figure FIGURE 5

Similar Records

Related Subjects

54 ENVIRONMENTAL SCIENCES