Microscopic diffusion in hydrated encysted eggs of brine shrimp

Mamontov, Eugene

doi:10.1016/j.bbagen.2017.05.022

Title: Microscopic diffusion in hydrated encysted eggs of brine shrimp

Journal Article · Wed May 24 00:00:00 EDT 2017 · Biochimica et Biophysica Acta - General Subjects

DOI:https://doi.org/10.1016/j.bbagen.2017.05.022· OSTI ID:1408013

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Chemical and Engineering Materials Division

We have studied microscopic diffusion of water in fully hydrated encysted eggs of brine shrimp (Artemia). We utilized quasielastic neutron scattering. Dry eggs of brine shrimp were rehydrated using (1) water without additives, (2) eutectic mixture of water and dimethyl sulfoxide, and (3) a concentrated aqueous solution of lithium chloride. Despite the complexity of the hydrated multicellular organism, measurable microscopic diffusivity of water is rather well defined. Pure hydration water in eggs exhibits freezing temperature depression, whereas hydration water in eggs mixed with dimethyl sulfoxide or lithium chloride does not crystallize at all. The characteristic size of the voids occupied by water or aqueous solvents in hydrated brine shrimp eggs is between 2 and 10 nm. Those voids are accessible to co-solvents such as dimethyl sulfoxide and lithium chloride. There is no evidence of intracellular water in the hydrated eggs. The lack of intracellular water in the fully hydrated (but still under arrested development) state must be linked to the unique resilience against adverse environmental factors documented not only for the anhydrous, but also hydrated encysted eggs of brine shrimp.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1408013

Alternate ID(s):: OSTI ID: 1550096

Journal Information:: Biochimica et Biophysica Acta - General Subjects, Vol. 1861, Issue 9; ISSN 0304-4165

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 6 works

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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
54 ENVIRONMENTAL SCIENCES
Brine shrimp eggs
hydration water
aqueous solvents
microscopic dynamics

Title: Microscopic diffusion in hydrated encysted eggs of brine shrimp

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